Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae)
Abstract Background The cattle farming parasite Rhipicephalus australis is the main tick and one of the most important in the world from an economic point of view. Various studies have been developed in order to find plant extracts with effective acaricidal properties and environmentally friendly. S...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-03-01
|
| Series: | Chemical and Biological Technologies in Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40538-023-00389-5 |
| _version_ | 1797865349108989952 |
|---|---|
| author | Marine Toussirot Paul Coulerie Thomas Hüe Alexandre Maciuk Valérie Kagy |
| author_facet | Marine Toussirot Paul Coulerie Thomas Hüe Alexandre Maciuk Valérie Kagy |
| author_sort | Marine Toussirot |
| collection | DOAJ |
| description | Abstract Background The cattle farming parasite Rhipicephalus australis is the main tick and one of the most important in the world from an economic point of view. Various studies have been developed in order to find plant extracts with effective acaricidal properties and environmentally friendly. Studies involving plant extracts for parasite control on commercial animal herds is a developing area in New Caledonia. Bioactive natural products play an important role as lead compounds in the development of new pesticides. Results The ethanolic extract of Piper nigrum L. dried fruits as well as the ethyl acetate extract and the methanolic extract of stems exhibited 100% larvicidal activity (50 mg/mL) against Rh. australis larvae, the cattle tick, an hematophagous parasite. Bioguided fractionation of the ethanolic extract of dried mature fruits using the same assay led to the isolation of five compounds belonging to piperamide family. The structures of isolated compounds were elucidated using spectroscopic methods: ESI-HRMS, 1H- and 13C-NMR spectral data, including DEPT and 2D-NMR experiments (COSY, HSQC, HMBC, and NOESY). These include 1 compound described for the first time in P. nigrum, homopellitorine (2) and 4 known compounds, namely pellitorine (1), pipyaqubine (3), 2-methylpropylamide (4), and N-isobutyl-2,4-eicosadienamide (5). Conclusion This first report on the larvicidal activity of P. nigrum extract and pure compounds on this tick species suggests that P. nigrum could be a natural biosourced alternative for the control of the larval stage of Rh. australis. Graphical Abstract |
| first_indexed | 2024-04-09T23:07:45Z |
| format | Article |
| id | doaj.art-b05f680c200149d98f1830f2c9ecf8cf |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-04-09T23:07:45Z |
| publishDate | 2023-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-b05f680c200149d98f1830f2c9ecf8cf2023-03-22T10:38:19ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412023-03-0110111110.1186/s40538-023-00389-5Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae)Marine Toussirot0Paul Coulerie1Thomas Hüe2Alexandre Maciuk3Valérie Kagy4Institut Agronomique néo-Calédonien, Equipe ARBOREAL (AgricultuRe BiOdiveRsité Et vALorisation)Institut Agronomique néo-Calédonien, Equipe ARBOREAL (AgricultuRe BiOdiveRsité Et vALorisation)Institut Agronomique néo-Calédonien, Equipe ARBOREAL (AgricultuRe BiOdiveRsité Et vALorisation)Université Paris-Saclay, CNRS, BioCISInstitut Agronomique néo-Calédonien, Equipe ARBOREAL (AgricultuRe BiOdiveRsité Et vALorisation)Abstract Background The cattle farming parasite Rhipicephalus australis is the main tick and one of the most important in the world from an economic point of view. Various studies have been developed in order to find plant extracts with effective acaricidal properties and environmentally friendly. Studies involving plant extracts for parasite control on commercial animal herds is a developing area in New Caledonia. Bioactive natural products play an important role as lead compounds in the development of new pesticides. Results The ethanolic extract of Piper nigrum L. dried fruits as well as the ethyl acetate extract and the methanolic extract of stems exhibited 100% larvicidal activity (50 mg/mL) against Rh. australis larvae, the cattle tick, an hematophagous parasite. Bioguided fractionation of the ethanolic extract of dried mature fruits using the same assay led to the isolation of five compounds belonging to piperamide family. The structures of isolated compounds were elucidated using spectroscopic methods: ESI-HRMS, 1H- and 13C-NMR spectral data, including DEPT and 2D-NMR experiments (COSY, HSQC, HMBC, and NOESY). These include 1 compound described for the first time in P. nigrum, homopellitorine (2) and 4 known compounds, namely pellitorine (1), pipyaqubine (3), 2-methylpropylamide (4), and N-isobutyl-2,4-eicosadienamide (5). Conclusion This first report on the larvicidal activity of P. nigrum extract and pure compounds on this tick species suggests that P. nigrum could be a natural biosourced alternative for the control of the larval stage of Rh. australis. Graphical Abstracthttps://doi.org/10.1186/s40538-023-00389-5PiperaceaePiper nigrum L.Plant extractsRhipicephalus australisCattle tickPiperamides |
| spellingShingle | Marine Toussirot Paul Coulerie Thomas Hüe Alexandre Maciuk Valérie Kagy Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae) Chemical and Biological Technologies in Agriculture Piperaceae Piper nigrum L. Plant extracts Rhipicephalus australis Cattle tick Piperamides |
| title | Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae) |
| title_full | Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae) |
| title_fullStr | Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae) |
| title_full_unstemmed | Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae) |
| title_short | Larvicidal activity of the black pepper, Piper nigrum (Fam: Piperaceae) extracts on the cattle tick, Rhipicephalus australis (Acari: Ixodidae) |
| title_sort | larvicidal activity of the black pepper piper nigrum fam piperaceae extracts on the cattle tick rhipicephalus australis acari ixodidae |
| topic | Piperaceae Piper nigrum L. Plant extracts Rhipicephalus australis Cattle tick Piperamides |
| url | https://doi.org/10.1186/s40538-023-00389-5 |
| work_keys_str_mv | AT marinetoussirot larvicidalactivityoftheblackpepperpipernigrumfampiperaceaeextractsonthecattletickrhipicephalusaustralisacariixodidae AT paulcoulerie larvicidalactivityoftheblackpepperpipernigrumfampiperaceaeextractsonthecattletickrhipicephalusaustralisacariixodidae AT thomashue larvicidalactivityoftheblackpepperpipernigrumfampiperaceaeextractsonthecattletickrhipicephalusaustralisacariixodidae AT alexandremaciuk larvicidalactivityoftheblackpepperpipernigrumfampiperaceaeextractsonthecattletickrhipicephalusaustralisacariixodidae AT valeriekagy larvicidalactivityoftheblackpepperpipernigrumfampiperaceaeextractsonthecattletickrhipicephalusaustralisacariixodidae |