Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator.
In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3384643?pdf=render |
| _version_ | 1828192935079837696 |
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| author | Yujia Liu Yichen Ding Eric Alonas Wenli Zhao Philip J Santangelo Dayong Jin James A Piper Junlin Teng Qiushi Ren Peng Xi |
| author_facet | Yujia Liu Yichen Ding Eric Alonas Wenli Zhao Philip J Santangelo Dayong Jin James A Piper Junlin Teng Qiushi Ren Peng Xi |
| author_sort | Yujia Liu |
| collection | DOAJ |
| description | In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED. |
| first_indexed | 2024-04-12T09:01:51Z |
| format | Article |
| id | doaj.art-b06d9abcb57245f0b83e212c0a238897 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T09:01:51Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b06d9abcb57245f0b83e212c0a2388972022-12-22T03:39:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e4000310.1371/journal.pone.0040003Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator.Yujia LiuYichen DingEric AlonasWenli ZhaoPhilip J SantangeloDayong JinJames A PiperJunlin TengQiushi RenPeng XiIn this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED.http://europepmc.org/articles/PMC3384643?pdf=render |
| spellingShingle | Yujia Liu Yichen Ding Eric Alonas Wenli Zhao Philip J Santangelo Dayong Jin James A Piper Junlin Teng Qiushi Ren Peng Xi Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. PLoS ONE |
| title | Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. |
| title_full | Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. |
| title_fullStr | Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. |
| title_full_unstemmed | Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. |
| title_short | Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. |
| title_sort | achieving λ 10 resolution cw sted nanoscopy with a ti sapphire oscillator |
| url | http://europepmc.org/articles/PMC3384643?pdf=render |
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