In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L.
Caper (Capparis spinosa L.) is a medical plant grown in Jordan. Mass harvesting of caper plants from their origin environments caused a reduction of these germplasm. Therefore, an easy and consistent method for clonal proliferation and callus induction was established for this species. C. spinosa L...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AcademicPres
2024-03-01
|
| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| Subjects: | |
| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13609 |
| _version_ | 1797231355870838784 |
|---|---|
| author | Wesam G. SHAHROUR Mohamad A. SHATNAWI Mohammad AL-ALAWI Rida A. SHIBLI Tamara S. ALQUDAH Majdi M. MAJDALAWI Abdel Rahman AL-TAWAHA Ahmad ALJAMMAL |
| author_facet | Wesam G. SHAHROUR Mohamad A. SHATNAWI Mohammad AL-ALAWI Rida A. SHIBLI Tamara S. ALQUDAH Majdi M. MAJDALAWI Abdel Rahman AL-TAWAHA Ahmad ALJAMMAL |
| author_sort | Wesam G. SHAHROUR |
| collection | DOAJ |
| description |
Caper (Capparis spinosa L.) is a medical plant grown in Jordan. Mass harvesting of caper plants from their origin environments caused a reduction of these germplasm. Therefore, an easy and consistent method for clonal proliferation and callus induction was established for this species. C. spinosa L. in vitro culture affected in MS medium provided by 0.5 mg/L BAP gave 5.9 microshoots/explant. Two months later MS medium supplemented with 2.0 mg/L NAA developed a maximum callus induction of 33.1 mm. Ex vitro, in vitro, and callus growth of C. spinosa L. using ethanolic and methanolic extracts were tested for their antimicrobial activity against different species of bacteria and fungi. Both ex-vitro and in vitro plants exhibited similar antimicrobial activity. Maximum ex vitro plant antibacterial activity was (23 mm ± 0.58 inhibition zone) against Staphylococcus epidermidi. In comparison, callus extracts gave the highest antibacterial activity against Bacillus cereus and Escherichia coli. Moreover, caper plant extracts showed different antifungal effects against the tested fungi species. Investigation of the data showed that ex-vitro extract exhibited maximum antifungal activity compared to in vitro plants. Additionally, exposed Bemisia tabaci 4th nymphal instar to C. spinosa L. extracts suffered mortality ranging from 2 to 28%. In most instances, both ethanolic and methanolic extracts affected the survival of B. tabaci more than the control. The current study confirmed that C. spinosa L. has a wide range of antibacterial, antifungal, and insecticidal activity.
|
| first_indexed | 2024-04-24T15:43:04Z |
| format | Article |
| id | doaj.art-b0765e24442b4842aa615ff4ed7a6b64 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-24T15:43:04Z |
| publishDate | 2024-03-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-b0765e24442b4842aa615ff4ed7a6b642024-04-01T18:46:32ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092024-03-0152110.15835/nbha52113609In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L.Wesam G. SHAHROUR0Mohamad A. SHATNAWI1Mohammad AL-ALAWI2Rida A. SHIBLI3Tamara S. ALQUDAH4Majdi M. MAJDALAWI5Abdel Rahman AL-TAWAHA6Ahmad ALJAMMAL7Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Plant Production and Protection, Al-Salt 19117University of Jordan, Faculty of Agriculture, Department of Horticulture and Crop Sciences, Amman; The University of Jordan, Hamdi Mango Center for Scientific Research (HMCSR), AmmanThe University of Jordan, Hamdi Mango Center for Scientific Research (HMCSR), AmmanAl-Balqa Applied University, Zarqa University College, ZarqaAl Hussein bin Talal University, Department of Biological Sciences, P.O. Box 20, MaanAl-Balqa Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117 Caper (Capparis spinosa L.) is a medical plant grown in Jordan. Mass harvesting of caper plants from their origin environments caused a reduction of these germplasm. Therefore, an easy and consistent method for clonal proliferation and callus induction was established for this species. C. spinosa L. in vitro culture affected in MS medium provided by 0.5 mg/L BAP gave 5.9 microshoots/explant. Two months later MS medium supplemented with 2.0 mg/L NAA developed a maximum callus induction of 33.1 mm. Ex vitro, in vitro, and callus growth of C. spinosa L. using ethanolic and methanolic extracts were tested for their antimicrobial activity against different species of bacteria and fungi. Both ex-vitro and in vitro plants exhibited similar antimicrobial activity. Maximum ex vitro plant antibacterial activity was (23 mm ± 0.58 inhibition zone) against Staphylococcus epidermidi. In comparison, callus extracts gave the highest antibacterial activity against Bacillus cereus and Escherichia coli. Moreover, caper plant extracts showed different antifungal effects against the tested fungi species. Investigation of the data showed that ex-vitro extract exhibited maximum antifungal activity compared to in vitro plants. Additionally, exposed Bemisia tabaci 4th nymphal instar to C. spinosa L. extracts suffered mortality ranging from 2 to 28%. In most instances, both ethanolic and methanolic extracts affected the survival of B. tabaci more than the control. The current study confirmed that C. spinosa L. has a wide range of antibacterial, antifungal, and insecticidal activity. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13609antimicrobial activityBemisia tabaciCapparis spinosa L.in vitro multiplicationinsecticidal activitymedical plant |
| spellingShingle | Wesam G. SHAHROUR Mohamad A. SHATNAWI Mohammad AL-ALAWI Rida A. SHIBLI Tamara S. ALQUDAH Majdi M. MAJDALAWI Abdel Rahman AL-TAWAHA Ahmad ALJAMMAL In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca antimicrobial activity Bemisia tabaci Capparis spinosa L. in vitro multiplication insecticidal activity medical plant |
| title | In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. |
| title_full | In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. |
| title_fullStr | In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. |
| title_full_unstemmed | In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. |
| title_short | In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. |
| title_sort | in vitro multiplication antimicrobial and insecticidal activity of capparis spinosa l |
| topic | antimicrobial activity Bemisia tabaci Capparis spinosa L. in vitro multiplication insecticidal activity medical plant |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13609 |
| work_keys_str_mv | AT wesamgshahrour invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT mohamadashatnawi invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT mohammadalalawi invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT ridaashibli invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT tamarasalqudah invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT majdimmajdalawi invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT abdelrahmanaltawaha invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal AT ahmadaljammal invitromultiplicationantimicrobialandinsecticidalactivityofcapparisspinosal |