A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity
Wenrong Zhang,1,2,* Haining Wang,1,* Tong Wu,1 Xintao Gao,1 Yuting Shang,1 Zhifang Zhang,1 Xingjian Liu,1 Yinü Li1 1National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China; 2...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2024-03-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/a-sars-cov-2-nanobody-displayed-on-the-surface-of-human-ferritin-with--peer-reviewed-fulltext-article-IJN |
| _version_ | 1797266514264457216 |
|---|---|
| author | Zhang W Wang H Wu T Gao X Shang Y Zhang Z Liu X Li Y |
| author_facet | Zhang W Wang H Wu T Gao X Shang Y Zhang Z Liu X Li Y |
| author_sort | Zhang W |
| collection | DOAJ |
| description | Wenrong Zhang,1,2,* Haining Wang,1,* Tong Wu,1 Xintao Gao,1 Yuting Shang,1 Zhifang Zhang,1 Xingjian Liu,1 Yinü Li1 1National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China; 2College of Life Sciences, Capital Normal University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xingjian Liu; Yinü Li, Tel/Fax +861082109854 ; +861082105495, Email liuxingjian@caas.cn; liyinv@caas.cnPurpose: COVID-19 is rampant throughout the world, which has caused great damage to human lives and seriously hindered the development of the global economy. Aiming at the treatment of SARS-CoV-2, in this study, we proposed a novel fenobody strategy based on ferritin (Fe) self-assembly technology.Methods: The neutralizing nanobody H11-D4 of SARS-CoV-2 fused to the C-terminus of end-modified human ferritin was expressed in E. coli and silkworm baculovirus expression systems. A large number of nanoparticles were successfully self-assembled in silkworms, while relatively few nanoparticles can be observed in the treated products from E. coli by electron microscopy. Subsequently, the fenobody’s expression level and neutralizing activity were then evaluated.Results: The results showed that the IC50 of H11-D4 and fenobody Fe-H11-D4 expressed in E. coli were 171.1 nmol L− 1 and 20.87 nmol L− 1, respectively. However, the IC50 of Fe-HD11-D4 expressed in silkworms was 1.46 nmol L− 1 showing better neutralization activity.Conclusion: Therefore, fenobodies can be well self-assembled in silkworm baculovirus expression system, and ferritin self-assembly technology can effectively improve nanobody neutralization activity.Keywords: COVID-19, ferritin, fenobody, baculovirus expression system, silkworm |
| first_indexed | 2024-04-25T01:01:54Z |
| format | Article |
| id | doaj.art-b0409644df85422fb3fd46d0d27502cd |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-04-25T01:01:54Z |
| publishDate | 2024-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-b0409644df85422fb3fd46d0d27502cd2024-03-10T16:38:18ZengDove Medical PressInternational Journal of Nanomedicine1178-20132024-03-01Volume 192429244091079A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization ActivityZhang WWang HWu TGao XShang YZhang ZLiu XLi YWenrong Zhang,1,2,* Haining Wang,1,* Tong Wu,1 Xintao Gao,1 Yuting Shang,1 Zhifang Zhang,1 Xingjian Liu,1 Yinü Li1 1National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China; 2College of Life Sciences, Capital Normal University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xingjian Liu; Yinü Li, Tel/Fax +861082109854 ; +861082105495, Email liuxingjian@caas.cn; liyinv@caas.cnPurpose: COVID-19 is rampant throughout the world, which has caused great damage to human lives and seriously hindered the development of the global economy. Aiming at the treatment of SARS-CoV-2, in this study, we proposed a novel fenobody strategy based on ferritin (Fe) self-assembly technology.Methods: The neutralizing nanobody H11-D4 of SARS-CoV-2 fused to the C-terminus of end-modified human ferritin was expressed in E. coli and silkworm baculovirus expression systems. A large number of nanoparticles were successfully self-assembled in silkworms, while relatively few nanoparticles can be observed in the treated products from E. coli by electron microscopy. Subsequently, the fenobody’s expression level and neutralizing activity were then evaluated.Results: The results showed that the IC50 of H11-D4 and fenobody Fe-H11-D4 expressed in E. coli were 171.1 nmol L− 1 and 20.87 nmol L− 1, respectively. However, the IC50 of Fe-HD11-D4 expressed in silkworms was 1.46 nmol L− 1 showing better neutralization activity.Conclusion: Therefore, fenobodies can be well self-assembled in silkworm baculovirus expression system, and ferritin self-assembly technology can effectively improve nanobody neutralization activity.Keywords: COVID-19, ferritin, fenobody, baculovirus expression system, silkwormhttps://www.dovepress.com/a-sars-cov-2-nanobody-displayed-on-the-surface-of-human-ferritin-with--peer-reviewed-fulltext-article-IJNcovid-19ferritinfenobodybaculovirus expression systemsilkworm |
| spellingShingle | Zhang W Wang H Wu T Gao X Shang Y Zhang Z Liu X Li Y A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity International Journal of Nanomedicine covid-19 ferritin fenobody baculovirus expression system silkworm |
| title | A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity |
| title_full | A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity |
| title_fullStr | A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity |
| title_full_unstemmed | A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity |
| title_short | A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity |
| title_sort | sars cov 2 nanobody displayed on the surface of human ferritin with high neutralization activity |
| topic | covid-19 ferritin fenobody baculovirus expression system silkworm |
| url | https://www.dovepress.com/a-sars-cov-2-nanobody-displayed-on-the-surface-of-human-ferritin-with--peer-reviewed-fulltext-article-IJN |
| work_keys_str_mv | AT zhangw asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT wangh asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT wut asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT gaox asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT shangy asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT zhangz asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT liux asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT liy asarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT zhangw sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT wangh sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT wut sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT gaox sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT shangy sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT zhangz sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT liux sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity AT liy sarscov2nanobodydisplayedonthesurfaceofhumanferritinwithhighneutralizationactivity |