A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets
The objective of this study was to assess the effect of a novel corn-expressed phytase (CEP) on growth, nutrients digestibility, bone characteristics and fecal microbiota of pigs fed with very low-protein, -calcium (Ca) and -phosphorous (P) diets. Forty-eight barrows were subjected to 6 groups for 4...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/10/1926 |
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| author | Cedrick N. Shili Jonathan N. Broomhead Shelby C. Spring Mike B. Lanahan Adel Pezeshki |
| author_facet | Cedrick N. Shili Jonathan N. Broomhead Shelby C. Spring Mike B. Lanahan Adel Pezeshki |
| author_sort | Cedrick N. Shili |
| collection | DOAJ |
| description | The objective of this study was to assess the effect of a novel corn-expressed phytase (CEP) on growth, nutrients digestibility, bone characteristics and fecal microbiota of pigs fed with very low-protein, -calcium (Ca) and -phosphorous (P) diets. Forty-eight barrows were subjected to 6 groups for 4 weeks: positive control-adequate protein (PC), negative control-reduced protein (NC), NC + low-dose CEP, i.e., 2000 FTU/kg (LD), NC + high-dose CEP, i.e., 4000 FTU/kg (HD), LD with 0.12% unit reduced Ca and 0.15% unit reduced available P (LDR), and HD with 0.12% unit reduced Ca and 0.15% unit reduced available P (HDR). Compared to NC, LD and HDR had a higher average daily gain (ADG) and gain:protein ratio (G:P), HD and HDR had greater apparent fecal digestibility of Ca and P and bone mineral density and LDR and HDR had lower serum osteocalcin. The feces of LD was enriched in Lachnospiraceae, while the HD had a higher abundance of <i>Succinvibrio</i> and LDR had a higher abundance of <i>Bifidobacterium</i> and Actinobacteria. In conclusion, supplementation of protein-restricted diets with a CEP decreased their negative effects on ADG and G:P ratio, increased the digestibility of Ca and P regardless of the levels of these minerals in the diet, improved bone characteristics and produced differential effects on fecal bacterial population. |
| first_indexed | 2024-03-10T15:28:53Z |
| format | Article |
| id | doaj.art-b057ceb946fc4ec98af90a404babfe72 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T15:28:53Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b057ceb946fc4ec98af90a404babfe722023-11-20T17:48:16ZengMDPI AGAnimals2076-26152020-10-011010192610.3390/ani10101926A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein DietsCedrick N. Shili0Jonathan N. Broomhead1Shelby C. Spring2Mike B. Lanahan3Adel Pezeshki4Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USAAgrivida, Woburn, MA 01801, USADepartment of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USAAgrivida, Woburn, MA 01801, USADepartment of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USAThe objective of this study was to assess the effect of a novel corn-expressed phytase (CEP) on growth, nutrients digestibility, bone characteristics and fecal microbiota of pigs fed with very low-protein, -calcium (Ca) and -phosphorous (P) diets. Forty-eight barrows were subjected to 6 groups for 4 weeks: positive control-adequate protein (PC), negative control-reduced protein (NC), NC + low-dose CEP, i.e., 2000 FTU/kg (LD), NC + high-dose CEP, i.e., 4000 FTU/kg (HD), LD with 0.12% unit reduced Ca and 0.15% unit reduced available P (LDR), and HD with 0.12% unit reduced Ca and 0.15% unit reduced available P (HDR). Compared to NC, LD and HDR had a higher average daily gain (ADG) and gain:protein ratio (G:P), HD and HDR had greater apparent fecal digestibility of Ca and P and bone mineral density and LDR and HDR had lower serum osteocalcin. The feces of LD was enriched in Lachnospiraceae, while the HD had a higher abundance of <i>Succinvibrio</i> and LDR had a higher abundance of <i>Bifidobacterium</i> and Actinobacteria. In conclusion, supplementation of protein-restricted diets with a CEP decreased their negative effects on ADG and G:P ratio, increased the digestibility of Ca and P regardless of the levels of these minerals in the diet, improved bone characteristics and produced differential effects on fecal bacterial population.https://www.mdpi.com/2076-2615/10/10/1926low-protein dietslow calcium/phosphorous dietscorn-expressed phytasegrowthnutrients digestibilitybone characteristics |
| spellingShingle | Cedrick N. Shili Jonathan N. Broomhead Shelby C. Spring Mike B. Lanahan Adel Pezeshki A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets Animals low-protein diets low calcium/phosphorous diets corn-expressed phytase growth nutrients digestibility bone characteristics |
| title | A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets |
| title_full | A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets |
| title_fullStr | A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets |
| title_full_unstemmed | A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets |
| title_short | A Novel Corn-Expressed Phytase Improves Daily Weight Gain, Protein Efficiency Ratio and Nutrients Digestibility and Alters Fecal Microbiota in Pigs Fed with Very Low Protein Diets |
| title_sort | novel corn expressed phytase improves daily weight gain protein efficiency ratio and nutrients digestibility and alters fecal microbiota in pigs fed with very low protein diets |
| topic | low-protein diets low calcium/phosphorous diets corn-expressed phytase growth nutrients digestibility bone characteristics |
| url | https://www.mdpi.com/2076-2615/10/10/1926 |
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