Proteomic analysis of fertilized egg white during early incubation
Proteomic analysis of egg white proteins was performed to elucidate their metabolic fates during first days of embryo development using 2-DE coupled with a LC–MS/MS. A total of 91 protein spots were analyzed, representing 37 proteins belonging to ‘Gallus gallus’, of 19 proteins were detected in egg...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-03-01
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| Series: | EuPA Open Proteomics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212968513000081 |
| _version_ | 1818534817634975744 |
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| author | Jiapei Wang Jianping Wu |
| author_facet | Jiapei Wang Jianping Wu |
| author_sort | Jiapei Wang |
| collection | DOAJ |
| description | Proteomic analysis of egg white proteins was performed to elucidate their metabolic fates during first days of embryo development using 2-DE coupled with a LC–MS/MS. A total of 91 protein spots were analyzed, representing 37 proteins belonging to ‘Gallus gallus’, of 19 proteins were detected in egg whites for the first time, such as lipoproteins, vitellogenin and zona pellucida C protein. All ovomucoid spots with one exception were significantly (P < 0.05) increased. Marker protein and one flavoprotein spot were significantly increased while hemopexin, serum albumin precursor, Ex-FABP precursor and Galline Ex-FABP were significantly decreased. |
| first_indexed | 2024-12-11T18:16:32Z |
| format | Article |
| id | doaj.art-b05e4bf08cd44b70aa65635236cc122e |
| institution | Directory Open Access Journal |
| issn | 2212-9685 |
| language | English |
| last_indexed | 2024-12-11T18:16:32Z |
| publishDate | 2014-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EuPA Open Proteomics |
| spelling | doaj.art-b05e4bf08cd44b70aa65635236cc122e2022-12-22T00:55:25ZengElsevierEuPA Open Proteomics2212-96852014-03-012C385910.1016/j.euprot.2013.11.001Proteomic analysis of fertilized egg white during early incubationJiapei WangJianping WuProteomic analysis of egg white proteins was performed to elucidate their metabolic fates during first days of embryo development using 2-DE coupled with a LC–MS/MS. A total of 91 protein spots were analyzed, representing 37 proteins belonging to ‘Gallus gallus’, of 19 proteins were detected in egg whites for the first time, such as lipoproteins, vitellogenin and zona pellucida C protein. All ovomucoid spots with one exception were significantly (P < 0.05) increased. Marker protein and one flavoprotein spot were significantly increased while hemopexin, serum albumin precursor, Ex-FABP precursor and Galline Ex-FABP were significantly decreased.http://www.sciencedirect.com/science/article/pii/S2212968513000081Fertilized egg whites2-DEProteomicsIncubationQuantificationEmbryo |
| spellingShingle | Jiapei Wang Jianping Wu Proteomic analysis of fertilized egg white during early incubation EuPA Open Proteomics Fertilized egg whites 2-DE Proteomics Incubation Quantification Embryo |
| title | Proteomic analysis of fertilized egg white during early incubation |
| title_full | Proteomic analysis of fertilized egg white during early incubation |
| title_fullStr | Proteomic analysis of fertilized egg white during early incubation |
| title_full_unstemmed | Proteomic analysis of fertilized egg white during early incubation |
| title_short | Proteomic analysis of fertilized egg white during early incubation |
| title_sort | proteomic analysis of fertilized egg white during early incubation |
| topic | Fertilized egg whites 2-DE Proteomics Incubation Quantification Embryo |
| url | http://www.sciencedirect.com/science/article/pii/S2212968513000081 |
| work_keys_str_mv | AT jiapeiwang proteomicanalysisoffertilizedeggwhiteduringearlyincubation AT jianpingwu proteomicanalysisoffertilizedeggwhiteduringearlyincubation |