Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains
ObjectiveToday, the emergence of Klebsiella pneumoniae with the tmexCD1-toprJ1 gene cassette in patients has presented a significant clinical challenge.MethodsTo present the detailed genetic features of the tmexCD1-toprJ1 gene cassette of K. pneumoniae strain F4_plasmid pA, the whole bacterial genom...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1260066/full |
| _version_ | 1797660666447790080 |
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| author | Ying Qu Wenji Wang Qinhong Lu Jihai Qiu Dongguo Wang Liman Ma Liman Ma |
| author_facet | Ying Qu Wenji Wang Qinhong Lu Jihai Qiu Dongguo Wang Liman Ma Liman Ma |
| author_sort | Ying Qu |
| collection | DOAJ |
| description | ObjectiveToday, the emergence of Klebsiella pneumoniae with the tmexCD1-toprJ1 gene cassette in patients has presented a significant clinical challenge.MethodsTo present the detailed genetic features of the tmexCD1-toprJ1 gene cassette of K. pneumoniae strain F4_plasmid pA, the whole bacterial genome was sequenced by Illumina and nanopore platforms, and mobile genetic elements related to antibiotic resistance genes were analyzed with a series of bioinformatics methods.ResultsK. pneumoniae strain F4 was determined to be a class A+C beta-lactamase, and was resistant to routinely used antibiotics, especially tigecycline, because of the oqxAB gene localized on the F4_chromosome and tmexCD1-toprJ1 on F4_plasmid A. After plasmid transfer assays, the F4_plasmid pA or F4_plasmid pB could be recovered with an average conjugation frequencies of 3.42×10-4 or 4.19×10-4. F4_plasmid pA carried tmexCD1-toprJ1 and blaDHA-1 accompanied by genetic intermixing of TnAs1, Tn5393, TnAs3, and In641, while F4_plasmid pB, bearing blaCTX-M-174, had structural overlap of TnAs3 and In641. ConclusionsWe suggested that plasmids carrying tmexCD1- toprJ1 might be strongly related to IS26-integrated loop intermediates. This study showed that due to the structural evolution of F4 and related strains, their resistances were so strong that effective antibiotics were virtually unavailable, therefore their spread and prevalence should be strictly controlled. |
| first_indexed | 2024-03-11T18:34:13Z |
| format | Article |
| id | doaj.art-b067efe915ca422796f1f428f284ab16 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T18:34:13Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-b067efe915ca422796f1f428f284ab162023-10-13T07:33:27ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-10-011310.3389/fcimb.2023.12600661260066Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strainsYing Qu0Wenji Wang1Qinhong Lu2Jihai Qiu3Dongguo Wang4Liman Ma5Liman Ma6Department of Clinical Medicine Laboratory, Taizhou Municipal Hospital Affiliated with Taizhou University, Taizhou, Zhejiang, ChinaSchool of Life Sciences, Taizhou University, Taizhou, Zhejiang, ChinaDepartment of Clinical Medicine Laboratory, Ningbo Medical Center Li Huili Hospital, Ningbo, Zhejiang, ChinaDepartment of Infectious Diseases, Taizhou Municipal Hospital Affiliated with Taizhou University, Taizhou, Zhejiang, ChinaDepartment of Central Laboratory, Taizhou Municipal Hospital Affiliated with Taizhou University, Taizhou, Zhejiang, ChinaDepartment of Central Laboratory, Taizhou Municipal Hospital Affiliated with Taizhou University, Taizhou, Zhejiang, ChinaSchool of Medicine, Taizhou University, Taizhou, Zhejiang, ChinaObjectiveToday, the emergence of Klebsiella pneumoniae with the tmexCD1-toprJ1 gene cassette in patients has presented a significant clinical challenge.MethodsTo present the detailed genetic features of the tmexCD1-toprJ1 gene cassette of K. pneumoniae strain F4_plasmid pA, the whole bacterial genome was sequenced by Illumina and nanopore platforms, and mobile genetic elements related to antibiotic resistance genes were analyzed with a series of bioinformatics methods.ResultsK. pneumoniae strain F4 was determined to be a class A+C beta-lactamase, and was resistant to routinely used antibiotics, especially tigecycline, because of the oqxAB gene localized on the F4_chromosome and tmexCD1-toprJ1 on F4_plasmid A. After plasmid transfer assays, the F4_plasmid pA or F4_plasmid pB could be recovered with an average conjugation frequencies of 3.42×10-4 or 4.19×10-4. F4_plasmid pA carried tmexCD1-toprJ1 and blaDHA-1 accompanied by genetic intermixing of TnAs1, Tn5393, TnAs3, and In641, while F4_plasmid pB, bearing blaCTX-M-174, had structural overlap of TnAs3 and In641. ConclusionsWe suggested that plasmids carrying tmexCD1- toprJ1 might be strongly related to IS26-integrated loop intermediates. This study showed that due to the structural evolution of F4 and related strains, their resistances were so strong that effective antibiotics were virtually unavailable, therefore their spread and prevalence should be strictly controlled.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1260066/fullK. pneumoniaeF4_plasmid pAF4_plasmid pBtigecycline resistancemultidrug resistancetmexCD1-toprJ1 |
| spellingShingle | Ying Qu Wenji Wang Qinhong Lu Jihai Qiu Dongguo Wang Liman Ma Liman Ma Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains Frontiers in Cellular and Infection Microbiology K. pneumoniae F4_plasmid pA F4_plasmid pB tigecycline resistance multidrug resistance tmexCD1-toprJ1 |
| title | Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains |
| title_full | Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains |
| title_fullStr | Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains |
| title_full_unstemmed | Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains |
| title_short | Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, blaDHA-1, and blaCTX-M-127, in clinical Klebsiella pneumoniae strains |
| title_sort | occurrence and characterization of plasmids carrying tmexcd1 toprj1 bladha 1 and blactx m 127 in clinical klebsiella pneumoniae strains |
| topic | K. pneumoniae F4_plasmid pA F4_plasmid pB tigecycline resistance multidrug resistance tmexCD1-toprJ1 |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1260066/full |
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