Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway
Cerebral ischemia reperfusion injury (CIRI) is a great challenge for the patients with brain ischemia, but its pathophysiological mechanism has not been clearly explored. This study aims to decipher the effect of chrysin and plasminogen activator urokinase (PLAU) in CIRI. The immune-related genes we...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320421000845 |
| _version_ | 1828288804734107648 |
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| author | Ning Li Ying Liu Ju-Rong Li Wen-Xue Zhang |
| author_facet | Ning Li Ying Liu Ju-Rong Li Wen-Xue Zhang |
| author_sort | Ning Li |
| collection | DOAJ |
| description | Cerebral ischemia reperfusion injury (CIRI) is a great challenge for the patients with brain ischemia, but its pathophysiological mechanism has not been clearly explored. This study aims to decipher the effect of chrysin and plasminogen activator urokinase (PLAU) in CIRI. The immune-related genes were collected from the ImmPort website, and the differentially expressed genes were determined based on the Gene Expression Omnibus (GEO) database. PC12 cells were used to establish an ischemic stroke model under the condition of oxygen-glucose deprivation and reoxygenation (OGD/R). Small interfering RNA strategy was employed to knock down the PLAU expression of PC12 cells. The proliferation and apoptosis rates of PC12 cells treated by OGD/R or/and chrysin were detected with Cell Counting Kit 8 (CCK-8) and flow cytometry. The protein and mRNA expressions were measured using western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR). PLAU was identified as a candidate for CIRI treatment and expressed at higher levels in CIRI tissues compared with that in normal controls. Chrysin was determined as a crucial agent that could decrease the expression of PLAU. Chrysin significantly promoted the cell proliferation, inhibited the protein levels of PLAU, p–NF–κB, and p-IKκB in PC12 cells after OGD/R. Silencing of PLAU strengthened the protective effect of chrysin on PC12 cells treated by OGD/R, including the improvement of cell viability and suppression of apoptosis. Chrysin inactivated the NF-κB pathway via targeting PLAU in OGD/R-stimulated PC12 cells. Chrysin prevented PC12 cells from OGD/R-stimulated damage via decreasing PLAU expression and inactivating the NF-κB signaling pathway. |
| first_indexed | 2024-04-13T10:11:01Z |
| format | Article |
| id | doaj.art-b04e33a410f840e186bb267cc0420676 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-04-13T10:11:01Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-b04e33a410f840e186bb267cc04206762022-12-22T02:50:56ZengElsevierRegenerative Therapy2352-32042022-03-01196976Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathwayNing Li0Ying Liu1Ju-Rong Li2Wen-Xue Zhang3Department of Rehabilitation, Traditional Chinese Medicine Hospital, Zibo, Shandong Province, 255300, ChinaDepartment of Neurology, Gaotang County People's Hospital, Liaocheng, Shandong Province, 252800, ChinaDepartment of Geriatrics, Dazhou Central Hospital, Dazhou, Sichuan Province, 635000, ChinaDepartment of Neurosurgery, Weifang People's Hospital, Weifang, Shandong Province, 261000, China; Corresponding author. Department of Neurosurgery, Weifang People's Hospital, No.151 Guangwen Street, Kuiwen District, Weifang, Shandong Province, 261000, China.Cerebral ischemia reperfusion injury (CIRI) is a great challenge for the patients with brain ischemia, but its pathophysiological mechanism has not been clearly explored. This study aims to decipher the effect of chrysin and plasminogen activator urokinase (PLAU) in CIRI. The immune-related genes were collected from the ImmPort website, and the differentially expressed genes were determined based on the Gene Expression Omnibus (GEO) database. PC12 cells were used to establish an ischemic stroke model under the condition of oxygen-glucose deprivation and reoxygenation (OGD/R). Small interfering RNA strategy was employed to knock down the PLAU expression of PC12 cells. The proliferation and apoptosis rates of PC12 cells treated by OGD/R or/and chrysin were detected with Cell Counting Kit 8 (CCK-8) and flow cytometry. The protein and mRNA expressions were measured using western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR). PLAU was identified as a candidate for CIRI treatment and expressed at higher levels in CIRI tissues compared with that in normal controls. Chrysin was determined as a crucial agent that could decrease the expression of PLAU. Chrysin significantly promoted the cell proliferation, inhibited the protein levels of PLAU, p–NF–κB, and p-IKκB in PC12 cells after OGD/R. Silencing of PLAU strengthened the protective effect of chrysin on PC12 cells treated by OGD/R, including the improvement of cell viability and suppression of apoptosis. Chrysin inactivated the NF-κB pathway via targeting PLAU in OGD/R-stimulated PC12 cells. Chrysin prevented PC12 cells from OGD/R-stimulated damage via decreasing PLAU expression and inactivating the NF-κB signaling pathway.http://www.sciencedirect.com/science/article/pii/S2352320421000845ChrysinOxygen-glucose deprivation and reoxygenationPlasminogen activator urokinaseCell viabilityApoptosis |
| spellingShingle | Ning Li Ying Liu Ju-Rong Li Wen-Xue Zhang Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway Regenerative Therapy Chrysin Oxygen-glucose deprivation and reoxygenation Plasminogen activator urokinase Cell viability Apoptosis |
| title | Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway |
| title_full | Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway |
| title_fullStr | Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway |
| title_full_unstemmed | Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway |
| title_short | Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway |
| title_sort | chrysin which targets plau protects pc12 cells from ogd r stimulated damage through repressing the nf κb signaling pathway |
| topic | Chrysin Oxygen-glucose deprivation and reoxygenation Plasminogen activator urokinase Cell viability Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2352320421000845 |
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