The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle
Electron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral inf...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.02014/full |
| _version_ | 1819160393464938496 |
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| author | Djamal Brahim Belhaouari Anthony Fontanini Jean-Pierre Baudoin Gabriel Haddad Marion Le Bideau Jacques Yaacoub Bou Khalil Didier Raoult Bernard La Scola Bernard La Scola |
| author_facet | Djamal Brahim Belhaouari Anthony Fontanini Jean-Pierre Baudoin Gabriel Haddad Marion Le Bideau Jacques Yaacoub Bou Khalil Didier Raoult Bernard La Scola Bernard La Scola |
| author_sort | Djamal Brahim Belhaouari |
| collection | DOAJ |
| description | Electron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral infections. In the present study, we used scanning electron microscopy (SEM) to study the infectious cycle of SARS-CoV-2 in Vero E6 cells and we controlled some key findings by classical transmission electronic microscopy (TEM). The replication cycle of the virus was followed from 1 to 36 h post-infection. Our results revealed that SARS-CoV-2 infected the cells through membrane fusion. Particles are formed in the peri-nuclear region from a budding of the endoplasmic reticulum-Golgi apparatus complex into morphogenesis matrix vesicae. New SARS-CoV-2 particles were expelled from the cells, through cell lysis or by fusion of virus containing vacuoles with the cell plasma membrane. Overall, this cycle is highly comparable to that of SARS-CoV. By providing a detailed and complete SARS-CoV-2 infectious cycle, SEM proves to be a very rapid and efficient tool compared to classical TEM. |
| first_indexed | 2024-12-22T16:55:44Z |
| format | Article |
| id | doaj.art-b07ea599079942398c8a2d697c8f2fda |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T16:55:44Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b07ea599079942398c8a2d697c8f2fda2022-12-21T18:19:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-08-011110.3389/fmicb.2020.02014562013The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious CycleDjamal Brahim Belhaouari0Anthony Fontanini1Jean-Pierre Baudoin2Gabriel Haddad3Marion Le Bideau4Jacques Yaacoub Bou Khalil5Didier Raoult6Bernard La Scola7Bernard La Scola8Microbes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique – Hôpitaux de Marseille (AP-HM), Aix-Marseille University, Marseille, FranceIHU Méditerranée Infection, Marseille, FranceMicrobes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique – Hôpitaux de Marseille (AP-HM), Aix-Marseille University, Marseille, FranceMicrobes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique – Hôpitaux de Marseille (AP-HM), Aix-Marseille University, Marseille, FranceMicrobes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique – Hôpitaux de Marseille (AP-HM), Aix-Marseille University, Marseille, FranceIHU Méditerranée Infection, Marseille, FranceMicrobes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique – Hôpitaux de Marseille (AP-HM), Aix-Marseille University, Marseille, FranceMicrobes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique – Hôpitaux de Marseille (AP-HM), Aix-Marseille University, Marseille, FranceIHU Méditerranée Infection, Marseille, FranceElectron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral infections. In the present study, we used scanning electron microscopy (SEM) to study the infectious cycle of SARS-CoV-2 in Vero E6 cells and we controlled some key findings by classical transmission electronic microscopy (TEM). The replication cycle of the virus was followed from 1 to 36 h post-infection. Our results revealed that SARS-CoV-2 infected the cells through membrane fusion. Particles are formed in the peri-nuclear region from a budding of the endoplasmic reticulum-Golgi apparatus complex into morphogenesis matrix vesicae. New SARS-CoV-2 particles were expelled from the cells, through cell lysis or by fusion of virus containing vacuoles with the cell plasma membrane. Overall, this cycle is highly comparable to that of SARS-CoV. By providing a detailed and complete SARS-CoV-2 infectious cycle, SEM proves to be a very rapid and efficient tool compared to classical TEM.https://www.frontiersin.org/article/10.3389/fmicb.2020.02014/fullSARS-CoV-2infectious cycleVero E6 cellsscanning electron microscopyCoronavirus |
| spellingShingle | Djamal Brahim Belhaouari Anthony Fontanini Jean-Pierre Baudoin Gabriel Haddad Marion Le Bideau Jacques Yaacoub Bou Khalil Didier Raoult Bernard La Scola Bernard La Scola The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle Frontiers in Microbiology SARS-CoV-2 infectious cycle Vero E6 cells scanning electron microscopy Coronavirus |
| title | The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle |
| title_full | The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle |
| title_fullStr | The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle |
| title_full_unstemmed | The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle |
| title_short | The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle |
| title_sort | strengths of scanning electron microscopy in deciphering sars cov 2 infectious cycle |
| topic | SARS-CoV-2 infectious cycle Vero E6 cells scanning electron microscopy Coronavirus |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.02014/full |
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