Cloning and expression analysis of Rubisco activase genes in Carya cathayensis
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a key enzyme involved in CO2 assimilation during photosynthesis. Rubisco activation depends on the activity of Rubisco activase (RCA). We performed 3′/5′ rapid amplification of cDNA ends (RACE) and reverse transcription polymerase chain re...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-09-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1208060 |
| _version_ | 1828532672184451072 |
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| author | Guo Cun Ji Bing Song Zheng Xue Qin Li Xiang Tao Zhu Song Heng Jin |
| author_facet | Guo Cun Ji Bing Song Zheng Xue Qin Li Xiang Tao Zhu Song Heng Jin |
| author_sort | Guo Cun Ji |
| collection | DOAJ |
| description | Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a key enzyme involved in CO2 assimilation during photosynthesis. Rubisco activation depends on the activity of Rubisco activase (RCA). We performed 3′/5′ rapid amplification of cDNA ends (RACE) and reverse transcription polymerase chain reaction (RT-PCR) to amplify the 3′ and 5′ end sequences of RCA genes from hickory. We obtained two full-length gene sequences, designated CcRCAα and CcRCAβ. The two corresponding cDNAs are divergent in both the translated and 3′ untranslated regions. The analysis of the genomic DNA sequences suggested that the corresponding mRNAs are transcripts of two different genes and not the products of a single alternatively spliced pre-mRNA. We examined the expression of CcRCAα and CcRCAβ in hickory leaves at various stages of development by quantitative real-time PCR (qRT-PCR) analysis. The results suggest that RCA genes play an important role in development and environmental responses. These results provide a basis for modulating RCA gene expression to improve the photosynthetic rate and plant growth in hickory. |
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| format | Article |
| id | doaj.art-b06987a72b7f497c8f4b72c1093ef0a1 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-11T22:56:30Z |
| publishDate | 2016-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-b06987a72b7f497c8f4b72c1093ef0a12022-12-22T00:47:13ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-09-0130583484110.1080/13102818.2016.12080601208060Cloning and expression analysis of Rubisco activase genes in Carya cathayensisGuo Cun Ji0Bing Song Zheng1Xue Qin Li2Xiang Tao Zhu3Song Heng Jin4Zhejiang A & F UniversityZhejiang A & F UniversityZhejiang A & F UniversityZhejiang A & F UniversityZhejiang A & F UniversityRibulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a key enzyme involved in CO2 assimilation during photosynthesis. Rubisco activation depends on the activity of Rubisco activase (RCA). We performed 3′/5′ rapid amplification of cDNA ends (RACE) and reverse transcription polymerase chain reaction (RT-PCR) to amplify the 3′ and 5′ end sequences of RCA genes from hickory. We obtained two full-length gene sequences, designated CcRCAα and CcRCAβ. The two corresponding cDNAs are divergent in both the translated and 3′ untranslated regions. The analysis of the genomic DNA sequences suggested that the corresponding mRNAs are transcripts of two different genes and not the products of a single alternatively spliced pre-mRNA. We examined the expression of CcRCAα and CcRCAβ in hickory leaves at various stages of development by quantitative real-time PCR (qRT-PCR) analysis. The results suggest that RCA genes play an important role in development and environmental responses. These results provide a basis for modulating RCA gene expression to improve the photosynthetic rate and plant growth in hickory.http://dx.doi.org/10.1080/13102818.2016.1208060Carya cathayensisRubisco activasecloninggene expression |
| spellingShingle | Guo Cun Ji Bing Song Zheng Xue Qin Li Xiang Tao Zhu Song Heng Jin Cloning and expression analysis of Rubisco activase genes in Carya cathayensis Biotechnology & Biotechnological Equipment Carya cathayensis Rubisco activase cloning gene expression |
| title | Cloning and expression analysis of Rubisco activase genes in Carya cathayensis |
| title_full | Cloning and expression analysis of Rubisco activase genes in Carya cathayensis |
| title_fullStr | Cloning and expression analysis of Rubisco activase genes in Carya cathayensis |
| title_full_unstemmed | Cloning and expression analysis of Rubisco activase genes in Carya cathayensis |
| title_short | Cloning and expression analysis of Rubisco activase genes in Carya cathayensis |
| title_sort | cloning and expression analysis of rubisco activase genes in carya cathayensis |
| topic | Carya cathayensis Rubisco activase cloning gene expression |
| url | http://dx.doi.org/10.1080/13102818.2016.1208060 |
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