Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice
Gelsemine is an active principle and a major alkaloid found in <i>Gelsemium</i> genus of plants belonging to the <i>Loganiaceae</i> family. The aim of the present study was to explore whether gelsemine exerts anxiolytic effects on a mouse model of chronic-unpredictable-mild-s...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2076-3425/12/2/191 |
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| author | Hui Yu Mo-Huan Tang Zi-Yue Zeng Si-Juan Huang Xiao-Feng Zheng Zhao-Ying Liu |
| author_facet | Hui Yu Mo-Huan Tang Zi-Yue Zeng Si-Juan Huang Xiao-Feng Zheng Zhao-Ying Liu |
| author_sort | Hui Yu |
| collection | DOAJ |
| description | Gelsemine is an active principle and a major alkaloid found in <i>Gelsemium</i> genus of plants belonging to the <i>Loganiaceae</i> family. The aim of the present study was to explore whether gelsemine exerts anxiolytic effects on a mouse model of chronic-unpredictable-mild-stress (CUMS)-induced anxiety-like behaviors. NOD-like receptor protein 3 (NLRP3) inflammasome, downregulated cAMP-response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) were also evaluated as potential mechanisms. First, gelsemine reversed a CUMS-induced decrease in body-weight gain in mice. Next, gelsemine alleviated CUMS-induced anxiety-like behaviors, as evidenced by the increased distance traveled in the central zone of the open-field test, both the increased percentage of time spent and distance traveled in the light compartment, the increased number of transitions between compartments in the light/dark-transition test, and the increased percentage of entries and time spent in the open arm of the elevated plus-maze. In addition, gelsemine decreased the levels of pro-inflammatory cytokines, including interleukin (IL)-1β and IL-6, in the hypothalamus and hippocampus of CUMS mice. Interestingly, further investigations revealed that gelsemine inhibited the CUMS-induced activation of NLRP3-inflammasome pathways and downregulated CREB and BDNF overexpression in the hypothalamus. In summary, gelsemine alleviated anxiety-like behaviors in the CUMS-induced mouse model. Gelsemine exerted its anxiolytic effects by modulating the NLRP3 and CREB/BDNF pathways. |
| first_indexed | 2024-03-09T22:28:12Z |
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| last_indexed | 2024-03-09T22:28:12Z |
| publishDate | 2022-01-01 |
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| series | Brain Sciences |
| spelling | doaj.art-b0797f00fdfd47bc9dac02c34310e8c32023-11-23T19:03:01ZengMDPI AGBrain Sciences2076-34252022-01-0112219110.3390/brainsci12020191Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in MiceHui Yu0Mo-Huan Tang1Zi-Yue Zeng2Si-Juan Huang3Xiao-Feng Zheng4Zhao-Ying Liu5College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaGelsemine is an active principle and a major alkaloid found in <i>Gelsemium</i> genus of plants belonging to the <i>Loganiaceae</i> family. The aim of the present study was to explore whether gelsemine exerts anxiolytic effects on a mouse model of chronic-unpredictable-mild-stress (CUMS)-induced anxiety-like behaviors. NOD-like receptor protein 3 (NLRP3) inflammasome, downregulated cAMP-response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) were also evaluated as potential mechanisms. First, gelsemine reversed a CUMS-induced decrease in body-weight gain in mice. Next, gelsemine alleviated CUMS-induced anxiety-like behaviors, as evidenced by the increased distance traveled in the central zone of the open-field test, both the increased percentage of time spent and distance traveled in the light compartment, the increased number of transitions between compartments in the light/dark-transition test, and the increased percentage of entries and time spent in the open arm of the elevated plus-maze. In addition, gelsemine decreased the levels of pro-inflammatory cytokines, including interleukin (IL)-1β and IL-6, in the hypothalamus and hippocampus of CUMS mice. Interestingly, further investigations revealed that gelsemine inhibited the CUMS-induced activation of NLRP3-inflammasome pathways and downregulated CREB and BDNF overexpression in the hypothalamus. In summary, gelsemine alleviated anxiety-like behaviors in the CUMS-induced mouse model. Gelsemine exerted its anxiolytic effects by modulating the NLRP3 and CREB/BDNF pathways.https://www.mdpi.com/2076-3425/12/2/191gelsemineanxietyBDNFNLRP3 inflammasome<i>Gelsemium</i> |
| spellingShingle | Hui Yu Mo-Huan Tang Zi-Yue Zeng Si-Juan Huang Xiao-Feng Zheng Zhao-Ying Liu Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice Brain Sciences gelsemine anxiety BDNF NLRP3 inflammasome <i>Gelsemium</i> |
| title | Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice |
| title_full | Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice |
| title_fullStr | Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice |
| title_full_unstemmed | Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice |
| title_short | Suppressive Effects of Gelsemine on Anxiety-like Behaviors Induced by Chronic Unpredictable Mild Stress in Mice |
| title_sort | suppressive effects of gelsemine on anxiety like behaviors induced by chronic unpredictable mild stress in mice |
| topic | gelsemine anxiety BDNF NLRP3 inflammasome <i>Gelsemium</i> |
| url | https://www.mdpi.com/2076-3425/12/2/191 |
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