Reducing the Number of Mismatches between Hairs and Buccal References When Analysing mtDNA Heteroplasmic Variation by Massively Parallel Sequencing

Reducing the Number of Mismatches between Hairs and Buccal References When Analysing mtDNA Heteroplasmic Variation by Massively Parallel Sequencing

In forensics, mitochondrial DNA (mtDNA) analysis is foremost applied to rootless hairs often lacking detectable nuclear DNA. Sanger sequencing is the routine mtDNA method in most forensic laboratories, even though interpretation of mixed samples and heteroplasmic sites can be challenging. Individual...

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Bibliographic Details
Main Authors: Kristiaan J. van der Gaag, Stijn Desmyter, Sophie Smit, Lourdes Prieto, Titia Sijen
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Genes
Subjects:
MPS
control region
heteroplasmy
mitochondrial
Sanger
detection threshold
Online Access:https://www.mdpi.com/2073-4425/11/11/1355

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