A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro
Abstract Background Rhipicephalus microplus is a hard tick species that has a high impact on cattle health and production in tropical and subtropical regions. Recently, ribosomal DNA and morphological analysis resulted in the reinstatement of R. australis as a separate species from R. microplus. Bot...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3616-3 |
| _version_ | 1818383541377957888 |
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| author | Jos J. A. Trentelman Hendry Teunissen Jos A. G. M. Kleuskens Jos van de Crommert José de la Fuente Joppe W. R. Hovius Theo P. M. Schetters |
| author_facet | Jos J. A. Trentelman Hendry Teunissen Jos A. G. M. Kleuskens Jos van de Crommert José de la Fuente Joppe W. R. Hovius Theo P. M. Schetters |
| author_sort | Jos J. A. Trentelman |
| collection | DOAJ |
| description | Abstract Background Rhipicephalus microplus is a hard tick species that has a high impact on cattle health and production in tropical and subtropical regions. Recently, ribosomal DNA and morphological analysis resulted in the reinstatement of R. australis as a separate species from R. microplus. Both feed on cattle and can transmit bovine pathogens such as Anaplasma and Babesia species. The current treatment with acaricides is becoming increasingly less effective due to the emergence of resistant tick strains. A promising alternative can be found in the form of anti-tick vaccines. The available commercial vaccines can be used to control tick infestation, but the lack of a knockdown effect (> 90% reduction in tick numbers as seen with effective acaricides) hampers its widespread use, hence higher efficacious vaccines are needed. Instead of searching for new protective antigens, we investigated the efficacy of vaccines that contain more than one (partially) protective antigen. For screening vaccine formulations, a previously developed in vitro feeding assay was used in which R. australis larvae are fed sera that were raised against the candidate vaccine antigens. In the present study, the efficacy of the Bm86 midgut antigen and the cytosolic Subolesin (SUB) antigen were evaluated in vitro. Results Antiserum against recombinant Bm86 (rBm86) partially inhibited larval engorgement, whereas antiserum against recombinant SUB (rSUB) did not have any effect on feeding of larvae. Importantly, when larvae were fed a combination of antiserum against rBm86 and rSUB, a synergistic effect on significantly reducing larval infestations was found. Immunohistochemical analysis revealed that the rBm86 antiserum reacted with gut epithelium of R. australis larvae, whereas the antiserum against rSUB stained salivary glands and rectal sac epithelium. Conclusions Combining anti-Bm86 and anti-subolesin antibodies synergistically reduced R. australis larval feeding in vitro. Rhipicephalus australis is a one host tick, meaning that the larvae develop to nymphs and subsequently adults on the same host. Hence, this protective effect could be even more pronounced when larvae are used for infestation of vaccinated cattle, as the antibodies could then affect all three developmental stages. This will be tested in future in vivo experiments. |
| first_indexed | 2024-12-14T03:08:00Z |
| format | Article |
| id | doaj.art-b055151c92954c8c95ce3eb7fc744233 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-14T03:08:00Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-b055151c92954c8c95ce3eb7fc7442332022-12-21T23:19:21ZengBMCParasites & Vectors1756-33052019-07-0112111010.1186/s13071-019-3616-3A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitroJos J. A. Trentelman0Hendry Teunissen1Jos A. G. M. Kleuskens2Jos van de Crommert3José de la Fuente4Joppe W. R. Hovius5Theo P. M. Schetters6Center for Experimental and Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam UMC, Univ of AmsterdamMérieux NutriSciencesMSD Animal HealthAduro Biotech EuropeSaBio. Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM)Center for Experimental and Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam UMC, Univ of AmsterdamProtActivityAbstract Background Rhipicephalus microplus is a hard tick species that has a high impact on cattle health and production in tropical and subtropical regions. Recently, ribosomal DNA and morphological analysis resulted in the reinstatement of R. australis as a separate species from R. microplus. Both feed on cattle and can transmit bovine pathogens such as Anaplasma and Babesia species. The current treatment with acaricides is becoming increasingly less effective due to the emergence of resistant tick strains. A promising alternative can be found in the form of anti-tick vaccines. The available commercial vaccines can be used to control tick infestation, but the lack of a knockdown effect (> 90% reduction in tick numbers as seen with effective acaricides) hampers its widespread use, hence higher efficacious vaccines are needed. Instead of searching for new protective antigens, we investigated the efficacy of vaccines that contain more than one (partially) protective antigen. For screening vaccine formulations, a previously developed in vitro feeding assay was used in which R. australis larvae are fed sera that were raised against the candidate vaccine antigens. In the present study, the efficacy of the Bm86 midgut antigen and the cytosolic Subolesin (SUB) antigen were evaluated in vitro. Results Antiserum against recombinant Bm86 (rBm86) partially inhibited larval engorgement, whereas antiserum against recombinant SUB (rSUB) did not have any effect on feeding of larvae. Importantly, when larvae were fed a combination of antiserum against rBm86 and rSUB, a synergistic effect on significantly reducing larval infestations was found. Immunohistochemical analysis revealed that the rBm86 antiserum reacted with gut epithelium of R. australis larvae, whereas the antiserum against rSUB stained salivary glands and rectal sac epithelium. Conclusions Combining anti-Bm86 and anti-subolesin antibodies synergistically reduced R. australis larval feeding in vitro. Rhipicephalus australis is a one host tick, meaning that the larvae develop to nymphs and subsequently adults on the same host. Hence, this protective effect could be even more pronounced when larvae are used for infestation of vaccinated cattle, as the antibodies could then affect all three developmental stages. This will be tested in future in vivo experiments.http://link.springer.com/article/10.1186/s13071-019-3616-3Artificial tick feedingIn vitro screeningR. microplusR. australisSubolesinBm86 |
| spellingShingle | Jos J. A. Trentelman Hendry Teunissen Jos A. G. M. Kleuskens Jos van de Crommert José de la Fuente Joppe W. R. Hovius Theo P. M. Schetters A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro Parasites & Vectors Artificial tick feeding In vitro screening R. microplus R. australis Subolesin Bm86 |
| title | A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro |
| title_full | A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro |
| title_fullStr | A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro |
| title_full_unstemmed | A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro |
| title_short | A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro |
| title_sort | combination of antibodies against bm86 and subolesin inhibits engorgement of rhipicephalus australis formerly rhipicephalus microplus larvae in vitro |
| topic | Artificial tick feeding In vitro screening R. microplus R. australis Subolesin Bm86 |
| url | http://link.springer.com/article/10.1186/s13071-019-3616-3 |
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