The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression
abstract: The brown brocket deer is a species of neotropical deer that is found in several regions of Brazil. Although not considered an endangered species, it can be used as a model for studies with somatic cell nuclear transfer (SCNT) in endangered species. Thus, this study aimed to verify the emb...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Theriogenology Wild |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773093X22000010 |
| _version_ | 1797866931529711616 |
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| author | Luciana M. Melo Sâmara B. Silva Lívia C. Magalhães Jenin V. Cortez Satish Kumar José M.B. Duarte Luciana D. Rola Maiana S. Chaves Vicente J.F. Freitas |
| author_facet | Luciana M. Melo Sâmara B. Silva Lívia C. Magalhães Jenin V. Cortez Satish Kumar José M.B. Duarte Luciana D. Rola Maiana S. Chaves Vicente J.F. Freitas |
| author_sort | Luciana M. Melo |
| collection | DOAJ |
| description | abstract: The brown brocket deer is a species of neotropical deer that is found in several regions of Brazil. Although not considered an endangered species, it can be used as a model for studies with somatic cell nuclear transfer (SCNT) in endangered species. Thus, this study aimed to verify the embryo development and nuclear gene expression in intraSCNT (bovine-bovine), interSCNT (deer-bovine) and in vitro fertilized (IVF) bovine embryos. Thus, from a culture of brown brocket deer fibroblasts and enucleated bovine oocytes, inter and intraspecific embryos were produced. In addition, a group of IVF bovine embryos was prepared. Quantitative PCR (qPCR) was performed from the blastocysts obtained from each experimental group to analyze the expression of OCT4, T-FAM, GJA1, BAX and BCL2. The blastocyst rate was higher (P < 0.05) in IVF group (44.0%) when compared to intraSCNT (11.3%) and inter SCNT (5.9%). The expression of BCL2 was close to null and for GJA1 was similar (P > 0.05) among experimental groups. In conclusion, bovine cytoplast was able to reprogram the brown brocket deer karyoplast and these cloned embryos were able to reach the blastocyst stage. The inverse relationship for BAX and BCL2 can indicate a high degree of apoptosis in blastocysts from interSCNT. |
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| format | Article |
| id | doaj.art-b086601bd899409f9ef32765cde43113 |
| institution | Directory Open Access Journal |
| issn | 2773-093X |
| language | English |
| last_indexed | 2024-04-09T23:32:09Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Theriogenology Wild |
| spelling | doaj.art-b086601bd899409f9ef32765cde431132023-03-21T04:17:27ZengElsevierTheriogenology Wild2773-093X2022-01-011100001The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expressionLuciana M. Melo0Sâmara B. Silva1Lívia C. Magalhães2Jenin V. Cortez3Satish Kumar4José M.B. Duarte5Luciana D. Rola6Maiana S. Chaves7Vicente J.F. Freitas8Molecular Genetics Research Unit, University Center Fametro (UNIFAMETRO), 60010–260 Fortaleza, BrazilLaboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), 60714–903 Fortaleza, BrazilLaboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), 60714–903 Fortaleza, BrazilLaboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), 60714–903 Fortaleza, BrazilLaboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), 60714–903 Fortaleza, BrazilDepartment of Animal Science, Deer Research and Conservation Center, São Paulo State University (UNESP), 14884–900 Jaboticabal, BrazilDepartment of Animal Science, Paraíba Federal University (UFPB), 58397–000 Areia, BrazilLaboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), 60714–903 Fortaleza, BrazilLaboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), 60714–903 Fortaleza, Brazil; Correspondence to: Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University, Av. Dr Silas Munguba 1700, 60714–903 Fortaleza, Brazil.abstract: The brown brocket deer is a species of neotropical deer that is found in several regions of Brazil. Although not considered an endangered species, it can be used as a model for studies with somatic cell nuclear transfer (SCNT) in endangered species. Thus, this study aimed to verify the embryo development and nuclear gene expression in intraSCNT (bovine-bovine), interSCNT (deer-bovine) and in vitro fertilized (IVF) bovine embryos. Thus, from a culture of brown brocket deer fibroblasts and enucleated bovine oocytes, inter and intraspecific embryos were produced. In addition, a group of IVF bovine embryos was prepared. Quantitative PCR (qPCR) was performed from the blastocysts obtained from each experimental group to analyze the expression of OCT4, T-FAM, GJA1, BAX and BCL2. The blastocyst rate was higher (P < 0.05) in IVF group (44.0%) when compared to intraSCNT (11.3%) and inter SCNT (5.9%). The expression of BCL2 was close to null and for GJA1 was similar (P > 0.05) among experimental groups. In conclusion, bovine cytoplast was able to reprogram the brown brocket deer karyoplast and these cloned embryos were able to reach the blastocyst stage. The inverse relationship for BAX and BCL2 can indicate a high degree of apoptosis in blastocysts from interSCNT.http://www.sciencedirect.com/science/article/pii/S2773093X22000010BovineCervidCloningNuclear genes |
| spellingShingle | Luciana M. Melo Sâmara B. Silva Lívia C. Magalhães Jenin V. Cortez Satish Kumar José M.B. Duarte Luciana D. Rola Maiana S. Chaves Vicente J.F. Freitas The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression Theriogenology Wild Bovine Cervid Cloning Nuclear genes |
| title | The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression |
| title_full | The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression |
| title_fullStr | The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression |
| title_full_unstemmed | The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression |
| title_short | The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression |
| title_sort | use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast embryo development and nuclear gene expression |
| topic | Bovine Cervid Cloning Nuclear genes |
| url | http://www.sciencedirect.com/science/article/pii/S2773093X22000010 |
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