Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice
One of the most promising techniques for oxidation of glucose into a gluconic acid is the utilization of the enzyme glucose oxidase. In order to optimize the process, two types of enzymes were used as catalysts for glucose oxidation in several model synthetic grape juices. The first one is a food gr...
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| Format: | Article |
| Language: | English |
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University of Banja Luka - Faculty of Agriculture, Banja Luka
2016-01-01
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| Series: | Agroznanje |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/2233-0070/2016/2233-00701601047P.pdf |
| _version_ | 1797205095731953664 |
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| author | Petkova Verica Mladenoska Irina Gjuladin-Hellon Teuta |
| author_facet | Petkova Verica Mladenoska Irina Gjuladin-Hellon Teuta |
| author_sort | Petkova Verica |
| collection | DOAJ |
| description | One of the most promising techniques for oxidation of glucose into a gluconic acid is the utilization of the enzyme glucose oxidase. In order to optimize the process, two types of enzymes were used as catalysts for glucose oxidation in several model synthetic grape juices. The first one is a food grade enzyme Alphamalt Gloxy 5080 from Aspergillus Niger. The other one is pure enzyme from Aspergillus Niger, used as a sole or in a combination with catalase isolated from beef liver. Both the pure glucose solution and the synthetic grape juice were used as substrates for enzymatic pretreatment. The Alphamalt Gloxy 5080 enzyme, used in a concentration of 1 g/L, showed 77.60% substrate conversion of the glucose used in a concentration of 10 g/L. the pure glucose oxidase having concentration of 25 mg/L converted only 1.32% of glucose, while when combined with 15 mL catalase, the conversion was even 49.25%. |
| first_indexed | 2024-04-24T08:45:41Z |
| format | Article |
| id | doaj.art-b0424afabb5b49298223830e1d9e1696 |
| institution | Directory Open Access Journal |
| issn | 2233-0070 |
| language | English |
| last_indexed | 2024-04-24T08:45:41Z |
| publishDate | 2016-01-01 |
| publisher | University of Banja Luka - Faculty of Agriculture, Banja Luka |
| record_format | Article |
| series | Agroznanje |
| spelling | doaj.art-b0424afabb5b49298223830e1d9e16962024-04-16T13:36:40ZengUniversity of Banja Luka - Faculty of Agriculture, Banja LukaAgroznanje2233-00702016-01-01171475810.7251/AGREN1601047P2233-00701601047PUtilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juicePetkova Verica0Mladenoska Irina1Gjuladin-Hellon Teuta2University of Ss. Cyril and Methodius, Faculty of Technology and Metallurgy, Skopje, Republic of MacedoniaUniversity of Ss. Cyril and Methodius, Faculty of Technology and Metallurgy, Skopje, Republic of MacedoniaSs. Cyril and Methodius University in Skopje, Faculty of Agricultural Sciences and Food, Skopje, MacedoniaOne of the most promising techniques for oxidation of glucose into a gluconic acid is the utilization of the enzyme glucose oxidase. In order to optimize the process, two types of enzymes were used as catalysts for glucose oxidation in several model synthetic grape juices. The first one is a food grade enzyme Alphamalt Gloxy 5080 from Aspergillus Niger. The other one is pure enzyme from Aspergillus Niger, used as a sole or in a combination with catalase isolated from beef liver. Both the pure glucose solution and the synthetic grape juice were used as substrates for enzymatic pretreatment. The Alphamalt Gloxy 5080 enzyme, used in a concentration of 1 g/L, showed 77.60% substrate conversion of the glucose used in a concentration of 10 g/L. the pure glucose oxidase having concentration of 25 mg/L converted only 1.32% of glucose, while when combined with 15 mL catalase, the conversion was even 49.25%.https://scindeks-clanci.ceon.rs/data/pdf/2233-0070/2016/2233-00701601047P.pdfglucoseglucose oxidaseoxidation of glucosegrape juiceenzymatic treatment |
| spellingShingle | Petkova Verica Mladenoska Irina Gjuladin-Hellon Teuta Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice Agroznanje glucose glucose oxidase oxidation of glucose grape juice enzymatic treatment |
| title | Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice |
| title_full | Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice |
| title_fullStr | Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice |
| title_full_unstemmed | Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice |
| title_short | Utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice |
| title_sort | utilization of different types of glucose oxidase for reduction of glucose concentration in synthetic grape juice |
| topic | glucose glucose oxidase oxidation of glucose grape juice enzymatic treatment |
| url | https://scindeks-clanci.ceon.rs/data/pdf/2233-0070/2016/2233-00701601047P.pdf |
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