Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.)
Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by geneti...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Genetics and Agricultural Biotechnology Institute of Tabarestan
2014-06-01
|
| Series: | Journal of Plant Molecular Breeding |
| Subjects: | |
| Online Access: | http://www.jpmb-gabit.ir/article_8426_9d7968656f4966d33024fc319ec29b83.pdf |
| _version_ | 1818969891934306304 |
|---|---|
| author | Mahdieh Yousefiara Maryam Jafarkhani Kermani Abdolreza Bagheri Aliakbar Habashi Hamid Abdollahi |
| author_facet | Mahdieh Yousefiara Maryam Jafarkhani Kermani Abdolreza Bagheri Aliakbar Habashi Hamid Abdollahi |
| author_sort | Mahdieh Yousefiara |
| collection | DOAJ |
| description | Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. A general prerequisite for these approaches is to establish an efficient plant regeneration system. In the present study, the effect of two basal media (MS and NN) and different concentrations of TDZ (0, 2.5, 5, 7.5 μM) or BAP (0, 4, 8, 16 μM) in combination with NAA (1 μM) on direct shoot regeneration of two pear (<em>Pyrus communis</em> L.) genotypes 'Bartlett' and 'Dargazi' was investigated. The obtained results showed that 'Dargazi' had higher rates of shoot regeneration than 'Bartlett' and in both cultivars the highest percent of shoot regeneration was observed from lower sections of the leaves. Although the highest percent of shoot regeneration in 'Bartlett' (38%) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA, the differences in shoot regeneration between this medium and the NN media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant. The highest percent of shoot regeneration in 'Dargazi' (56%) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA. It can be concluded that genotypes, explant types and culture media composition could effect on direct shoot regeneration of pear. |
| first_indexed | 2024-12-20T14:27:48Z |
| format | Article |
| id | doaj.art-b06a29d4e8b64184bae7303ab27e231b |
| institution | Directory Open Access Journal |
| issn | 2322-3332 2322-5092 |
| language | English |
| last_indexed | 2024-12-20T14:27:48Z |
| publishDate | 2014-06-01 |
| publisher | Genetics and Agricultural Biotechnology Institute of Tabarestan |
| record_format | Article |
| series | Journal of Plant Molecular Breeding |
| spelling | doaj.art-b06a29d4e8b64184bae7303ab27e231b2022-12-21T19:37:43ZengGenetics and Agricultural Biotechnology Institute of TabarestanJournal of Plant Molecular Breeding2322-33322322-50922014-06-0121212810.22058/jpmb.2014.84268426Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.)Mahdieh Yousefiara0Maryam Jafarkhani Kermani1Abdolreza Bagheri2Aliakbar Habashi3Hamid Abdollahi4Department of Biotechnology and Plant Breeding, Faculty of Agriculture , Ferdowsi University of Mashhad.Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII).Department of Biotechnology and Plant Breeding, Faculty of Agriculture , Ferdowsi University of Mashhad.Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII).Department of Horticultural Research, Seed and Plant Improvement Institute, Karaj, Iran.Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. A general prerequisite for these approaches is to establish an efficient plant regeneration system. In the present study, the effect of two basal media (MS and NN) and different concentrations of TDZ (0, 2.5, 5, 7.5 μM) or BAP (0, 4, 8, 16 μM) in combination with NAA (1 μM) on direct shoot regeneration of two pear (<em>Pyrus communis</em> L.) genotypes 'Bartlett' and 'Dargazi' was investigated. The obtained results showed that 'Dargazi' had higher rates of shoot regeneration than 'Bartlett' and in both cultivars the highest percent of shoot regeneration was observed from lower sections of the leaves. Although the highest percent of shoot regeneration in 'Bartlett' (38%) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA, the differences in shoot regeneration between this medium and the NN media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant. The highest percent of shoot regeneration in 'Dargazi' (56%) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA. It can be concluded that genotypes, explant types and culture media composition could effect on direct shoot regeneration of pear.http://www.jpmb-gabit.ir/article_8426_9d7968656f4966d33024fc319ec29b83.pdfdirect shoot regenerationpearthidiazuron |
| spellingShingle | Mahdieh Yousefiara Maryam Jafarkhani Kermani Abdolreza Bagheri Aliakbar Habashi Hamid Abdollahi Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.) Journal of Plant Molecular Breeding direct shoot regeneration pear thidiazuron |
| title | Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.) |
| title_full | Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.) |
| title_fullStr | Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.) |
| title_full_unstemmed | Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.) |
| title_short | Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.) |
| title_sort | study of factors affecting direct shoot regeneration of pear pyrus communis l |
| topic | direct shoot regeneration pear thidiazuron |
| url | http://www.jpmb-gabit.ir/article_8426_9d7968656f4966d33024fc319ec29b83.pdf |
| work_keys_str_mv | AT mahdiehyousefiara studyoffactorsaffectingdirectshootregenerationofpearpyruscommunisl AT maryamjafarkhanikermani studyoffactorsaffectingdirectshootregenerationofpearpyruscommunisl AT abdolrezabagheri studyoffactorsaffectingdirectshootregenerationofpearpyruscommunisl AT aliakbarhabashi studyoffactorsaffectingdirectshootregenerationofpearpyruscommunisl AT hamidabdollahi studyoffactorsaffectingdirectshootregenerationofpearpyruscommunisl |