Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk
Quantitative detection of veterinary drug residues in animal-derived food is of great significance. In this work, a simple and label-free electrochemical aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed based on a new biosensing method, where...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/16/6074 |
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| author | Fang Li Shuyue Xiong Pei Zhao Panpan Dong Zijian Wu |
| author_facet | Fang Li Shuyue Xiong Pei Zhao Panpan Dong Zijian Wu |
| author_sort | Fang Li |
| collection | DOAJ |
| description | Quantitative detection of veterinary drug residues in animal-derived food is of great significance. In this work, a simple and label-free electrochemical aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed based on a new biosensing method, where the single- or few-layer Ti<sub>3</sub>C<sub>2</sub> MXene nanosheets functionalized via the specific aptamer by self-assembly were used as electrode modifiers for a glassy carbon electrode (aptamer/Ti<sub>3</sub>C<sub>2</sub> MXene/GCE). Differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscope (AFM), and so on were utilized for electrochemical and morphological characterization. Under the optimized conditions, the constructed aptasensor exhibited excellent performance with a wider linearity to CAP in the range from 10 fM to 1 μM and a low detection limit of 1 fM. Aptamer/Ti<sub>3</sub>C<sub>2</sub> MXene/GCE demonstrated remarkable selectivity over other potentially interfering antibiotics, as well as exceptional reproducibility and stability. In addition, the aptasensor was successfully applied to determine CAP in milk with acceptable recovery values of 96.13% to 108.15% and relative standard deviations below 9%. Therefore, the proposed electrochemical aptasensor is an excellent alternative for determining CAP in food samples. |
| first_indexed | 2024-03-10T23:41:44Z |
| format | Article |
| id | doaj.art-b0784766a53e4068b63c7976eec3a68e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T23:41:44Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b0784766a53e4068b63c7976eec3a68e2023-11-19T02:24:24ZengMDPI AGMolecules1420-30492023-08-012816607410.3390/molecules28166074Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in MilkFang Li0Shuyue Xiong1Pei Zhao2Panpan Dong3Zijian Wu4Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaTianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaTianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaTianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaTianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaQuantitative detection of veterinary drug residues in animal-derived food is of great significance. In this work, a simple and label-free electrochemical aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed based on a new biosensing method, where the single- or few-layer Ti<sub>3</sub>C<sub>2</sub> MXene nanosheets functionalized via the specific aptamer by self-assembly were used as electrode modifiers for a glassy carbon electrode (aptamer/Ti<sub>3</sub>C<sub>2</sub> MXene/GCE). Differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscope (AFM), and so on were utilized for electrochemical and morphological characterization. Under the optimized conditions, the constructed aptasensor exhibited excellent performance with a wider linearity to CAP in the range from 10 fM to 1 μM and a low detection limit of 1 fM. Aptamer/Ti<sub>3</sub>C<sub>2</sub> MXene/GCE demonstrated remarkable selectivity over other potentially interfering antibiotics, as well as exceptional reproducibility and stability. In addition, the aptasensor was successfully applied to determine CAP in milk with acceptable recovery values of 96.13% to 108.15% and relative standard deviations below 9%. Therefore, the proposed electrochemical aptasensor is an excellent alternative for determining CAP in food samples.https://www.mdpi.com/1420-3049/28/16/6074label-freeelectrochemical aptasensorchloramphenicolTi<sub>3</sub>C<sub>2</sub> MXene nanosheets |
| spellingShingle | Fang Li Shuyue Xiong Pei Zhao Panpan Dong Zijian Wu Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk Molecules label-free electrochemical aptasensor chloramphenicol Ti<sub>3</sub>C<sub>2</sub> MXene nanosheets |
| title | Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk |
| title_full | Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk |
| title_fullStr | Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk |
| title_full_unstemmed | Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk |
| title_short | Few Layer Ti<sub>3</sub>C<sub>2</sub> MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk |
| title_sort | few layer ti sub 3 sub c sub 2 sub mxene based label free aptasensor for ultrasensitive determination of chloramphenicol in milk |
| topic | label-free electrochemical aptasensor chloramphenicol Ti<sub>3</sub>C<sub>2</sub> MXene nanosheets |
| url | https://www.mdpi.com/1420-3049/28/16/6074 |
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