Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia
The level of the products of lipid peroxidation measured as the thiobarbituric acid reactive substances (TBARS) and activity of enzymes of glutathione antioxidant protection system in spermatozoa were studied. It was shown that mean levels of TBARS in sperm cells of infertile men with oligozoo-, ast...
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Львівський національний університет імені Івана Франка
2017-03-01
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| Series: | Біологічні студії |
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| Online Access: | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/8 |
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| author | R. V. Fafula О. K. Оnufrovych D. Z. Vorobets U. P. Iefremova Z. D. Vorobets |
| author_facet | R. V. Fafula О. K. Оnufrovych D. Z. Vorobets U. P. Iefremova Z. D. Vorobets |
| author_sort | R. V. Fafula |
| collection | DOAJ |
| description | The level of the products of lipid peroxidation measured as the thiobarbituric acid reactive substances (TBARS) and activity of enzymes of glutathione antioxidant protection system in spermatozoa were studied. It was shown that mean levels of TBARS in sperm cells of infertile men with oligozoo-, astenozoo-, oligoastenozoo- and leucocytospermia were 1.6-, 2.0-, 2.0- and 2.7-fold higher, respectively, compared with such levels in the fertile men. The glutathione peroxidase activity was decreased 2.2-fold in patients with oligozoospermia, 2.8-fold in patients with astenozoo- and oligoastenozoospermia and 3.8-fold in patients with leucocytospermia. It was found that glutathione reductase activity in patients with oligozoo-, astenozoo-, oligoastenozoo- and leucocytospermia were 2.3-, 1.6-, 1.7- and 3.3-fold lower than that in normozoospermic men, respectively. In addition, the glutathione transferase activity was decreased 1.8–2.1-fold in patients with oligozoo-, astenozoo- and oligoastenozoospermia and 5.0-fold in patients with leucocytospermia in comparison with healthy donors. The most significant changes were observed in the infertile men with leucocytospermia. This manifestation could be explained by the fact that white blood cells stimulate the formation of reactive oxygen species, induction and development of oxidative stress in sperm cells. |
| first_indexed | 2024-12-13T22:18:32Z |
| format | Article |
| id | doaj.art-b083f7f72555497eb34035ac94a70a44 |
| institution | Directory Open Access Journal |
| issn | 1996-4536 2311-0783 |
| language | English |
| last_indexed | 2024-12-13T22:18:32Z |
| publishDate | 2017-03-01 |
| publisher | Львівський національний університет імені Івана Франка |
| record_format | Article |
| series | Біологічні студії |
| spelling | doaj.art-b083f7f72555497eb34035ac94a70a442022-12-21T23:29:27ZengЛьвівський національний університет імені Івана ФранкаБіологічні студії1996-45362311-07832017-03-01111172410.30970/sbi.1101.512Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermiaR. V. Fafula0О. K. Оnufrovych1D. Z. Vorobets2U. P. Iefremova3Z. D. Vorobets4Danylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineThe level of the products of lipid peroxidation measured as the thiobarbituric acid reactive substances (TBARS) and activity of enzymes of glutathione antioxidant protection system in spermatozoa were studied. It was shown that mean levels of TBARS in sperm cells of infertile men with oligozoo-, astenozoo-, oligoastenozoo- and leucocytospermia were 1.6-, 2.0-, 2.0- and 2.7-fold higher, respectively, compared with such levels in the fertile men. The glutathione peroxidase activity was decreased 2.2-fold in patients with oligozoospermia, 2.8-fold in patients with astenozoo- and oligoastenozoospermia and 3.8-fold in patients with leucocytospermia. It was found that glutathione reductase activity in patients with oligozoo-, astenozoo-, oligoastenozoo- and leucocytospermia were 2.3-, 1.6-, 1.7- and 3.3-fold lower than that in normozoospermic men, respectively. In addition, the glutathione transferase activity was decreased 1.8–2.1-fold in patients with oligozoo-, astenozoo- and oligoastenozoospermia and 5.0-fold in patients with leucocytospermia in comparison with healthy donors. The most significant changes were observed in the infertile men with leucocytospermia. This manifestation could be explained by the fact that white blood cells stimulate the formation of reactive oxygen species, induction and development of oxidative stress in sperm cells.http://publications.lnu.edu.ua/journals/index.php/biology/article/view/8antioxidant enzymeslipid peroxidationmale infertilitypathospermia |
| spellingShingle | R. V. Fafula О. K. Оnufrovych D. Z. Vorobets U. P. Iefremova Z. D. Vorobets Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia Біологічні студії antioxidant enzymes lipid peroxidation male infertility pathospermia |
| title | Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia |
| title_full | Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia |
| title_fullStr | Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia |
| title_full_unstemmed | Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia |
| title_short | Glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia |
| title_sort | glutathione antioxidant protection system in ejaculated spermatozoa of infertile men with different forms of pathospermia |
| topic | antioxidant enzymes lipid peroxidation male infertility pathospermia |
| url | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/8 |
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