Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes
Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed us...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2024-02-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/70/2/70_2023-067/_pdf/-char/en |
| _version_ | 1797224488790654976 |
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| author | Naomi NAKAGATA Satohiro NAKAO Nobuyuki MIKODA Katsuma YAMAGA Toru TAKEO |
| author_facet | Naomi NAKAGATA Satohiro NAKAO Nobuyuki MIKODA Katsuma YAMAGA Toru TAKEO |
| author_sort | Naomi NAKAGATA |
| collection | DOAJ |
| description | Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen–thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1–13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection. |
| first_indexed | 2024-04-24T13:53:55Z |
| format | Article |
| id | doaj.art-b035477e0d91437eb1856a4064ad4a07 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-04-24T13:53:55Z |
| publishDate | 2024-02-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-b035477e0d91437eb1856a4064ad4a072024-04-03T23:50:29ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002024-02-0170212313010.1262/jrd.2023-067jrdTime elapsed between ovulation and insemination determines the quality of fertilized rat oocytesNaomi NAKAGATA0Satohiro NAKAO1Nobuyuki MIKODA2Katsuma YAMAGA3Toru TAKEO4Division of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanDivision of Reproductive Engineering, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanDivision of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanDivision of Reproductive Engineering, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanDivision of Reproductive Engineering, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanGenetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen–thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1–13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection.https://www.jstage.jst.go.jp/article/jrd/70/2/70_2023-067/_pdf/-char/enembryo transferin vitro fertilizationovulationpostovulatory oocyte agingrat |
| spellingShingle | Naomi NAKAGATA Satohiro NAKAO Nobuyuki MIKODA Katsuma YAMAGA Toru TAKEO Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes The Journal of Reproduction and Development embryo transfer in vitro fertilization ovulation postovulatory oocyte aging rat |
| title | Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes |
| title_full | Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes |
| title_fullStr | Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes |
| title_full_unstemmed | Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes |
| title_short | Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes |
| title_sort | time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes |
| topic | embryo transfer in vitro fertilization ovulation postovulatory oocyte aging rat |
| url | https://www.jstage.jst.go.jp/article/jrd/70/2/70_2023-067/_pdf/-char/en |
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