Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation
Abstract The purpose of this study was to investigate the expression and clinical value of microRNA-451a (miR-451a) in septic patients and analyze its effect on sepsis-associated cardiac dysfunction and inflammation response. A rat model of sepsis was constructed by cecal ligation and puncture. The...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2020-11-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600106&tlng=en |
| _version_ | 1798015538027298816 |
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| author | Heng Wang Wenjuan Cui Lujun Qiao Guoxin Hu |
| author_facet | Heng Wang Wenjuan Cui Lujun Qiao Guoxin Hu |
| author_sort | Heng Wang |
| collection | DOAJ |
| description | Abstract The purpose of this study was to investigate the expression and clinical value of microRNA-451a (miR-451a) in septic patients and analyze its effect on sepsis-associated cardiac dysfunction and inflammation response. A rat model of sepsis was constructed by cecal ligation and puncture. The expression of miR-451a was measured by quantitative real-time PCR. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of serum miR-451a. The cardiac function and inflammatory responses in septic rats were measured to explore the functional role of miR-451a. Serum expression of miR-451a was increased in septic patients compared with healthy controls, and had the ability to distinguish septic patients from healthy volunteers with a sensitivity and specificity of 87.8% and 81.5%, respectively. Elevated serum miR-451a was associated with sepsis severity, as evidenced by the increased expression of miR-451a in septic shock patients and its correlation with key clinical indicators. Significantly upregulated expression of miR-451a was found in septic patients with cardiac dysfunction, and the knockdown of miR-451a in sepsis rats improved cardiac function and inhibited inflammatory responses. All the data revealed that serum miR-451a serves as a candidate diagnostic biomarker of sepsis and a potential parameter to indicate disease severity. The reduction of miR-451a may mitigate sepsis-induced cardiac dysfunction and inflammatory responses. |
| first_indexed | 2024-04-11T15:36:01Z |
| format | Article |
| id | doaj.art-b036b268fbc5487ab6e76b7d613edbf9 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-04-11T15:36:01Z |
| publishDate | 2020-11-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-b036b268fbc5487ab6e76b7d613edbf92022-12-22T04:15:58ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852020-11-0143410.1590/1678-4685-gmb-2020-0009Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammationHeng WangWenjuan CuiLujun QiaoGuoxin Huhttps://orcid.org/0000-0003-3870-2422Abstract The purpose of this study was to investigate the expression and clinical value of microRNA-451a (miR-451a) in septic patients and analyze its effect on sepsis-associated cardiac dysfunction and inflammation response. A rat model of sepsis was constructed by cecal ligation and puncture. The expression of miR-451a was measured by quantitative real-time PCR. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of serum miR-451a. The cardiac function and inflammatory responses in septic rats were measured to explore the functional role of miR-451a. Serum expression of miR-451a was increased in septic patients compared with healthy controls, and had the ability to distinguish septic patients from healthy volunteers with a sensitivity and specificity of 87.8% and 81.5%, respectively. Elevated serum miR-451a was associated with sepsis severity, as evidenced by the increased expression of miR-451a in septic shock patients and its correlation with key clinical indicators. Significantly upregulated expression of miR-451a was found in septic patients with cardiac dysfunction, and the knockdown of miR-451a in sepsis rats improved cardiac function and inhibited inflammatory responses. All the data revealed that serum miR-451a serves as a candidate diagnostic biomarker of sepsis and a potential parameter to indicate disease severity. The reduction of miR-451a may mitigate sepsis-induced cardiac dysfunction and inflammatory responses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600106&tlng=enMicroRNA-451sepsisdiagnosisinflammationcardiac dysfunction |
| spellingShingle | Heng Wang Wenjuan Cui Lujun Qiao Guoxin Hu Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation Genetics and Molecular Biology MicroRNA-451 sepsis diagnosis inflammation cardiac dysfunction |
| title | Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation |
| title_full | Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation |
| title_fullStr | Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation |
| title_full_unstemmed | Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation |
| title_short | Overexpression of miR-451a in sepsis and septic shock patients is involved in the regulation of sepsis-associated cardiac dysfunction and inflammation |
| title_sort | overexpression of mir 451a in sepsis and septic shock patients is involved in the regulation of sepsis associated cardiac dysfunction and inflammation |
| topic | MicroRNA-451 sepsis diagnosis inflammation cardiac dysfunction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600106&tlng=en |
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