Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay
The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were e...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2014-06-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300016&lng=en&tlng=en |
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| author | Marcelo Fabiano Gomes Boriollo Marielly Reis Resende Thaísla Andrielle da Silva Juliana Yoshida Públio Luiz Silva Souza Carlos Tadeu dos Santos Dias Nelma de Mello Silva Oliveira João Evangelista Fiorini |
| author_facet | Marcelo Fabiano Gomes Boriollo Marielly Reis Resende Thaísla Andrielle da Silva Juliana Yoshida Públio Luiz Silva Souza Carlos Tadeu dos Santos Dias Nelma de Mello Silva Oliveira João Evangelista Fiorini |
| author_sort | Marcelo Fabiano Gomes Boriollo |
| collection | DOAJ |
| description | The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24-48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg-1 and DXR - 5 mg.kg-1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5-2 g.kg-1), GEZJ (2 g.kg-1) + NEU and GEZJ (2 g.kg-1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis of the polychromatic:normochromatic erythrocyte ratio revealed significant differences in the responses to doses of 0.5 g.kg-1 and 1-2 g.kg-1 and the positive control (NEU). These results indicated no systemic toxicity and moderate toxicity at lower and higher doses of GEZJ. The lack of mutagenicity and systemic toxicity in the antimutagenic assays, especially for treatment with GEZJ + DXR, suggested that phytochemical compounds in Z. joazeiro bark attenuated DXR-induced mutagenicity and the moderate systemic toxicity of a high dose of Z. joazeiro bark (2 g.kg-1). Further studies on the genotoxicity of Z. joazeiro extracts are necessary to establish the possible health risk in humans and to determine the potential as a chemopreventive agent for therapeutic use. |
| first_indexed | 2024-12-11T05:51:45Z |
| format | Article |
| id | doaj.art-b03be55b653b4da9b254b21d6e61bf54 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T05:51:45Z |
| publishDate | 2014-06-01 |
| publisher | Sociedade Brasileira de Genética |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-b03be55b653b4da9b254b21d6e61bf542022-12-22T01:18:48ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852014-06-0137242843810.1590/S1415-47572014000300016S1415-47572014000300016Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assayMarcelo Fabiano Gomes Boriollo0Marielly Reis Resende1Thaísla Andrielle da Silva2Juliana Yoshida Públio3Luiz Silva Souza4Carlos Tadeu dos Santos Dias5Nelma de Mello Silva Oliveira6João Evangelista Fiorini7Universidade José do Rosário VellanoUniversidade de São PauloUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade de São PauloUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoThe aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24-48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg-1 and DXR - 5 mg.kg-1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5-2 g.kg-1), GEZJ (2 g.kg-1) + NEU and GEZJ (2 g.kg-1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis of the polychromatic:normochromatic erythrocyte ratio revealed significant differences in the responses to doses of 0.5 g.kg-1 and 1-2 g.kg-1 and the positive control (NEU). These results indicated no systemic toxicity and moderate toxicity at lower and higher doses of GEZJ. The lack of mutagenicity and systemic toxicity in the antimutagenic assays, especially for treatment with GEZJ + DXR, suggested that phytochemical compounds in Z. joazeiro bark attenuated DXR-induced mutagenicity and the moderate systemic toxicity of a high dose of Z. joazeiro bark (2 g.kg-1). Further studies on the genotoxicity of Z. joazeiro extracts are necessary to establish the possible health risk in humans and to determine the potential as a chemopreventive agent for therapeutic use.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300016&lng=en&tlng=enantimutagenicitybone marrowdoxorubicinmicronucleus assaymutagenicityZizyphus joazeiro Mart. (raspa-de-Juá) |
| spellingShingle | Marcelo Fabiano Gomes Boriollo Marielly Reis Resende Thaísla Andrielle da Silva Juliana Yoshida Públio Luiz Silva Souza Carlos Tadeu dos Santos Dias Nelma de Mello Silva Oliveira João Evangelista Fiorini Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay Genetics and Molecular Biology antimutagenicity bone marrow doxorubicin micronucleus assay mutagenicity Zizyphus joazeiro Mart. (raspa-de-Juá) |
| title | Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay |
| title_full | Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay |
| title_fullStr | Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay |
| title_full_unstemmed | Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay |
| title_short | Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay |
| title_sort | evaluation of the mutagenicity and antimutagenicity of ziziphus joazeiro mart bark in the micronucleus assay |
| topic | antimutagenicity bone marrow doxorubicin micronucleus assay mutagenicity Zizyphus joazeiro Mart. (raspa-de-Juá) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300016&lng=en&tlng=en |
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