Ultrasensitive immunochromatographic strip for detection of cyproheptadine
Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1490395 |
| _version_ | 1818931399184351232 |
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| author | Mengyuan Guo Li Sun Liqiang Liu Shanshan Song Hua Kuang Gang Cui |
| author_facet | Mengyuan Guo Li Sun Liqiang Liu Shanshan Song Hua Kuang Gang Cui |
| author_sort | Mengyuan Guo |
| collection | DOAJ |
| description | Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine were 80–120%. An ultrasensitive immunochromatographic strip, based on the antibody, was developed for the rapid detection of cyproheptadine in pig urine. The test strip had a cut-off value of 5 ng/mL. |
| first_indexed | 2024-12-20T04:15:58Z |
| format | Article |
| id | doaj.art-b03f7302b7ad4d049525ce3e778582bf |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-20T04:15:58Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-b03f7302b7ad4d049525ce3e778582bf2022-12-21T19:53:46ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129194195210.1080/09540105.2018.14903951490395Ultrasensitive immunochromatographic strip for detection of cyproheptadineMengyuan Guo0Li Sun1Liqiang Liu2Shanshan Song3Hua Kuang4Gang Cui5State Key Laboratory of Food Science and Technology, Jiangnan UniversityChinese Academy of Inspection and QuarantineState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversitySchool of Marine and Biological Engineering, Yancheng Teachers UniversityUsing immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine were 80–120%. An ultrasensitive immunochromatographic strip, based on the antibody, was developed for the rapid detection of cyproheptadine in pig urine. The test strip had a cut-off value of 5 ng/mL.http://dx.doi.org/10.1080/09540105.2018.1490395cyproheptadineindirect competitive enzyme-linked immunosorbent assay (ic-elisa)immunochromatographic test stripurine |
| spellingShingle | Mengyuan Guo Li Sun Liqiang Liu Shanshan Song Hua Kuang Gang Cui Ultrasensitive immunochromatographic strip for detection of cyproheptadine Food and Agricultural Immunology cyproheptadine indirect competitive enzyme-linked immunosorbent assay (ic-elisa) immunochromatographic test strip urine |
| title | Ultrasensitive immunochromatographic strip for detection of cyproheptadine |
| title_full | Ultrasensitive immunochromatographic strip for detection of cyproheptadine |
| title_fullStr | Ultrasensitive immunochromatographic strip for detection of cyproheptadine |
| title_full_unstemmed | Ultrasensitive immunochromatographic strip for detection of cyproheptadine |
| title_short | Ultrasensitive immunochromatographic strip for detection of cyproheptadine |
| title_sort | ultrasensitive immunochromatographic strip for detection of cyproheptadine |
| topic | cyproheptadine indirect competitive enzyme-linked immunosorbent assay (ic-elisa) immunochromatographic test strip urine |
| url | http://dx.doi.org/10.1080/09540105.2018.1490395 |
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