Chromogenic Escherichia coli reporter strain for screening DNA damaging agents
Abstract The presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. Her...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-01-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-021-01342-1 |
| _version_ | 1819229342058676224 |
|---|---|
| author | Josué Daniel Mora-Garduño Jessica Tamayo-Nuñez Felipe Padilla-Vaca Fátima Berenice Ramírez-Montiel Ángeles Rangel-Serrano Fernando Santos-Escobar Félix Gutiérrez-Corona Itzel Páramo-Pérez Fernando Anaya-Velázquez Rodolfo García-Contreras Naurú Idalia Vargas-Maya Bernardo Franco |
| author_facet | Josué Daniel Mora-Garduño Jessica Tamayo-Nuñez Felipe Padilla-Vaca Fátima Berenice Ramírez-Montiel Ángeles Rangel-Serrano Fernando Santos-Escobar Félix Gutiérrez-Corona Itzel Páramo-Pérez Fernando Anaya-Velázquez Rodolfo García-Contreras Naurú Idalia Vargas-Maya Bernardo Franco |
| author_sort | Josué Daniel Mora-Garduño |
| collection | DOAJ |
| description | Abstract The presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. Here, an Escherichia coli reporter strain expressing a chromoprotein capable of interacting with soil or water samples and responding to DNA damaging compounds is validated. The reporter strain generates a qualitative signal and is based on the expression of the coral chromoprotein AmilCP under the control of the recA promoter. This strain can be used simply by applying soil or water samples directly and rendering activation upon DNA damage. This reporter strain responds to agents that damage DNA (with an apparent detection limit of 1 µg of mitomycin C) without observable response to membrane integrity damage, protein folding or oxidative stress generating agents, in the latter case, DNA damage was observed. The developed reporter strain reported here is effective for the detection of DNA damaging agents present in soils samples. In a proof-of-concept analysis using soil containing chromium, showing activation at 15.56 mg/L of Cr(VI) present in soil and leached samples and is consistent with Cr(III) toxicity at high concentrations (130 µg). Our findings suggest that chromogenic reporter strains can be applied for simple screening, thus reducing the number of samples requiring analytical techniques. |
| first_indexed | 2024-12-23T11:11:39Z |
| format | Article |
| id | doaj.art-b0774a046d4447838399055c95b41af9 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-23T11:11:39Z |
| publishDate | 2022-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-b0774a046d4447838399055c95b41af92022-12-21T17:49:20ZengSpringerOpenAMB Express2191-08552022-01-0112111110.1186/s13568-021-01342-1Chromogenic Escherichia coli reporter strain for screening DNA damaging agentsJosué Daniel Mora-Garduño0Jessica Tamayo-Nuñez1Felipe Padilla-Vaca2Fátima Berenice Ramírez-Montiel3Ángeles Rangel-Serrano4Fernando Santos-Escobar5Félix Gutiérrez-Corona6Itzel Páramo-Pérez7Fernando Anaya-Velázquez8Rodolfo García-Contreras9Naurú Idalia Vargas-Maya10Bernardo Franco11Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Microbiología y Parasitología, Facultad de Medicina, UNAMDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de GuanajuatoAbstract The presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. Here, an Escherichia coli reporter strain expressing a chromoprotein capable of interacting with soil or water samples and responding to DNA damaging compounds is validated. The reporter strain generates a qualitative signal and is based on the expression of the coral chromoprotein AmilCP under the control of the recA promoter. This strain can be used simply by applying soil or water samples directly and rendering activation upon DNA damage. This reporter strain responds to agents that damage DNA (with an apparent detection limit of 1 µg of mitomycin C) without observable response to membrane integrity damage, protein folding or oxidative stress generating agents, in the latter case, DNA damage was observed. The developed reporter strain reported here is effective for the detection of DNA damaging agents present in soils samples. In a proof-of-concept analysis using soil containing chromium, showing activation at 15.56 mg/L of Cr(VI) present in soil and leached samples and is consistent with Cr(III) toxicity at high concentrations (130 µg). Our findings suggest that chromogenic reporter strains can be applied for simple screening, thus reducing the number of samples requiring analytical techniques.https://doi.org/10.1186/s13568-021-01342-1DNA damageReporter strainChromogenic responseAmilCPEnvironmental samplesChromium pollution |
| spellingShingle | Josué Daniel Mora-Garduño Jessica Tamayo-Nuñez Felipe Padilla-Vaca Fátima Berenice Ramírez-Montiel Ángeles Rangel-Serrano Fernando Santos-Escobar Félix Gutiérrez-Corona Itzel Páramo-Pérez Fernando Anaya-Velázquez Rodolfo García-Contreras Naurú Idalia Vargas-Maya Bernardo Franco Chromogenic Escherichia coli reporter strain for screening DNA damaging agents AMB Express DNA damage Reporter strain Chromogenic response AmilCP Environmental samples Chromium pollution |
| title | Chromogenic Escherichia coli reporter strain for screening DNA damaging agents |
| title_full | Chromogenic Escherichia coli reporter strain for screening DNA damaging agents |
| title_fullStr | Chromogenic Escherichia coli reporter strain for screening DNA damaging agents |
| title_full_unstemmed | Chromogenic Escherichia coli reporter strain for screening DNA damaging agents |
| title_short | Chromogenic Escherichia coli reporter strain for screening DNA damaging agents |
| title_sort | chromogenic escherichia coli reporter strain for screening dna damaging agents |
| topic | DNA damage Reporter strain Chromogenic response AmilCP Environmental samples Chromium pollution |
| url | https://doi.org/10.1186/s13568-021-01342-1 |
| work_keys_str_mv | AT josuedanielmoragarduno chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT jessicatamayonunez chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT felipepadillavaca chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT fatimabereniceramirezmontiel chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT angelesrangelserrano chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT fernandosantosescobar chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT felixgutierrezcorona chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT itzelparamoperez chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT fernandoanayavelazquez chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT rodolfogarciacontreras chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT nauruidaliavargasmaya chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents AT bernardofranco chromogenicescherichiacolireporterstrainforscreeningdnadamagingagents |