Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species
In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra <i>Naja pallida</...
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MDPI AG
2020-08-01
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| Series: | Toxins |
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| author | Ewelina Kuna Aleksandra Bocian Konrad K. Hus Vladimir Petrilla Monika Petrillova Jaroslav Legath Anna Lewinska Maciej Wnuk |
| author_facet | Ewelina Kuna Aleksandra Bocian Konrad K. Hus Vladimir Petrilla Monika Petrillova Jaroslav Legath Anna Lewinska Maciej Wnuk |
| author_sort | Ewelina Kuna |
| collection | DOAJ |
| description | In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra <i>Naja pallida</i> (NPV) and Mozambique spitting cobra <i>Naja mossambica</i> (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three <i>Candida</i> species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of <i>C. albicans</i> and <i>C. tropicalis</i>, affected cell cycle of <i>C. albicans</i>, inhibited <i>C. tropicalis</i>-based biofilm formation and promoted oxidative stress in <i>C. albicans</i>, <i>C. glabrata</i> and <i>C. tropicalis</i> cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three <i>Candida</i> species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of <i>Candida</i> species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate <i>Candida</i> pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments. |
| first_indexed | 2024-03-10T17:58:53Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T17:58:53Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b02e6672002343d6bde4247aaf0f11fc2023-11-20T09:02:52ZengMDPI AGToxins2072-66512020-08-0112850010.3390/toxins12080500Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> SpeciesEwelina Kuna0Aleksandra Bocian1Konrad K. Hus2Vladimir Petrilla3Monika Petrillova4Jaroslav Legath5Anna Lewinska6Maciej Wnuk7Department of Biotechnology, Institute of Biology and Biotechnology, University of Rzeszow, 35-310 Rzeszow, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, PolandDepartment of Physiology, University of Veterinary Medicine and Pharmacy, 041 81 Kosice, Slovak RepublicDepartment of General Education Subjects, University of Veterinary Medicine and Pharmacy, 041 81 Kosice, Slovak RepublicDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, PolandDepartment of Biotechnology, Institute of Biology and Biotechnology, University of Rzeszow, 35-310 Rzeszow, PolandDepartment of Biotechnology, Institute of Biology and Biotechnology, University of Rzeszow, 35-310 Rzeszow, PolandIn contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra <i>Naja pallida</i> (NPV) and Mozambique spitting cobra <i>Naja mossambica</i> (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three <i>Candida</i> species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of <i>C. albicans</i> and <i>C. tropicalis</i>, affected cell cycle of <i>C. albicans</i>, inhibited <i>C. tropicalis</i>-based biofilm formation and promoted oxidative stress in <i>C. albicans</i>, <i>C. glabrata</i> and <i>C. tropicalis</i> cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three <i>Candida</i> species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of <i>Candida</i> species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate <i>Candida</i> pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments.https://www.mdpi.com/2072-6651/12/8/500<i>Candida albicans</i>cobra venomsphospholipase secretionammonia signalingcolony development and aging |
| spellingShingle | Ewelina Kuna Aleksandra Bocian Konrad K. Hus Vladimir Petrilla Monika Petrillova Jaroslav Legath Anna Lewinska Maciej Wnuk Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species Toxins <i>Candida albicans</i> cobra venoms phospholipase secretion ammonia signaling colony development and aging |
| title | Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species |
| title_full | Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species |
| title_fullStr | Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species |
| title_full_unstemmed | Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species |
| title_short | Evaluation of Antifungal Activity of <i>Naja pallida</i> and <i>Naja mossambica</i> Venoms against Three <i>Candida</i> Species |
| title_sort | evaluation of antifungal activity of i naja pallida i and i naja mossambica i venoms against three i candida i species |
| topic | <i>Candida albicans</i> cobra venoms phospholipase secretion ammonia signaling colony development and aging |
| url | https://www.mdpi.com/2072-6651/12/8/500 |
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