Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs
Objective(s): Gallium-68 DOTA-DPhe1-Tyr3-Octreotide (68Ga-DOTATOC) has been applied by several European centers for the treatment of a variety of human malignancies. Nevertheless, definitive dosimetric data are yet unavailable. According to the Society of Nuclear Medicine and Molecular Imaging, rese...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-01-01
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| Series: | Asia Oceania Journal of Nuclear Medicine and Biology |
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| Online Access: | http://aojnmb.mums.ac.ir/pdf_5043_76a2ad14878b96963330ff68c4e18f99.html |
| _version_ | 1818528715441700864 |
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| author | mojdeh naderi samaneh zolghadri hassan yousefnia ali ramazani amir reza jalilian |
| author_facet | mojdeh naderi samaneh zolghadri hassan yousefnia ali ramazani amir reza jalilian |
| author_sort | mojdeh naderi |
| collection | DOAJ |
| description | Objective(s): Gallium-68 DOTA-DPhe1-Tyr3-Octreotide (68Ga-DOTATOC) has been applied by several European centers for the treatment of a variety of human malignancies. Nevertheless, definitive dosimetric data are yet unavailable. According to the Society of Nuclear Medicine and Molecular Imaging, researchers are investigating the safety and efficacy of this radiotracer to meet Food and Drug Administration requirements. The aim of this study was to introduce the optimized procedure for 68Ga-DOTATOC preparation, using a novel germanium-68 (68Ge)/68Ga generator in Iran and evaluate the absorbed doses in numerous organs with high accuracy. Methods: The optimized conditions for preparing the radiolabeled complex were determined via several experiments by changing the ligand concentration, pH, temperature and incubation time. Radiochemical purity of the complex was assessed, using high-performance liquid chromatography and instant thin-layer chromatography. The absorbed dose of human organs was evaluated, based on biodistribution studies on Syrian rats via Radiation Absorbed Dose Assessment Resource Method. Results: 68Ga-DOTATOC was prepared with radiochemical purity of >98% and specific activity of 39.6 MBq/nmol. The complex demonstrated great stability at room temperature and in human serum at 37°C at least two hours after preparation. Significant uptake was observed in somatostatin receptor-positive tissues such as pancreatic and adrenal tissues (12.83 %ID/g and 0.91 %ID/g, respectively). Dose estimations in human organs showed that the pancreas, kidneys and adrenal glands received the maximum absorbed doses (0.105, 0.074 and 0.010 mGy/MBq, respectively). Also, the effective absorbed dose was estimated at 0.026 mSv/MBq for 68Ga-DOTATOC. Conclusion: The obtained results showed that 68Ga-DOTATOC can be considered as an effective agent for clinical PET imaging in Iran. |
| first_indexed | 2024-12-11T06:53:36Z |
| format | Article |
| id | doaj.art-b0657298ef334b34aa9b0aea249a763a |
| institution | Directory Open Access Journal |
| issn | 2322-5718 2322-5726 |
| language | English |
| last_indexed | 2024-12-11T06:53:36Z |
| publishDate | 2016-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Asia Oceania Journal of Nuclear Medicine and Biology |
| spelling | doaj.art-b0657298ef334b34aa9b0aea249a763a2022-12-22T01:16:49ZengMashhad University of Medical SciencesAsia Oceania Journal of Nuclear Medicine and Biology2322-57182322-57262016-01-0141192910.7508/aojnmb.2016.04.0045043Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organsmojdeh naderi0samaneh zolghadri1hassan yousefnia2ali ramazani3amir reza jalilian4Department of Chemistry, University of Zanjan, Zanjan, IranNuclear Science and Technology Research Institute (NSTRI), Tehran, IranNuclear Science and Technology Research Institute (NSTRI), Tehran, IranDepartment of Chemistry, University of Zanjan, Zanjan, IranNuclear Science and Technology Research Institute (NSTRI), Tehran, IranObjective(s): Gallium-68 DOTA-DPhe1-Tyr3-Octreotide (68Ga-DOTATOC) has been applied by several European centers for the treatment of a variety of human malignancies. Nevertheless, definitive dosimetric data are yet unavailable. According to the Society of Nuclear Medicine and Molecular Imaging, researchers are investigating the safety and efficacy of this radiotracer to meet Food and Drug Administration requirements. The aim of this study was to introduce the optimized procedure for 68Ga-DOTATOC preparation, using a novel germanium-68 (68Ge)/68Ga generator in Iran and evaluate the absorbed doses in numerous organs with high accuracy. Methods: The optimized conditions for preparing the radiolabeled complex were determined via several experiments by changing the ligand concentration, pH, temperature and incubation time. Radiochemical purity of the complex was assessed, using high-performance liquid chromatography and instant thin-layer chromatography. The absorbed dose of human organs was evaluated, based on biodistribution studies on Syrian rats via Radiation Absorbed Dose Assessment Resource Method. Results: 68Ga-DOTATOC was prepared with radiochemical purity of >98% and specific activity of 39.6 MBq/nmol. The complex demonstrated great stability at room temperature and in human serum at 37°C at least two hours after preparation. Significant uptake was observed in somatostatin receptor-positive tissues such as pancreatic and adrenal tissues (12.83 %ID/g and 0.91 %ID/g, respectively). Dose estimations in human organs showed that the pancreas, kidneys and adrenal glands received the maximum absorbed doses (0.105, 0.074 and 0.010 mGy/MBq, respectively). Also, the effective absorbed dose was estimated at 0.026 mSv/MBq for 68Ga-DOTATOC. Conclusion: The obtained results showed that 68Ga-DOTATOC can be considered as an effective agent for clinical PET imaging in Iran.http://aojnmb.mums.ac.ir/pdf_5043_76a2ad14878b96963330ff68c4e18f99.htmlGa-68OctreotideInternal DosimetrySomatostatin |
| spellingShingle | mojdeh naderi samaneh zolghadri hassan yousefnia ali ramazani amir reza jalilian Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs Asia Oceania Journal of Nuclear Medicine and Biology Ga-68 Octreotide Internal Dosimetry Somatostatin |
| title | Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs |
| title_full | Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs |
| title_fullStr | Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs |
| title_full_unstemmed | Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs |
| title_short | Preclinical Studies of 68Ga-DOTATOC: Biodistribution Assessment in Syrian Rats and Evaluation of Absorbed Dose in Human Organs |
| title_sort | preclinical studies of 68ga dotatoc biodistribution assessment in syrian rats and evaluation of absorbed dose in human organs |
| topic | Ga-68 Octreotide Internal Dosimetry Somatostatin |
| url | http://aojnmb.mums.ac.ir/pdf_5043_76a2ad14878b96963330ff68c4e18f99.html |
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