Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering
3D nanocomposite scaffolds have attracted significant attention in bone tissue engineering applications. In the current study, we fabricated a 3D nanocomposite scaffold based on a bacterial polyglucuronic acid (PGU) and sodium alginate (Alg) composite with carbon nanofibers (CNFs) as the bone tissue...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/7/2494 |
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| author | Zahra Ebrahimvand Dibazar Mahnaz Mohammadpour Hadi Samadian Soheila Zare Mehdi Azizi Masoud Hamidi Redouan Elboutachfaiti Emmanuel Petit Cédric Delattre |
| author_facet | Zahra Ebrahimvand Dibazar Mahnaz Mohammadpour Hadi Samadian Soheila Zare Mehdi Azizi Masoud Hamidi Redouan Elboutachfaiti Emmanuel Petit Cédric Delattre |
| author_sort | Zahra Ebrahimvand Dibazar |
| collection | DOAJ |
| description | 3D nanocomposite scaffolds have attracted significant attention in bone tissue engineering applications. In the current study, we fabricated a 3D nanocomposite scaffold based on a bacterial polyglucuronic acid (PGU) and sodium alginate (Alg) composite with carbon nanofibers (CNFs) as the bone tissue engineering scaffold. The CNFs were obtained from electrospun polyacrylonitrile nanofibers through heat treatment. The fabricated CNFs were incorporated into a PGU/Alg polymeric solution, which was physically cross-linked using CaCl<sub>2</sub> solution. The fabricated nanocomposites were characterized to evaluate the internal structure, porosity, swelling kinetics, hemocompatibility, and cytocompatibility. The characterizations indicated that the nanocomposites have a porous structure with interconnected pores architecture, proper water absorption, and retention characteristics. The in vitro studies revealed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability assessment showed that the nanocomposites were biocompatible and supported bone cell growth. These results indicated that the fabricated bacterial PGU/Alg/CNFs hydrogel nanocomposite exhibited appropriate properties and can be considered a new biomaterial for bone tissue engineering scaffolds. |
| first_indexed | 2024-03-09T11:40:30Z |
| format | Article |
| id | doaj.art-b070315988b643b7b71eed80505f1cb4 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T11:40:30Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-b070315988b643b7b71eed80505f1cb42023-11-30T23:32:55ZengMDPI AGMaterials1996-19442022-03-01157249410.3390/ma15072494Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue EngineeringZahra Ebrahimvand Dibazar0Mahnaz Mohammadpour1Hadi Samadian2Soheila Zare3Mehdi Azizi4Masoud Hamidi5Redouan Elboutachfaiti6Emmanuel Petit7Cédric Delattre8Department of Oral and Maxillo Facial Medicine, Faculty of Dentistry, Tabriz Asad University of Medical Sciences, Tabriz 5166616471, IranDepartment of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran 1411713116, IranPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah 6715847141, IranStudent Research Committee, Zanjan University of Medical Sciences, Zanjan 7797845157, IranDepartment of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan 6517838636, IranBioMatter-Biomass Transformation Lab (BTL), École Polytechnique de Bruxelles, Université Libre de Bruxelles, Avenue F.D. Roosevelt, 50-CP 165/61, 1050 Brussels, BelgiumUMRT INRAE 1158 BioEcoAgro, Laboratoire BIOPI, Université de Picardie Jules Verne, IUT d’Amiens, 80025 Amiens, FranceUMRT INRAE 1158 BioEcoAgro, Laboratoire BIOPI, Université de Picardie Jules Verne, IUT d’Amiens, 80025 Amiens, FranceUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, 63000 Clermont-Ferrand, France3D nanocomposite scaffolds have attracted significant attention in bone tissue engineering applications. In the current study, we fabricated a 3D nanocomposite scaffold based on a bacterial polyglucuronic acid (PGU) and sodium alginate (Alg) composite with carbon nanofibers (CNFs) as the bone tissue engineering scaffold. The CNFs were obtained from electrospun polyacrylonitrile nanofibers through heat treatment. The fabricated CNFs were incorporated into a PGU/Alg polymeric solution, which was physically cross-linked using CaCl<sub>2</sub> solution. The fabricated nanocomposites were characterized to evaluate the internal structure, porosity, swelling kinetics, hemocompatibility, and cytocompatibility. The characterizations indicated that the nanocomposites have a porous structure with interconnected pores architecture, proper water absorption, and retention characteristics. The in vitro studies revealed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability assessment showed that the nanocomposites were biocompatible and supported bone cell growth. These results indicated that the fabricated bacterial PGU/Alg/CNFs hydrogel nanocomposite exhibited appropriate properties and can be considered a new biomaterial for bone tissue engineering scaffolds.https://www.mdpi.com/1996-1944/15/7/2494polyglucuronic acid<i>Sinorhizobium meliloti</i> M5N1CScarbon nanofibershydrogelbone tissue engineeringnanocomposites |
| spellingShingle | Zahra Ebrahimvand Dibazar Mahnaz Mohammadpour Hadi Samadian Soheila Zare Mehdi Azizi Masoud Hamidi Redouan Elboutachfaiti Emmanuel Petit Cédric Delattre Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering Materials polyglucuronic acid <i>Sinorhizobium meliloti</i> M5N1CS carbon nanofibers hydrogel bone tissue engineering nanocomposites |
| title | Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering |
| title_full | Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering |
| title_fullStr | Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering |
| title_full_unstemmed | Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering |
| title_short | Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering |
| title_sort | bacterial polyglucuronic acid alginate carbon nanofibers hydrogel nanocomposite as a potential scaffold for bone tissue engineering |
| topic | polyglucuronic acid <i>Sinorhizobium meliloti</i> M5N1CS carbon nanofibers hydrogel bone tissue engineering nanocomposites |
| url | https://www.mdpi.com/1996-1944/15/7/2494 |
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