A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>
The conidia produced by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc), the causative agent of Fusarium Wilt of Banana (FWB), play central roles in the disease cycle, as the pathogen lacks a sexual reproduction process. Until now, the molecular regulation network of as...
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MDPI AG
2023-12-01
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| Series: | Journal of Fungi |
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| author | Songmao Lu Huobing Deng Yaqi Lin Meimei Huang Haixia You Yan Zhang Weijian Zhuang Guodong Lu Yingzi Yun |
| author_facet | Songmao Lu Huobing Deng Yaqi Lin Meimei Huang Haixia You Yan Zhang Weijian Zhuang Guodong Lu Yingzi Yun |
| author_sort | Songmao Lu |
| collection | DOAJ |
| description | The conidia produced by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc), the causative agent of Fusarium Wilt of Banana (FWB), play central roles in the disease cycle, as the pathogen lacks a sexual reproduction process. Until now, the molecular regulation network of asexual sporogenesis has not been clearly understood in Foc. Herein, we identified and functionally characterized thirteen (13) putative sporulation-responsive genes in Foc, namely <i>FocmedA(a)</i>, <i>FocmedA(b)</i>, <i>abaA-L</i>, <i>FocflbA</i>, <i>FocflbB</i>, <i>FocflbC</i>, <i>FocflbD</i>, <i>FocstuA</i>, <i>FocveA</i>, <i>FocvelB</i>, <i>wetA-L</i>, <i>FocfluG</i> and <i>Foclae1</i>. We demonstrated that <i>FocmedA(a)</i>, <i>abaA-L</i>, <i>wetA-L</i>, <i>FocflbA</i>, <i>FocflbD</i>, <i>FocstuA</i>, <i>FocveA</i> and <i>Foclae1</i> mediate conidiophore formation, whereas <i>FocmedA(a)</i> and <i>abaA-L</i> are important for phialide formation and conidiophore formation. The expression level of <i>abaA-L</i> was significantly decreased in the Δ<i>FocmedA(a)</i> mutant, and yeast one-hybrid and ChIP-qPCR analyses further confirmed that FocMedA(a) could bind to the promoter of <i>abaA-L</i> during micro- and macroconidiation. Moreover, the transcript abundance of the <i>wetA-L</i> gene was significantly reduced in the Δ<i>abaA-L</i> mutant, and it not only was found to function as an activator of micro- and macroconidium formation but also served as a repressor of chlamydospore production. In addition, the deletions of <i>FocflbB</i>, <i>FocflbC</i>, <i>FocstuA</i> and <i>Foclae1</i> resulted in increased chlamydosporulation, whereas <i>FocflbD</i> and <i>FocvelB</i> gene deletions reduced chlamydosporulation. Furthermore, <i>FocflbC</i>, <i>FocflbD</i>, <i>Foclae1</i> and <i>FocmedA(a)</i> were found to be important regulators for pathogenicity and fusaric acid synthesis in Foc. The present study therefore advances our understanding of the regulation pathways of the asexual development and functional interdependence of sporulation-responsive genes in Foc. |
| first_indexed | 2024-03-08T10:45:34Z |
| format | Article |
| id | doaj.art-b083000371ce475c99dea1a189d6ae59 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-08T10:45:34Z |
| publishDate | 2023-12-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-b083000371ce475c99dea1a189d6ae592024-01-26T17:18:02ZengMDPI AGJournal of Fungi2309-608X2023-12-01101110.3390/jof10010001A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>Songmao Lu0Huobing Deng1Yaqi Lin2Meimei Huang3Haixia You4Yan Zhang5Weijian Zhuang6Guodong Lu7Yingzi Yun8State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350001, ChinaThe conidia produced by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc), the causative agent of Fusarium Wilt of Banana (FWB), play central roles in the disease cycle, as the pathogen lacks a sexual reproduction process. Until now, the molecular regulation network of asexual sporogenesis has not been clearly understood in Foc. Herein, we identified and functionally characterized thirteen (13) putative sporulation-responsive genes in Foc, namely <i>FocmedA(a)</i>, <i>FocmedA(b)</i>, <i>abaA-L</i>, <i>FocflbA</i>, <i>FocflbB</i>, <i>FocflbC</i>, <i>FocflbD</i>, <i>FocstuA</i>, <i>FocveA</i>, <i>FocvelB</i>, <i>wetA-L</i>, <i>FocfluG</i> and <i>Foclae1</i>. We demonstrated that <i>FocmedA(a)</i>, <i>abaA-L</i>, <i>wetA-L</i>, <i>FocflbA</i>, <i>FocflbD</i>, <i>FocstuA</i>, <i>FocveA</i> and <i>Foclae1</i> mediate conidiophore formation, whereas <i>FocmedA(a)</i> and <i>abaA-L</i> are important for phialide formation and conidiophore formation. The expression level of <i>abaA-L</i> was significantly decreased in the Δ<i>FocmedA(a)</i> mutant, and yeast one-hybrid and ChIP-qPCR analyses further confirmed that FocMedA(a) could bind to the promoter of <i>abaA-L</i> during micro- and macroconidiation. Moreover, the transcript abundance of the <i>wetA-L</i> gene was significantly reduced in the Δ<i>abaA-L</i> mutant, and it not only was found to function as an activator of micro- and macroconidium formation but also served as a repressor of chlamydospore production. In addition, the deletions of <i>FocflbB</i>, <i>FocflbC</i>, <i>FocstuA</i> and <i>Foclae1</i> resulted in increased chlamydosporulation, whereas <i>FocflbD</i> and <i>FocvelB</i> gene deletions reduced chlamydosporulation. Furthermore, <i>FocflbC</i>, <i>FocflbD</i>, <i>Foclae1</i> and <i>FocmedA(a)</i> were found to be important regulators for pathogenicity and fusaric acid synthesis in Foc. The present study therefore advances our understanding of the regulation pathways of the asexual development and functional interdependence of sporulation-responsive genes in Foc.https://www.mdpi.com/2309-608X/10/1/1asexual reproduction<i>Fusarium oxysporum</i> f. sp. <i>cubense</i>genessporogenesis regulation |
| spellingShingle | Songmao Lu Huobing Deng Yaqi Lin Meimei Huang Haixia You Yan Zhang Weijian Zhuang Guodong Lu Yingzi Yun A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Journal of Fungi asexual reproduction <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> genes sporogenesis regulation |
| title | A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_full | A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_fullStr | A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_full_unstemmed | A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_short | A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_sort | network of sporogenesis responsive genes regulates the growth asexual sporogenesis pathogenesis and fusaric acid production of i fusarium oxysporum i f sp i cubense i |
| topic | asexual reproduction <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> genes sporogenesis regulation |
| url | https://www.mdpi.com/2309-608X/10/1/1 |
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