Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier
Polymeric nanoparticles are one method to modify the drug release of small hydrophilic molecules. In this study, clindamycin HCl was used as a model drug loaded in carboxymethyl chitosan nanoparticles cross-linked with Ca<sup>2+</sup> ions (CMCS-Ca<sup>2+</sup>). The ultrason...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/9/1736 |
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| author | Tanpong Chaiwarit Sarana Rose Sommano Pornchai Rachtanapun Nutthapong Kantrong Warintorn Ruksiriwanich Mont Kumpugdee-Vollrath Pensak Jantrawut |
| author_facet | Tanpong Chaiwarit Sarana Rose Sommano Pornchai Rachtanapun Nutthapong Kantrong Warintorn Ruksiriwanich Mont Kumpugdee-Vollrath Pensak Jantrawut |
| author_sort | Tanpong Chaiwarit |
| collection | DOAJ |
| description | Polymeric nanoparticles are one method to modify the drug release of small hydrophilic molecules. In this study, clindamycin HCl was used as a model drug loaded in carboxymethyl chitosan nanoparticles cross-linked with Ca<sup>2+</sup> ions (CMCS-Ca<sup>2+</sup>). The ultrasonication with experimental design was used to produce CMCS-Ca<sup>2+</sup> nanoparticles loading clindamycin HCl. The model showed that the size of nanoparticles decreased when amplitude and time increased. The nanoparticle size of 318.40 ± 7.56 nm, decreased significantly from 543.63 ± 55.07 nm (<i>p</i> < 0.05), was obtained from 75% of amplitude and 180 s of time, which was one of the optimal conditions. The clindamycin loading content in this condition was 34.68 ± 2.54%. The drug content in nanoparticles showed an inverse relationship with the size of the nanoparticles. The sodium carboxymethylcellulose film loading clindamycin HCl nanoparticles exhibited extended release with 69.88 ± 2.03% drug release at 60 min and a gradual increase to 94.99 ± 4.70% at 24 h, and demonstrated good antibacterial activity against <i>S. aureus</i> and <i>C. acne</i> with 40.72 ± 1.23 and 48.70 ± 1.99 mm of the zone of inhibition at 24 h, respectively. Thus, CMCS-Ca<sup>2+</sup> nanoparticles produced by the ultrasound-assisted technique could be a potential delivery system to modify the drug release of small hydrophilic antibiotics. |
| first_indexed | 2024-03-10T03:47:10Z |
| format | Article |
| id | doaj.art-b07550fcdb8141aaae6488a7a229898d |
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| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:47:10Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b07550fcdb8141aaae6488a7a229898d2023-11-23T09:05:30ZengMDPI AGPolymers2073-43602022-04-01149173610.3390/polym14091736Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl CarrierTanpong Chaiwarit0Sarana Rose Sommano1Pornchai Rachtanapun2Nutthapong Kantrong3Warintorn Ruksiriwanich4Mont Kumpugdee-Vollrath5Pensak Jantrawut6Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandPlant Bioactive Compound Laboratory (BAC), Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandCluster of Research and Development of Pharmaceutical and Natural Products Innovation for Human or Animal, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Restorative Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandLaboratory of Pharmaceutical Technology, Faculty II, Berliner Hochschule für Technik (BHT), 13353 Berlin, GermanyDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandPolymeric nanoparticles are one method to modify the drug release of small hydrophilic molecules. In this study, clindamycin HCl was used as a model drug loaded in carboxymethyl chitosan nanoparticles cross-linked with Ca<sup>2+</sup> ions (CMCS-Ca<sup>2+</sup>). The ultrasonication with experimental design was used to produce CMCS-Ca<sup>2+</sup> nanoparticles loading clindamycin HCl. The model showed that the size of nanoparticles decreased when amplitude and time increased. The nanoparticle size of 318.40 ± 7.56 nm, decreased significantly from 543.63 ± 55.07 nm (<i>p</i> < 0.05), was obtained from 75% of amplitude and 180 s of time, which was one of the optimal conditions. The clindamycin loading content in this condition was 34.68 ± 2.54%. The drug content in nanoparticles showed an inverse relationship with the size of the nanoparticles. The sodium carboxymethylcellulose film loading clindamycin HCl nanoparticles exhibited extended release with 69.88 ± 2.03% drug release at 60 min and a gradual increase to 94.99 ± 4.70% at 24 h, and demonstrated good antibacterial activity against <i>S. aureus</i> and <i>C. acne</i> with 40.72 ± 1.23 and 48.70 ± 1.99 mm of the zone of inhibition at 24 h, respectively. Thus, CMCS-Ca<sup>2+</sup> nanoparticles produced by the ultrasound-assisted technique could be a potential delivery system to modify the drug release of small hydrophilic antibiotics.https://www.mdpi.com/2073-4360/14/9/1736carboxymethyl chitosanexperimental designnanoparticleultrasoundclindamycin |
| spellingShingle | Tanpong Chaiwarit Sarana Rose Sommano Pornchai Rachtanapun Nutthapong Kantrong Warintorn Ruksiriwanich Mont Kumpugdee-Vollrath Pensak Jantrawut Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier Polymers carboxymethyl chitosan experimental design nanoparticle ultrasound clindamycin |
| title | Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier |
| title_full | Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier |
| title_fullStr | Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier |
| title_full_unstemmed | Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier |
| title_short | Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier |
| title_sort | development of carboxymethyl chitosan nanoparticles prepared by ultrasound assisted technique for a clindamycin hcl carrier |
| topic | carboxymethyl chitosan experimental design nanoparticle ultrasound clindamycin |
| url | https://www.mdpi.com/2073-4360/14/9/1736 |
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