CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00240/full |
| _version_ | 1818363034131759104 |
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| author | Bhaskar Roy Jing Zhao Jing Zhao Chao Yang Wen Luo Teng Xiong Yong Li Xiaodong Fang Guanjun Gao Chabungbam O. Singh Lise Madsen Lise Madsen Lise Madsen Yong Zhou Karsten Kristiansen Karsten Kristiansen |
| author_facet | Bhaskar Roy Jing Zhao Jing Zhao Chao Yang Wen Luo Teng Xiong Yong Li Xiaodong Fang Guanjun Gao Chabungbam O. Singh Lise Madsen Lise Madsen Lise Madsen Yong Zhou Karsten Kristiansen Karsten Kristiansen |
| author_sort | Bhaskar Roy |
| collection | DOAJ |
| description | Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field. |
| first_indexed | 2024-12-13T21:42:03Z |
| format | Article |
| id | doaj.art-b06591309fb7473aab11fc40063feeb3 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T21:42:03Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-b06591309fb7473aab11fc40063feeb32022-12-21T23:30:30ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-07-01910.3389/fgene.2018.00240388429CRISPR/Cascade 9-Mediated Genome Editing-Challenges and OpportunitiesBhaskar Roy0Jing Zhao1Jing Zhao2Chao Yang3Wen Luo4Teng Xiong5Yong Li6Xiaodong Fang7Guanjun Gao8Chabungbam O. Singh9Lise Madsen10Lise Madsen11Lise Madsen12Yong Zhou13Karsten Kristiansen14Karsten Kristiansen15BGI Genomics, BGI-Shenzhen, Shenzhen, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, DenmarkBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaSchool of Life Sciences, Tsinghua University, Beijing, ChinaInstitute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, DenmarkInstitute of Marine Research, Bergen, NorwayBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen, ChinaLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, DenmarkClustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field.https://www.frontiersin.org/article/10.3389/fgene.2018.00240/fullCRISPRCascade 9genome editingoff-target effectsgene targetingRNA guided system |
| spellingShingle | Bhaskar Roy Jing Zhao Jing Zhao Chao Yang Wen Luo Teng Xiong Yong Li Xiaodong Fang Guanjun Gao Chabungbam O. Singh Lise Madsen Lise Madsen Lise Madsen Yong Zhou Karsten Kristiansen Karsten Kristiansen CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities Frontiers in Genetics CRISPR Cascade 9 genome editing off-target effects gene targeting RNA guided system |
| title | CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities |
| title_full | CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities |
| title_fullStr | CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities |
| title_full_unstemmed | CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities |
| title_short | CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities |
| title_sort | crispr cascade 9 mediated genome editing challenges and opportunities |
| topic | CRISPR Cascade 9 genome editing off-target effects gene targeting RNA guided system |
| url | https://www.frontiersin.org/article/10.3389/fgene.2018.00240/full |
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