Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i>
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a <i>Trf</i> (<i>PxTrf</i>) from <i>Plutella xylostella</i> was identified and characterized. The <i>PxTrf</i> consisted of a 2046-bp open read...
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MDPI AG
2020-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/5/281 |
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| author | Huihui Xu Zhongping Hao Lifang Wang Shuangjiao Li Yuruo Guo Xiangli Dang |
| author_facet | Huihui Xu Zhongping Hao Lifang Wang Shuangjiao Li Yuruo Guo Xiangli Dang |
| author_sort | Huihui Xu |
| collection | DOAJ |
| description | Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a <i>Trf</i> (<i>PxTrf</i>) from <i>Plutella xylostella</i> was identified and characterized. The <i>PxTrf</i> consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although <i>PxTrf</i> was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. <i>PxTrf</i> transcript level was highest in fat bodies among various tissues. The <i>PxTrf</i> transcript levels increased significantly after the stimulation of pathogens. A decrease in <i>PxTrf</i> expression via RNA interference enhanced the susceptibility of <i>P. xylostella</i> to the <i>Isaria cicadae</i> fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in <i>P. xylostella</i> immunity against fungal infection and insect development. |
| first_indexed | 2024-03-10T20:02:38Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T20:02:38Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj.art-b052c4aa3cab4a7f8a4e7267bdc2a32d2023-11-19T23:30:01ZengMDPI AGInsects2075-44502020-05-0111528110.3390/insects11050281Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i>Huihui Xu0Zhongping Hao1Lifang Wang2Shuangjiao Li3Yuruo Guo4Xiangli Dang5Key Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaCrop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaKey Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaKey Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaKey Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaTransferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a <i>Trf</i> (<i>PxTrf</i>) from <i>Plutella xylostella</i> was identified and characterized. The <i>PxTrf</i> consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although <i>PxTrf</i> was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. <i>PxTrf</i> transcript level was highest in fat bodies among various tissues. The <i>PxTrf</i> transcript levels increased significantly after the stimulation of pathogens. A decrease in <i>PxTrf</i> expression via RNA interference enhanced the susceptibility of <i>P. xylostella</i> to the <i>Isaria cicadae</i> fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in <i>P. xylostella</i> immunity against fungal infection and insect development.https://www.mdpi.com/2075-4450/11/5/281<i>Plutella xylostella</i><i>Isaria cicadae</i>transferrinexpression patternsimmunitydevelopment |
| spellingShingle | Huihui Xu Zhongping Hao Lifang Wang Shuangjiao Li Yuruo Guo Xiangli Dang Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i> Insects <i>Plutella xylostella</i> <i>Isaria cicadae</i> transferrin expression patterns immunity development |
| title | Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i> |
| title_full | Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i> |
| title_fullStr | Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i> |
| title_full_unstemmed | Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i> |
| title_short | Suppression of <i>Transferrin</i> Expression Enhances the Susceptibility of <i>Plutella xylostella</i> to <i>Isaria cicadae</i> |
| title_sort | suppression of i transferrin i expression enhances the susceptibility of i plutella xylostella i to i isaria cicadae i |
| topic | <i>Plutella xylostella</i> <i>Isaria cicadae</i> transferrin expression patterns immunity development |
| url | https://www.mdpi.com/2075-4450/11/5/281 |
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