RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices

A user-friendly, low-cost detector able to read the RGB indexes of microfluidic paper-based analytical devices (µPADs) was developed. The RGB-detector was built with 3D printing using PLA+ and reused Li-ion batteries. It is Arduino-based, which provides an easy interface between the sensor TCS3200,...

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Main Authors: Bianca Maria Pazzi, Dario Pistoia, Giancarla Alberti
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/13/10/1585
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author Bianca Maria Pazzi
Dario Pistoia
Giancarla Alberti
author_facet Bianca Maria Pazzi
Dario Pistoia
Giancarla Alberti
author_sort Bianca Maria Pazzi
collection DOAJ
description A user-friendly, low-cost detector able to read the RGB indexes of microfluidic paper-based analytical devices (µPADs) was developed. The RGB-detector was built with 3D printing using PLA+ and reused Li-ion batteries. It is Arduino-based, which provides an easy interface between the sensor TCS3200, which reads the quadratic wave of the times corresponding to the RGB numbers, the Arduino itself, whose software translates the times into RGB values, and the touchscreen display, NX3224T028, which shows the results. This detector permits multi-sample analysis since it has a sample holder that can keep up to six µPADs simultaneously and rotate after the display’s request. This work shows how the readings of the RGB indexes by the proposed RGB-detector implement the measurements’ reproducibility. As a proof-of-concept, the RGB-detector application to a green array of µPADs for pH measurement coupled with chemometric analysis allowed us to achieve good results in terms of precision and agreement with the pH values measured by a classical pH-meter.
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spelling doaj.art-b060405fd5a548de96932ae722359dba2023-11-24T01:21:02ZengMDPI AGMicromachines2072-666X2022-09-011310158510.3390/mi13101585RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical DevicesBianca Maria Pazzi0Dario Pistoia1Giancarla Alberti2Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, ItalyDepartment of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, ItalyDepartment of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, ItalyA user-friendly, low-cost detector able to read the RGB indexes of microfluidic paper-based analytical devices (µPADs) was developed. The RGB-detector was built with 3D printing using PLA+ and reused Li-ion batteries. It is Arduino-based, which provides an easy interface between the sensor TCS3200, which reads the quadratic wave of the times corresponding to the RGB numbers, the Arduino itself, whose software translates the times into RGB values, and the touchscreen display, NX3224T028, which shows the results. This detector permits multi-sample analysis since it has a sample holder that can keep up to six µPADs simultaneously and rotate after the display’s request. This work shows how the readings of the RGB indexes by the proposed RGB-detector implement the measurements’ reproducibility. As a proof-of-concept, the RGB-detector application to a green array of µPADs for pH measurement coupled with chemometric analysis allowed us to achieve good results in terms of precision and agreement with the pH values measured by a classical pH-meter.https://www.mdpi.com/2072-666X/13/10/1585RGB-detectorArduino-based devicelow-cost sensormicrofluidic paper-based analytical devicescolor analysis
spellingShingle Bianca Maria Pazzi
Dario Pistoia
Giancarla Alberti
RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices
Micromachines
RGB-detector
Arduino-based device
low-cost sensor
microfluidic paper-based analytical devices
color analysis
title RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices
title_full RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices
title_fullStr RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices
title_full_unstemmed RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices
title_short RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices
title_sort rgb detector a smart low cost device for reading rgb indexes of microfluidic paper based analytical devices
topic RGB-detector
Arduino-based device
low-cost sensor
microfluidic paper-based analytical devices
color analysis
url https://www.mdpi.com/2072-666X/13/10/1585
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AT giancarlaalberti rgbdetectorasmartlowcostdeviceforreadingrgbindexesofmicrofluidicpaperbasedanalyticaldevices