Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae)
By relating genetic divergence at neutral loci, phenotypic variation, and geographic and environmental distances, it is possible to dissect micro-evolutionary scenarios involving natural selection and neutral evolution. In this work, we tested the patterns of intraspecific genetic and phenotypic var...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/12/10/1355 |
| _version_ | 1797574679768072192 |
|---|---|
| author | Jacopo Franzoni Giovanni Astuti Lorenzo Peruzzi |
| author_facet | Jacopo Franzoni Giovanni Astuti Lorenzo Peruzzi |
| author_sort | Jacopo Franzoni |
| collection | DOAJ |
| description | By relating genetic divergence at neutral loci, phenotypic variation, and geographic and environmental distances, it is possible to dissect micro-evolutionary scenarios involving natural selection and neutral evolution. In this work, we tested the patterns of intraspecific genetic and phenotypic variation along an elevational gradient, using <i>Dianthus virgineus</i> as study system. We genotyped genome-wide SNPs through ddRAD sequencing and quantified phenotypic variation through multivariate morphological variation. We assessed patterns of variation by testing the statistical association between genetic, phenotypic, geographic, and elevational distances and explored the role of genetic drift and selection by comparing the Fst and Pst of morphometric traits. We revealed a weak genetic structure related to geographic distance among populations, but we excluded the predominant role of genetic drift acting on phenotypic traits. A high degree of phenotypic differentiation with respect to genetic divergence at neutral loci allowed us to hypothesize the effect of selection, putatively fuelled by changing conditions at different sites, on morphological traits. Thus, natural selection acting despite low genetic divergence at neutral loci can be hypothesized as a putative driver explaining the observed patterns of variation. |
| first_indexed | 2024-03-10T21:25:45Z |
| format | Article |
| id | doaj.art-b07f702a5e9042ce95a7512c855f4ce9 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T21:25:45Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-b07f702a5e9042ce95a7512c855f4ce92023-11-19T15:44:09ZengMDPI AGBiology2079-77372023-10-011210135510.3390/biology12101355Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae)Jacopo Franzoni0Giovanni Astuti1Lorenzo Peruzzi2PLANTSEED Lab, Department of Biology, University of Pisa, 56127 Pisa, ItalyBotanic Garden and Museum, University of Pisa, 56126 Pisa, ItalyPLANTSEED Lab, Department of Biology, University of Pisa, 56127 Pisa, ItalyBy relating genetic divergence at neutral loci, phenotypic variation, and geographic and environmental distances, it is possible to dissect micro-evolutionary scenarios involving natural selection and neutral evolution. In this work, we tested the patterns of intraspecific genetic and phenotypic variation along an elevational gradient, using <i>Dianthus virgineus</i> as study system. We genotyped genome-wide SNPs through ddRAD sequencing and quantified phenotypic variation through multivariate morphological variation. We assessed patterns of variation by testing the statistical association between genetic, phenotypic, geographic, and elevational distances and explored the role of genetic drift and selection by comparing the Fst and Pst of morphometric traits. We revealed a weak genetic structure related to geographic distance among populations, but we excluded the predominant role of genetic drift acting on phenotypic traits. A high degree of phenotypic differentiation with respect to genetic divergence at neutral loci allowed us to hypothesize the effect of selection, putatively fuelled by changing conditions at different sites, on morphological traits. Thus, natural selection acting despite low genetic divergence at neutral loci can be hypothesized as a putative driver explaining the observed patterns of variation.https://www.mdpi.com/2079-7737/12/10/1355elevationevolutionFstMediterraneanmorphometryIBD |
| spellingShingle | Jacopo Franzoni Giovanni Astuti Lorenzo Peruzzi Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae) Biology elevation evolution Fst Mediterranean morphometry IBD |
| title | Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae) |
| title_full | Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae) |
| title_fullStr | Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae) |
| title_full_unstemmed | Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae) |
| title_short | Weak Genetic Isolation and Putative Phenotypic Selection in the Wild Carnation <i>Dianthus virgineus</i> (Caryophyllaceae) |
| title_sort | weak genetic isolation and putative phenotypic selection in the wild carnation i dianthus virgineus i caryophyllaceae |
| topic | elevation evolution Fst Mediterranean morphometry IBD |
| url | https://www.mdpi.com/2079-7737/12/10/1355 |
| work_keys_str_mv | AT jacopofranzoni weakgeneticisolationandputativephenotypicselectioninthewildcarnationidianthusvirgineusicaryophyllaceae AT giovanniastuti weakgeneticisolationandputativephenotypicselectioninthewildcarnationidianthusvirgineusicaryophyllaceae AT lorenzoperuzzi weakgeneticisolationandputativephenotypicselectioninthewildcarnationidianthusvirgineusicaryophyllaceae |