The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line
Background: Zinc oxide nanoparticles can produce free radicals due to their piezoelectric properties, which are needed for cancer treatment. Also, using ultrasound waves in combination with zinc oxide nanoparticles can help increase the response to sonodynamic therapy by synergistically producing fr...
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| Format: | Article |
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Isfahan University of Medical Sciences
2023-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | https://jims.mui.ac.ir/article_26271_326b5193600f8194d03ff56042864349.pdf |
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| author | Arman Esmailzadeh Ahmad Shanei Neda Attaran Najmeh Najmoddin Atena Shirazi Seyed Hossein Hejazi |
| author_facet | Arman Esmailzadeh Ahmad Shanei Neda Attaran Najmeh Najmoddin Atena Shirazi Seyed Hossein Hejazi |
| author_sort | Arman Esmailzadeh |
| collection | DOAJ |
| description | Background: Zinc oxide nanoparticles can produce free radicals due to their piezoelectric properties, which are needed for cancer treatment. Also, using ultrasound waves in combination with zinc oxide nanoparticles can help increase the response to sonodynamic therapy by synergistically producing free radicals. On the other hand, with gold nanoparticles as a place for cavitation in the ultrasound field, the intensity threshold required for cavitation is reduced, reducing the damage to normal cells. This study aimed to investigate the effect of sonodynamic therapy of melanoma with zinc oxide/gold nanocomposite as a sonosensitizer.
Methods: Chemical dosimetry was used to determine the level of free radicals produced due to combined zinc oxide/gold nanocomposite with and without ultrasound radiation. Also, measuring the survival and apoptosis rate in the cultured melanoma cells was done in-vitro by MTT and flow cytometry tests, respectively.
Findings: With the increase in the production of free radicals, the survival percentage of melanoma cells treated with combined zinc oxide/gold nanocomposite and ultrasound waves at an intensity of 2 W/cm2 showed the most significant decrease (by 20%) which was significantly different from the control group. Also, the apoptosis level caused by sonodynamic therapy with zinc oxide/gold nanocomposite increased significantly.
Conclusion: Using ultrasound waves in combination with zinc oxide/gold nanocomposite can play an influential role in improving the response of melanoma to treatment by raising the level of free radicals and reducing the general toxicity for non-cancerous cells by lowering the cavitation threshold. |
| first_indexed | 2024-03-12T05:15:57Z |
| format | Article |
| id | doaj.art-b03368320c9f4d558d37255c32d3adcc |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T05:15:57Z |
| publishDate | 2023-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-b03368320c9f4d558d37255c32d3adcc2023-09-03T08:09:17ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2023-01-014069591792310.48305/jims.v40.i695.091726271The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell LineArman Esmailzadeh0Ahmad Shanei1Neda Attaran2Najmeh Najmoddin3Atena Shirazi4Seyed Hossein Hejazi5PhD in Medical Physics, Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, IranProfessor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Medical Nanotechnology, Applied Biophotonics Research Center, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranAssistant Professor, Department of Biomedical Engineering, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranMsc in Biomedical Engineering, Department of Biomedical Engineering, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranProfessor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Zinc oxide nanoparticles can produce free radicals due to their piezoelectric properties, which are needed for cancer treatment. Also, using ultrasound waves in combination with zinc oxide nanoparticles can help increase the response to sonodynamic therapy by synergistically producing free radicals. On the other hand, with gold nanoparticles as a place for cavitation in the ultrasound field, the intensity threshold required for cavitation is reduced, reducing the damage to normal cells. This study aimed to investigate the effect of sonodynamic therapy of melanoma with zinc oxide/gold nanocomposite as a sonosensitizer. Methods: Chemical dosimetry was used to determine the level of free radicals produced due to combined zinc oxide/gold nanocomposite with and without ultrasound radiation. Also, measuring the survival and apoptosis rate in the cultured melanoma cells was done in-vitro by MTT and flow cytometry tests, respectively. Findings: With the increase in the production of free radicals, the survival percentage of melanoma cells treated with combined zinc oxide/gold nanocomposite and ultrasound waves at an intensity of 2 W/cm2 showed the most significant decrease (by 20%) which was significantly different from the control group. Also, the apoptosis level caused by sonodynamic therapy with zinc oxide/gold nanocomposite increased significantly. Conclusion: Using ultrasound waves in combination with zinc oxide/gold nanocomposite can play an influential role in improving the response of melanoma to treatment by raising the level of free radicals and reducing the general toxicity for non-cancerous cells by lowering the cavitation threshold.https://jims.mui.ac.ir/article_26271_326b5193600f8194d03ff56042864349.pdfapoptosisgoldnanoparticlesmelanomaultrasonic waveszinc oxide |
| spellingShingle | Arman Esmailzadeh Ahmad Shanei Neda Attaran Najmeh Najmoddin Atena Shirazi Seyed Hossein Hejazi The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line مجله دانشکده پزشکی اصفهان apoptosis gold nanoparticles melanoma ultrasonic waves zinc oxide |
| title | The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line |
| title_full | The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line |
| title_fullStr | The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line |
| title_full_unstemmed | The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line |
| title_short | The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line |
| title_sort | effect of ultrasound therapy in the presence of combined zinc oxide gold nanocomposite on melanoma cell line |
| topic | apoptosis gold nanoparticles melanoma ultrasonic waves zinc oxide |
| url | https://jims.mui.ac.ir/article_26271_326b5193600f8194d03ff56042864349.pdf |
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