UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic
Xueshuantong Lyophilized Powder (XST), consisting of a series of saponins extracted from Panax notoginseng, is widely applied to treat acute cerebral infarction, stroke, and coronary heart disease in China. However, most adverse drug reactions (ADR) in clinic are caused by quality problems of XST. I...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.00633/full |
| _version_ | 1818112418935472128 |
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| author | Zhi-Rui Yang Zi-Hao Wang Zi-Hao Wang Jin-Fa Tang Yan Yan Shi-Jun Yue Wu-Wen Feng Zheng-Yuan Shi Xin-Tong Meng Cheng Peng Chang-Yun Wang Da-Li Meng Dan Yan |
| author_facet | Zhi-Rui Yang Zi-Hao Wang Zi-Hao Wang Jin-Fa Tang Yan Yan Shi-Jun Yue Wu-Wen Feng Zheng-Yuan Shi Xin-Tong Meng Cheng Peng Chang-Yun Wang Da-Li Meng Dan Yan |
| author_sort | Zhi-Rui Yang |
| collection | DOAJ |
| description | Xueshuantong Lyophilized Powder (XST), consisting of a series of saponins extracted from Panax notoginseng, is widely applied to treat acute cerebral infarction, stroke, and coronary heart disease in China. However, most adverse drug reactions (ADR) in clinic are caused by quality problems of XST. In this study, six batches of certainly abnormal, four batches of possibly abnormal XST, and eight batches of normal XST were obtained from the clinical practice. Their quality fluctuations were identified by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF/MSE) and bioassays including antithrombin and proplasmin assay. Fourteen potential components responsible for clinical ADR were identified by UPLC-QTOF/MSE, especially ginsenoside Rg1, Rg3, Rb1 and notoginsenoside R1. In addition, 83.3% (5/6) and 50.0% (3/6) certainly abnormal samples could be identified by UPLC-QTOF/MSE and bioassay, respectively. Interestingly, further integration of the two methods could entirely identify all the certainly abnormal samples and inferred that all the possibly abnormal samples were closely related to their quality fluctuation. It indicates that it is advisable to combine UPLC-QTOF/MSE and bioassay for identifying quality fluctuation of XST, and thus reduce its ADR in clinic. |
| first_indexed | 2024-12-11T03:18:38Z |
| format | Article |
| id | doaj.art-b03412c3647f4ea695aff420c6653fe6 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T03:18:38Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-b03412c3647f4ea695aff420c6653fe62022-12-22T01:22:41ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-06-01910.3389/fphar.2018.00633376909UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in ClinicZhi-Rui Yang0Zi-Hao Wang1Zi-Hao Wang2Jin-Fa Tang3Yan Yan4Shi-Jun Yue5Wu-Wen Feng6Zheng-Yuan Shi7Xin-Tong Meng8Cheng Peng9Chang-Yun Wang10Da-Li Meng11Dan Yan12Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, ChinaDepartment of Pharmacy, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, ChinaBeijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaBeijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaKey Laboratory of Marine Drugs (Ministry of Education of China), School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, ChinaBeijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaXueshuantong Lyophilized Powder (XST), consisting of a series of saponins extracted from Panax notoginseng, is widely applied to treat acute cerebral infarction, stroke, and coronary heart disease in China. However, most adverse drug reactions (ADR) in clinic are caused by quality problems of XST. In this study, six batches of certainly abnormal, four batches of possibly abnormal XST, and eight batches of normal XST were obtained from the clinical practice. Their quality fluctuations were identified by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF/MSE) and bioassays including antithrombin and proplasmin assay. Fourteen potential components responsible for clinical ADR were identified by UPLC-QTOF/MSE, especially ginsenoside Rg1, Rg3, Rb1 and notoginsenoside R1. In addition, 83.3% (5/6) and 50.0% (3/6) certainly abnormal samples could be identified by UPLC-QTOF/MSE and bioassay, respectively. Interestingly, further integration of the two methods could entirely identify all the certainly abnormal samples and inferred that all the possibly abnormal samples were closely related to their quality fluctuation. It indicates that it is advisable to combine UPLC-QTOF/MSE and bioassay for identifying quality fluctuation of XST, and thus reduce its ADR in clinic.https://www.frontiersin.org/article/10.3389/fphar.2018.00633/fullXueshuantong lyophilized powderquality fluctuationadverse drug reactionUPLC-QTOF/MSEbioassay |
| spellingShingle | Zhi-Rui Yang Zi-Hao Wang Zi-Hao Wang Jin-Fa Tang Yan Yan Shi-Jun Yue Wu-Wen Feng Zheng-Yuan Shi Xin-Tong Meng Cheng Peng Chang-Yun Wang Da-Li Meng Dan Yan UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic Frontiers in Pharmacology Xueshuantong lyophilized powder quality fluctuation adverse drug reaction UPLC-QTOF/MSE bioassay |
| title | UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic |
| title_full | UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic |
| title_fullStr | UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic |
| title_full_unstemmed | UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic |
| title_short | UPLC-QTOF/MSE and Bioassay Are Available Approaches for Identifying Quality Fluctuation of Xueshuantong Lyophilized Powder in Clinic |
| title_sort | uplc qtof mse and bioassay are available approaches for identifying quality fluctuation of xueshuantong lyophilized powder in clinic |
| topic | Xueshuantong lyophilized powder quality fluctuation adverse drug reaction UPLC-QTOF/MSE bioassay |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.00633/full |
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