Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis?
Due to the lack of effective medical treatment for non-alcoholic steatohepatitis (NASH), we aimed to evaluate new treatment options. In particular, our goal was to investigate and compare the effects of N-acetylcysteine (NAC) and Adalimumab treatment on tumor necrosis factor alpha (TNF-α) and oxidat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-08-01
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| Series: | Gastroenterology Insights |
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| Online Access: | http://www.pagepress.org/journals/index.php/gi/article/view/6227 |
| _version_ | 1811181505346535424 |
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| author | Mustafa Yalçın Muhammed Ali Kıyak Mesut Akarsu Aslı Çelik Göksel Bengi Özgül Sağol |
| author_facet | Mustafa Yalçın Muhammed Ali Kıyak Mesut Akarsu Aslı Çelik Göksel Bengi Özgül Sağol |
| author_sort | Mustafa Yalçın |
| collection | DOAJ |
| description | Due to the lack of effective medical treatment for non-alcoholic steatohepatitis (NASH), we aimed to evaluate new treatment options. In particular, our goal was to investigate and compare the effects of N-acetylcysteine (NAC) and Adalimumab treatment on tumor necrosis factor alpha (TNF-α) and oxidative stress during the development of NASH in a rat model of the disease. Our study included a total of 35 female Wistar albino rats that were divided into 5 groups of 7 each, and evaluated over a 6 week period. One group received a normal diet, while the other four groups received a methionine and choline deficient (MCD) diet. One of the groups receiving the MCD diet did not take any medicine, while the other three were administered NAC, adalimumab, or a NAC/adalimumab combination therapy. NASH was successfully established in the MCD diet group. Levels of TNF-α were effectively suppressed in the three groups that received therapy. Even though adalimumab significantly enhanced suppression of TNF-α, the NASH score was suppressed to a more statistically significant extent in the groups receiving NAC. Our study showed that TNF-α and oxidative stress play an important role in NASH pathogenesis. The antioxidant agent, NAC, was found to be superior to the anti-TNF agent, Adalimumab, in the improvement of total NASH score. Although these drugs did not prevent the development of NASH, it was shown that they mildly reverse the NASH histopathology score, suggesting improvement of and overall liver function. |
| first_indexed | 2024-04-11T09:18:24Z |
| format | Article |
| id | doaj.art-b046bac419b94424be29d49bcec2552f |
| institution | Directory Open Access Journal |
| issn | 2036-7414 2036-7422 |
| language | English |
| last_indexed | 2024-04-11T09:18:24Z |
| publishDate | 2016-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gastroenterology Insights |
| spelling | doaj.art-b046bac419b94424be29d49bcec2552f2022-12-22T04:32:17ZengMDPI AGGastroenterology Insights2036-74142036-74222016-08-017110.4081/gi.2016.62273495Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis?Mustafa Yalçın0Muhammed Ali Kıyak1Mesut Akarsu2Aslı Çelik3Göksel Bengi4Özgül Sağol5Department of Gastroenterology, Dokuz Eylul University Faculty of Medicine, IzmirDepartment of Gastroenterology, Dokuz Eylul University Faculty of Medicine, IzmirDepartment of Gastroenterology, Dokuz Eylul University Faculty of Medicine, IzmirDepartment of Animal Laboratory, Dokuz Eylul University, IzmirDepartment of Gastroenterology, Dokuz Eylul University Faculty of Medicine, IzmirDepartment of Pathology, Dokuz Eylul University, IzmirDue to the lack of effective medical treatment for non-alcoholic steatohepatitis (NASH), we aimed to evaluate new treatment options. In particular, our goal was to investigate and compare the effects of N-acetylcysteine (NAC) and Adalimumab treatment on tumor necrosis factor alpha (TNF-α) and oxidative stress during the development of NASH in a rat model of the disease. Our study included a total of 35 female Wistar albino rats that were divided into 5 groups of 7 each, and evaluated over a 6 week period. One group received a normal diet, while the other four groups received a methionine and choline deficient (MCD) diet. One of the groups receiving the MCD diet did not take any medicine, while the other three were administered NAC, adalimumab, or a NAC/adalimumab combination therapy. NASH was successfully established in the MCD diet group. Levels of TNF-α were effectively suppressed in the three groups that received therapy. Even though adalimumab significantly enhanced suppression of TNF-α, the NASH score was suppressed to a more statistically significant extent in the groups receiving NAC. Our study showed that TNF-α and oxidative stress play an important role in NASH pathogenesis. The antioxidant agent, NAC, was found to be superior to the anti-TNF agent, Adalimumab, in the improvement of total NASH score. Although these drugs did not prevent the development of NASH, it was shown that they mildly reverse the NASH histopathology score, suggesting improvement of and overall liver function.http://www.pagepress.org/journals/index.php/gi/article/view/6227Non-alcoholic steatohepatitis, N-acetylcysteine, Adalimumab, tumor necrosis factor alpha. |
| spellingShingle | Mustafa Yalçın Muhammed Ali Kıyak Mesut Akarsu Aslı Çelik Göksel Bengi Özgül Sağol Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis? Gastroenterology Insights Non-alcoholic steatohepatitis, N-acetylcysteine, Adalimumab, tumor necrosis factor alpha. |
| title | Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis? |
| title_full | Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis? |
| title_fullStr | Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis? |
| title_full_unstemmed | Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis? |
| title_short | Are N-acetylcysteine and adalimumab effective for non-alcoholic steatohepatitis? |
| title_sort | are n acetylcysteine and adalimumab effective for non alcoholic steatohepatitis |
| topic | Non-alcoholic steatohepatitis, N-acetylcysteine, Adalimumab, tumor necrosis factor alpha. |
| url | http://www.pagepress.org/journals/index.php/gi/article/view/6227 |
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