Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology
Legionella pneumophila is a rarely diagnosed microorganism in Serbia. It causes legionellosis, usually a mild respiratory infection. However, in some cases it can be severe and even life threatening. In June 2020, during the COVID-19 pandemic, a patient with symptoms of the aforesaid infection, name...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-05-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X221095035 |
| _version_ | 1811253506698379264 |
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| author | Milica Jovanović Nikola Mitrović Letitia Beraud Nikola Trboljevac Branko Milošević Aleksandra Radovanović Spurnić Snežana Jovanović Dragana Marić |
| author_facet | Milica Jovanović Nikola Mitrović Letitia Beraud Nikola Trboljevac Branko Milošević Aleksandra Radovanović Spurnić Snežana Jovanović Dragana Marić |
| author_sort | Milica Jovanović |
| collection | DOAJ |
| description | Legionella pneumophila is a rarely diagnosed microorganism in Serbia. It causes legionellosis, usually a mild respiratory infection. However, in some cases it can be severe and even life threatening. In June 2020, during the COVID-19 pandemic, a patient with symptoms of the aforesaid infection, namely severe pneumonia and acute respiratory distress syndrome, was admitted to the hospital. The multiplex polymerase chain reaction (PCR) test (The BioFire FilmArray Pneumonia Panel plus) detected the presence of L. pneumophila in the patient’s bronchial secretions. The specific culture for the detection of that organism, however, remained sterile. The patient’s paired sera had been sent for serology and the results in both of them came back positive for Legionella spp . 1–6, while the assays specific for each one of the 10 serogroups detected more than a fourfold increase of antibody titers in an uncommon serogroup 2 only. The patient was treated with moxifloxacin; he recovered well and was discharged after 26 days of hospitalization. Having being diagnosed with the L. pneumophila infection correctly through the multiplex PCR test, the patient was given the right therapy with moxifloxacin. The serologic assays corroborated this result and revealed the uncommon group 2, thus confirming the necessity of carrying out all the tests available to attain the exact diagnosis of legionellosis. |
| first_indexed | 2024-04-12T16:52:00Z |
| format | Article |
| id | doaj.art-b0341319c46e476ca2a852b4a8037d34 |
| institution | Directory Open Access Journal |
| issn | 2058-7392 |
| language | English |
| last_indexed | 2024-04-12T16:52:00Z |
| publishDate | 2022-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-b0341319c46e476ca2a852b4a8037d342022-12-22T03:24:22ZengSAGE PublishingEuropean Journal of Inflammation2058-73922022-05-012010.1177/1721727X221095035Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serologyMilica Jovanović0Nikola Mitrović1Letitia Beraud2Nikola Trboljevac3Branko Milošević4Aleksandra Radovanović Spurnić5Snežana Jovanović6Dragana Marić7Department of Medical Microbiology, Clinical Center of Serbia, University Clinical Center of Serbia, Belgrade, SerbiaClinic for Infectious and Tropical Diseases, Belgrade, SerbiaLaboratoire de Biologie Medicale Multi Sites du Chu de Lyon, Lyon, FrancePulmonology Clinic, Clinical Center of Serbia, Belgrade, SerbiaClinic for Infectious and Tropical Diseases, Belgrade, SerbiaClinic for Infectious and Tropical Diseases, Belgrade, SerbiaDepartment of Medical Microbiology, Clinical Center of Serbia, University Clinical Center of Serbia, Belgrade, SerbiaPulmonology Clinic, Clinical Center of Serbia, Belgrade, SerbiaLegionella pneumophila is a rarely diagnosed microorganism in Serbia. It causes legionellosis, usually a mild respiratory infection. However, in some cases it can be severe and even life threatening. In June 2020, during the COVID-19 pandemic, a patient with symptoms of the aforesaid infection, namely severe pneumonia and acute respiratory distress syndrome, was admitted to the hospital. The multiplex polymerase chain reaction (PCR) test (The BioFire FilmArray Pneumonia Panel plus) detected the presence of L. pneumophila in the patient’s bronchial secretions. The specific culture for the detection of that organism, however, remained sterile. The patient’s paired sera had been sent for serology and the results in both of them came back positive for Legionella spp . 1–6, while the assays specific for each one of the 10 serogroups detected more than a fourfold increase of antibody titers in an uncommon serogroup 2 only. The patient was treated with moxifloxacin; he recovered well and was discharged after 26 days of hospitalization. Having being diagnosed with the L. pneumophila infection correctly through the multiplex PCR test, the patient was given the right therapy with moxifloxacin. The serologic assays corroborated this result and revealed the uncommon group 2, thus confirming the necessity of carrying out all the tests available to attain the exact diagnosis of legionellosis.https://doi.org/10.1177/1721727X221095035 |
| spellingShingle | Milica Jovanović Nikola Mitrović Letitia Beraud Nikola Trboljevac Branko Milošević Aleksandra Radovanović Spurnić Snežana Jovanović Dragana Marić Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology European Journal of Inflammation |
| title | Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology |
| title_full | Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology |
| title_fullStr | Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology |
| title_full_unstemmed | Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology |
| title_short | Severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology |
| title_sort | severe pneumonia caused by detected by a multiplex polymerase chain reaction assay and confirmed by serology |
| url | https://doi.org/10.1177/1721727X221095035 |
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