Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition
The interaction of soy protein isolate (SPI) and its hydrolysates (SPIHs) with cyanidin-3-<i>O</i>-glucoside (C3G) at pH 7.0 were investigated to clarify the changes in the antioxidant capacity of their complexes. The results of intrinsic fluorescence revealed that C3G binds to SPI/SPIHs...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/6/1721 |
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| author | Yaru Wu Zhucheng Yin Xuejiao Qie Yao Chen Maomao Zeng Zhaojun Wang Fang Qin Jie Chen Zhiyong He |
| author_facet | Yaru Wu Zhucheng Yin Xuejiao Qie Yao Chen Maomao Zeng Zhaojun Wang Fang Qin Jie Chen Zhiyong He |
| author_sort | Yaru Wu |
| collection | DOAJ |
| description | The interaction of soy protein isolate (SPI) and its hydrolysates (SPIHs) with cyanidin-3-<i>O</i>-glucoside (C3G) at pH 7.0 were investigated to clarify the changes in the antioxidant capacity of their complexes. The results of intrinsic fluorescence revealed that C3G binds to SPI/SPIHs mainly through hydrophobic interaction, and the binding affinity of SPI was stronger than that of SPIHs. Circular dichroism and Fourier-transform infrared spectroscopy analyses revealed that the interaction with C3G did not significantly change the secondary structures of SPI/SPIHs, while the surface hydrophobicity and average particle size of proteins decreased. Furthermore, the SPI/SPIHs-C3G interaction induced an antagonistic effect on the antioxidant capacity (ABTS and DPPH) of the complex system, with the masking effect on the ABTS scavenging capacity of the SPIHs-C3G complexes being lower than that of the SPI-C3G complexes. This study contributes to the design and development of functional beverages that are rich in hydrolysates and anthocyanins. |
| first_indexed | 2024-03-10T13:05:08Z |
| format | Article |
| id | doaj.art-b06449bf347c4608981cfbef29636e05 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:05:08Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b06449bf347c4608981cfbef29636e052023-11-21T11:12:38ZengMDPI AGMolecules1420-30492021-03-01266172110.3390/molecules26061721Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral ConditionYaru Wu0Zhucheng Yin1Xuejiao Qie2Yao Chen3Maomao Zeng4Zhaojun Wang5Fang Qin6Jie Chen7Zhiyong He8State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, ChinaThe interaction of soy protein isolate (SPI) and its hydrolysates (SPIHs) with cyanidin-3-<i>O</i>-glucoside (C3G) at pH 7.0 were investigated to clarify the changes in the antioxidant capacity of their complexes. The results of intrinsic fluorescence revealed that C3G binds to SPI/SPIHs mainly through hydrophobic interaction, and the binding affinity of SPI was stronger than that of SPIHs. Circular dichroism and Fourier-transform infrared spectroscopy analyses revealed that the interaction with C3G did not significantly change the secondary structures of SPI/SPIHs, while the surface hydrophobicity and average particle size of proteins decreased. Furthermore, the SPI/SPIHs-C3G interaction induced an antagonistic effect on the antioxidant capacity (ABTS and DPPH) of the complex system, with the masking effect on the ABTS scavenging capacity of the SPIHs-C3G complexes being lower than that of the SPI-C3G complexes. This study contributes to the design and development of functional beverages that are rich in hydrolysates and anthocyanins.https://www.mdpi.com/1420-3049/26/6/1721soy protein isolatehydrolysatecyanidin-3-<i>O</i>-glucosidenoncovalent interactionantioxidant capacity |
| spellingShingle | Yaru Wu Zhucheng Yin Xuejiao Qie Yao Chen Maomao Zeng Zhaojun Wang Fang Qin Jie Chen Zhiyong He Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition Molecules soy protein isolate hydrolysate cyanidin-3-<i>O</i>-glucoside noncovalent interaction antioxidant capacity |
| title | Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition |
| title_full | Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition |
| title_fullStr | Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition |
| title_full_unstemmed | Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition |
| title_short | Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-<i>O</i>-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition |
| title_sort | interaction of soy protein isolate hydrolysates with cyanidin 3 i o i glucoside and its effect on the in vitro antioxidant capacity of the complexes under neutral condition |
| topic | soy protein isolate hydrolysate cyanidin-3-<i>O</i>-glucoside noncovalent interaction antioxidant capacity |
| url | https://www.mdpi.com/1420-3049/26/6/1721 |
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