3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering
Abstract Cellulose nanocrystals (CNC) are drawing increasing attention in the fields of biomedicine and healthcare owing to their durability, biocompatibility, biodegradability and excellent mechanical properties. Herein, we fabricated using fused deposition modelling technology 3D composite scaffol...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25652-x |
| _version_ | 1828170224089694208 |
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| author | Kanga Marius N’Gatta Habib Belaid Joelle El Hayek Edja Florentin Assanvo Marilyn Kajdan Nathalie Masquelez David Boa Vincent Cavaillès Mikhael Bechelany Chrystelle Salameh |
| author_facet | Kanga Marius N’Gatta Habib Belaid Joelle El Hayek Edja Florentin Assanvo Marilyn Kajdan Nathalie Masquelez David Boa Vincent Cavaillès Mikhael Bechelany Chrystelle Salameh |
| author_sort | Kanga Marius N’Gatta |
| collection | DOAJ |
| description | Abstract Cellulose nanocrystals (CNC) are drawing increasing attention in the fields of biomedicine and healthcare owing to their durability, biocompatibility, biodegradability and excellent mechanical properties. Herein, we fabricated using fused deposition modelling technology 3D composite scaffolds from polylactic acid (PLA) and CNC extracted from Ficus thonningii. Scanning electron microscopy revealed that the printed scaffolds exhibit interconnected pores with an estimated average pore size of approximately 400 µm. Incorporating 3% (w/w) of CNC into the composite improved PLA mechanical properties (Young's modulus increased by ~ 30%) and wettability (water contact angle decreased by ~ 17%). The mineralization process of printed scaffolds using simulated body fluid was validated and nucleation of hydroxyapatite confirmed. Additionally, cytocompatibility tests revealed that PLA and CNC-based PLA scaffolds are non-toxic and compatible with bone cells. Our design, based on rapid 3D printing of PLA/CNC composites, combines the ability to control the architecture and provide improved mechanical and biological properties of the scaffolds, which opens perspectives for applications in bone tissue engineering and in regenerative medicine. |
| first_indexed | 2024-04-12T03:05:13Z |
| format | Article |
| id | doaj.art-b04644981c4848c490031b5a7029030b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T03:05:13Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b04644981c4848c490031b5a7029030b2022-12-22T03:50:32ZengNature PortfolioScientific Reports2045-23222022-12-0112111410.1038/s41598-022-25652-x3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineeringKanga Marius N’Gatta0Habib Belaid1Joelle El Hayek2Edja Florentin Assanvo3Marilyn Kajdan4Nathalie Masquelez5David Boa6Vincent Cavaillès7Mikhael Bechelany8Chrystelle Salameh9Institut Européen des Membranes, IEM, UMR 5635, Univ Montpellier, ENSCM, CNRSInstitut Européen des Membranes, IEM, UMR 5635, Univ Montpellier, ENSCM, CNRSInstitut Européen des Membranes, IEM, UMR 5635, Univ Montpellier, ENSCM, CNRSLaboratoire de Thermodynamique et de Physico-Chimie du Milieu, UFR SFA, Université Nangui AbrogouaIRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université MontpellierInstitut Européen des Membranes, IEM, UMR 5635, Univ Montpellier, ENSCM, CNRSLaboratoire de Thermodynamique et de Physico-Chimie du Milieu, UFR SFA, Université Nangui AbrogouaIRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université MontpellierInstitut Européen des Membranes, IEM, UMR 5635, Univ Montpellier, ENSCM, CNRSInstitut Européen des Membranes, IEM, UMR 5635, Univ Montpellier, ENSCM, CNRSAbstract Cellulose nanocrystals (CNC) are drawing increasing attention in the fields of biomedicine and healthcare owing to their durability, biocompatibility, biodegradability and excellent mechanical properties. Herein, we fabricated using fused deposition modelling technology 3D composite scaffolds from polylactic acid (PLA) and CNC extracted from Ficus thonningii. Scanning electron microscopy revealed that the printed scaffolds exhibit interconnected pores with an estimated average pore size of approximately 400 µm. Incorporating 3% (w/w) of CNC into the composite improved PLA mechanical properties (Young's modulus increased by ~ 30%) and wettability (water contact angle decreased by ~ 17%). The mineralization process of printed scaffolds using simulated body fluid was validated and nucleation of hydroxyapatite confirmed. Additionally, cytocompatibility tests revealed that PLA and CNC-based PLA scaffolds are non-toxic and compatible with bone cells. Our design, based on rapid 3D printing of PLA/CNC composites, combines the ability to control the architecture and provide improved mechanical and biological properties of the scaffolds, which opens perspectives for applications in bone tissue engineering and in regenerative medicine.https://doi.org/10.1038/s41598-022-25652-x |
| spellingShingle | Kanga Marius N’Gatta Habib Belaid Joelle El Hayek Edja Florentin Assanvo Marilyn Kajdan Nathalie Masquelez David Boa Vincent Cavaillès Mikhael Bechelany Chrystelle Salameh 3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering Scientific Reports |
| title | 3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering |
| title_full | 3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering |
| title_fullStr | 3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering |
| title_full_unstemmed | 3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering |
| title_short | 3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering |
| title_sort | 3d printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering |
| url | https://doi.org/10.1038/s41598-022-25652-x |
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