Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection
ABSTRACTHemerocallis citrina Baroni (daylily) is an important cash crop in many regions of China. Daylily rust caused by Puccinia hemerocallidis (Ph) is a major disease in H. citrina. Rust infection on the leaves of two resistant and susceptible varieties of H. citrina was examined. Transcriptome se...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2023.2260410 |
| _version_ | 1797245142086713344 |
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| author | Lijie Zhang Lingling Zhou Jiali Meng Shaojun Wu Shuhua Liu Nianfu Yang Fufa Tian Xiang Yu |
| author_facet | Lijie Zhang Lingling Zhou Jiali Meng Shaojun Wu Shuhua Liu Nianfu Yang Fufa Tian Xiang Yu |
| author_sort | Lijie Zhang |
| collection | DOAJ |
| description | ABSTRACTHemerocallis citrina Baroni (daylily) is an important cash crop in many regions of China. Daylily rust caused by Puccinia hemerocallidis (Ph) is a major disease in H. citrina. Rust infection on the leaves of two resistant and susceptible varieties of H. citrina was examined. Transcriptome sequencing and defense enzyme activity were used to analyze the differential expression of related genes after 0, 72, 144, 192, and 288 h, to reveal the response mechanism of H. citrina to rust infection and screen-related resistance genes. The results suggested that 72 h after Ph infection was the key time point for determining differences between resistant and susceptible varieties. DEGs were significantly enriched in phenylpropanoid biosynthesis, plant–pathogen interaction, plant hormone signal transduction, and the MAPK signaling pathway. TFs WRKY33 and CYP98A are key genes closely related to the disease-resistance response of H. citrina. This study is the first large-scale transcriptomic analysis of H. citrina. |
| first_indexed | 2024-03-11T10:27:54Z |
| format | Article |
| id | doaj.art-b04d901d9b8447b4a418467dec378ad3 |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-04-24T19:22:12Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-b04d901d9b8447b4a418467dec378ad32024-03-25T17:45:03ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532023-12-0118110.1080/17429145.2023.2260410Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infectionLijie Zhang0Lingling Zhou1Jiali Meng2Shaojun Wu3Shuhua Liu4Nianfu Yang5Fufa Tian6Xiang Yu7Institute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaInstitute of Suqian, Jiangsu Academy of Agricultural Sciences, Suqian, People’s Republic of ChinaABSTRACTHemerocallis citrina Baroni (daylily) is an important cash crop in many regions of China. Daylily rust caused by Puccinia hemerocallidis (Ph) is a major disease in H. citrina. Rust infection on the leaves of two resistant and susceptible varieties of H. citrina was examined. Transcriptome sequencing and defense enzyme activity were used to analyze the differential expression of related genes after 0, 72, 144, 192, and 288 h, to reveal the response mechanism of H. citrina to rust infection and screen-related resistance genes. The results suggested that 72 h after Ph infection was the key time point for determining differences between resistant and susceptible varieties. DEGs were significantly enriched in phenylpropanoid biosynthesis, plant–pathogen interaction, plant hormone signal transduction, and the MAPK signaling pathway. TFs WRKY33 and CYP98A are key genes closely related to the disease-resistance response of H. citrina. This study is the first large-scale transcriptomic analysis of H. citrina.https://www.tandfonline.com/doi/10.1080/17429145.2023.2260410Daylilyenzymatic activitiesRNA-seqtranscription factorsresistance genes |
| spellingShingle | Lijie Zhang Lingling Zhou Jiali Meng Shaojun Wu Shuhua Liu Nianfu Yang Fufa Tian Xiang Yu Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection Journal of Plant Interactions Daylily enzymatic activities RNA-seq transcription factors resistance genes |
| title | Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection |
| title_full | Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection |
| title_fullStr | Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection |
| title_full_unstemmed | Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection |
| title_short | Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection |
| title_sort | comparative transcriptome analysis of the resistance mechanism of hemerocallis citrina baroni to puccinia hemerocallidis infection |
| topic | Daylily enzymatic activities RNA-seq transcription factors resistance genes |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2023.2260410 |
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