Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction
Abstract Objective Dichelobacter nodosus is the causative agent of footrot in sheep. Ovine footrot is a major problem in Australia that results in large economic losses and a represents a very significant animal welfare issue. D. nodosus is divided into 10 serogroups (A–I, M), based on sequence vari...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3229-5 |
| _version_ | 1819225600263454720 |
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| author | Nickala Best Jacek Gwozdz Robert Suter Grant Rawlin Travis Beddoe |
| author_facet | Nickala Best Jacek Gwozdz Robert Suter Grant Rawlin Travis Beddoe |
| author_sort | Nickala Best |
| collection | DOAJ |
| description | Abstract Objective Dichelobacter nodosus is the causative agent of footrot in sheep. Ovine footrot is a major problem in Australia that results in large economic losses and a represents a very significant animal welfare issue. D. nodosus is divided into 10 serogroups (A–I, M), based on sequence variation in the type IV fimbriae gene, fimA. Control of the bacteria is possible through use of serogroup-specific vaccination, however traditional identification of the serogroups of D. nodosus on infected sheep is time-consuming and costly. With the aim of reducing time and cost, a PCR assay was used to identify serogroups of D. nodosus directly from foot swabs of infected sheep in Victoria. Results It was shown that serogroup B was most common (10 locations), followed by A, G and H (4 locations), I and C (2 locations), D, E and F (1 location). Infections with multiple serotypes were observed in 50% of farms, with the remaining 50% having only a single serogroup detected. The ability to identify serogroups quickly and cheaply direct from foot swabs will aid the understanding of the epidemiology of D. nodosus and support control programs. |
| first_indexed | 2024-12-23T10:12:10Z |
| format | Article |
| id | doaj.art-b07db3d87f5f438b9044bfff97d70e13 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-23T10:12:10Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-b07db3d87f5f438b9044bfff97d70e132022-12-21T17:50:56ZengBMCBMC Research Notes1756-05002018-02-011111610.1186/s13104-018-3229-5Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reactionNickala Best0Jacek Gwozdz1Robert Suter2Grant Rawlin3Travis Beddoe4Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe UniversityDepartment of Economic Development, Jobs, Transport and Resources Centre for AgriBioscience (AgriBio), Victorian GovernmentDepartment of Economic Development, Jobs, Transport and Resources, Victorian GovernmentDepartment of Economic Development, Jobs, Transport and Resources Centre for AgriBioscience (AgriBio), Victorian GovernmentDepartment of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe UniversityAbstract Objective Dichelobacter nodosus is the causative agent of footrot in sheep. Ovine footrot is a major problem in Australia that results in large economic losses and a represents a very significant animal welfare issue. D. nodosus is divided into 10 serogroups (A–I, M), based on sequence variation in the type IV fimbriae gene, fimA. Control of the bacteria is possible through use of serogroup-specific vaccination, however traditional identification of the serogroups of D. nodosus on infected sheep is time-consuming and costly. With the aim of reducing time and cost, a PCR assay was used to identify serogroups of D. nodosus directly from foot swabs of infected sheep in Victoria. Results It was shown that serogroup B was most common (10 locations), followed by A, G and H (4 locations), I and C (2 locations), D, E and F (1 location). Infections with multiple serotypes were observed in 50% of farms, with the remaining 50% having only a single serogroup detected. The ability to identify serogroups quickly and cheaply direct from foot swabs will aid the understanding of the epidemiology of D. nodosus and support control programs.http://link.springer.com/article/10.1186/s13104-018-3229-5FootrotSerogroupPCR |
| spellingShingle | Nickala Best Jacek Gwozdz Robert Suter Grant Rawlin Travis Beddoe Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction BMC Research Notes Footrot Serogroup PCR |
| title | Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction |
| title_full | Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction |
| title_fullStr | Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction |
| title_full_unstemmed | Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction |
| title_short | Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction |
| title_sort | direct serogrouping of dichelobacter nodosus from victorian farms using conventional multiplex polymerase chain reaction |
| topic | Footrot Serogroup PCR |
| url | http://link.springer.com/article/10.1186/s13104-018-3229-5 |
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