Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)

Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical...

Full description

Bibliographic Details
Main Authors: Ikfa Permatasari, Fiddy Semba Prasetiya, Sulastri Arsad, Mochamad Untung Kurnia Agung, Jean-Luc Mouget
Format: Article
Language:English
Published: Jenderal Soedirman University 2019-05-01
Series:Omni-Akuatika
Online Access:http://ojs.omniakuatika.net/index.php/joa/article/view/626
_version_ 1828776936314241024
author Ikfa Permatasari
Fiddy Semba Prasetiya
Sulastri Arsad
Mochamad Untung Kurnia Agung
Jean-Luc Mouget
author_facet Ikfa Permatasari
Fiddy Semba Prasetiya
Sulastri Arsad
Mochamad Untung Kurnia Agung
Jean-Luc Mouget
author_sort Ikfa Permatasari
collection DOAJ
description Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatic
first_indexed 2024-12-11T16:11:20Z
format Article
id doaj.art-b032cbc1c0134b99bcd416fde38d7fb0
institution Directory Open Access Journal
issn 1858-3873
2476-9347
language English
last_indexed 2024-12-11T16:11:20Z
publishDate 2019-05-01
publisher Jenderal Soedirman University
record_format Article
series Omni-Akuatika
spelling doaj.art-b032cbc1c0134b99bcd416fde38d7fb02022-12-22T00:59:04ZengJenderal Soedirman UniversityOmni-Akuatika1858-38732476-93472019-05-01151303810.20884/1.oa.2019.15.1.626178Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)Ikfa Permatasari0Fiddy Semba Prasetiya1Sulastri Arsad2Mochamad Untung Kurnia Agung3Jean-Luc Mouget4Universitas PadjajaranUniversitas PadjajaranUniversitas BrawijayaUniversitas PadjajaranFR CNRS 3473 IUML, Mer-Molécules-Santé (MMS) Le Mans Université, Avenue Olivier Messiaen 72085 Le Mans, FranceHaslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatichttp://ojs.omniakuatika.net/index.php/joa/article/view/626
spellingShingle Ikfa Permatasari
Fiddy Semba Prasetiya
Sulastri Arsad
Mochamad Untung Kurnia Agung
Jean-Luc Mouget
Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)
Omni-Akuatika
title Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)
title_full Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)
title_fullStr Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)
title_full_unstemmed Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)
title_short Antibacterial Activity of <i>Haslea ostrearia</i> Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)
title_sort antibacterial activity of i haslea ostrearia i supernatant adapted in indonesia against pathogenic bacteria relevant to mariculture in vitro study
url http://ojs.omniakuatika.net/index.php/joa/article/view/626
work_keys_str_mv AT ikfapermatasari antibacterialactivityofihasleaostreariaisupernatantadaptedinindonesiaagainstpathogenicbacteriarelevanttomaricultureinvitrostudy
AT fiddysembaprasetiya antibacterialactivityofihasleaostreariaisupernatantadaptedinindonesiaagainstpathogenicbacteriarelevanttomaricultureinvitrostudy
AT sulastriarsad antibacterialactivityofihasleaostreariaisupernatantadaptedinindonesiaagainstpathogenicbacteriarelevanttomaricultureinvitrostudy
AT mochamaduntungkurniaagung antibacterialactivityofihasleaostreariaisupernatantadaptedinindonesiaagainstpathogenicbacteriarelevanttomaricultureinvitrostudy
AT jeanlucmouget antibacterialactivityofihasleaostreariaisupernatantadaptedinindonesiaagainstpathogenicbacteriarelevanttomaricultureinvitrostudy