HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach
Ethnomedicinal plants have been employed for treatments in phytotherapy due to the quality, effectiveness, and assurance of herbal therapy. The present study aims to assess the different extracts (16 extracts) obtained from foliage, stem bark, root, and seeds by increased polarity solvent-based on e...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Results in Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715623000334 |
| _version_ | 1797799098987839488 |
|---|---|
| author | N.B. Krishnamurthy A.P. Ananda H.S. Nagendra Prasad P. Prabhuprasad N. Manju C.S. Karthik H.S. Jayanth T.V. Logaraj K.R. Savitha |
| author_facet | N.B. Krishnamurthy A.P. Ananda H.S. Nagendra Prasad P. Prabhuprasad N. Manju C.S. Karthik H.S. Jayanth T.V. Logaraj K.R. Savitha |
| author_sort | N.B. Krishnamurthy |
| collection | DOAJ |
| description | Ethnomedicinal plants have been employed for treatments in phytotherapy due to the quality, effectiveness, and assurance of herbal therapy. The present study aims to assess the different extracts (16 extracts) obtained from foliage, stem bark, root, and seeds by increased polarity solvent-based on extraction and analyze the antioxidant properties of Priva cordifolia (L.f) Druce plant. Total polyphenols and flavonoids have been found to range from 20.60 ± 0.19–148.20 ± 1.52 mg/g as gallic acid equivalent (GAE) and 7.31 ± 0.09–350.46 ± 12.80 mg/g as rutin equivalent (RE) respectively. All the extracts have been assessed for DPPH, ABTS scavenging radical and FRAP activity. Among these, methanol and acetone extract of the foliage showed excellent activity. The foliage extract was further fractioned and examined for antioxidant and antibacterial activity. A total of 27 metabolites were identified with the help of HRLC-MS. All the identified metabolites were tested for pharmacophore model, ADME, Blood-Brain Barrier, and Molecular docking analysis. In this insilico method, all compounds have given a good docking score and binding energy to the particular protein target, which ranges from − 6 to −10 against DNA gyrase protein (3G7B).The fraction was further evaluated for their cytotoxicity against normal cell L6 and revealed data showing insignificant toxicity. The foliage methanolic extract indicated a broad phytochemical in P. cordifolia and is yet to explore unknown vital constituents for the medicinal applications in the future. |
| first_indexed | 2024-03-13T04:13:52Z |
| format | Article |
| id | doaj.art-b088fca4e47b4c0288ba376d6b6de826 |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-13T04:13:52Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-b088fca4e47b4c0288ba376d6b6de8262023-06-21T06:52:03ZengElsevierResults in Chemistry2211-71562023-01-015100794HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approachN.B. Krishnamurthy0A.P. Ananda1H.S. Nagendra Prasad2P. Prabhuprasad3N. Manju4C.S. Karthik5H.S. Jayanth6T.V. Logaraj7K.R. Savitha8Department of Biotechnology, Shridevi Institute of Engineering & Technology, Tumkur 572106, Karnataka, IndiaCentre for Research and Evaluation, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; Ganesh Consultancy & Analytical Services, Mysuru 570016, Karnataka, India; Corresponding author at: Centre for Research and Evaluation, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.Department of Chemistry, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, Mysuru 570 006, Karnataka, IndiaCentre for Research and Evaluation, Bharathiar University, Coimbatore 641046, Tamil Nadu, IndiaDepartment of Chemistry, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, Mysuru 570 006, Karnataka, IndiaDepartment of Chemistry, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, Mysuru 570 006, Karnataka, IndiaDepartment of Microbiology Yuvaraja’s College, University of Mysuru, Mysuru 570005, Karnataka, IndiaPlant Science & Biotechnology, Government College of Arts & Science, Coimbatore 641018, Tamil Nadu, IndiaDepartment of Chemistry, University College of Science, Tumkur University, 572101, Karnataka, IndiaEthnomedicinal plants have been employed for treatments in phytotherapy due to the quality, effectiveness, and assurance of herbal therapy. The present study aims to assess the different extracts (16 extracts) obtained from foliage, stem bark, root, and seeds by increased polarity solvent-based on extraction and analyze the antioxidant properties of Priva cordifolia (L.f) Druce plant. Total polyphenols and flavonoids have been found to range from 20.60 ± 0.19–148.20 ± 1.52 mg/g as gallic acid equivalent (GAE) and 7.31 ± 0.09–350.46 ± 12.80 mg/g as rutin equivalent (RE) respectively. All the extracts have been assessed for DPPH, ABTS scavenging radical and FRAP activity. Among these, methanol and acetone extract of the foliage showed excellent activity. The foliage extract was further fractioned and examined for antioxidant and antibacterial activity. A total of 27 metabolites were identified with the help of HRLC-MS. All the identified metabolites were tested for pharmacophore model, ADME, Blood-Brain Barrier, and Molecular docking analysis. In this insilico method, all compounds have given a good docking score and binding energy to the particular protein target, which ranges from − 6 to −10 against DNA gyrase protein (3G7B).The fraction was further evaluated for their cytotoxicity against normal cell L6 and revealed data showing insignificant toxicity. The foliage methanolic extract indicated a broad phytochemical in P. cordifolia and is yet to explore unknown vital constituents for the medicinal applications in the future.http://www.sciencedirect.com/science/article/pii/S2211715623000334Priva cordifolia (L.f) Druce plantAntioxidant activityHR-LCMSBioactive compounds and Toxicology |
| spellingShingle | N.B. Krishnamurthy A.P. Ananda H.S. Nagendra Prasad P. Prabhuprasad N. Manju C.S. Karthik H.S. Jayanth T.V. Logaraj K.R. Savitha HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach Results in Chemistry Priva cordifolia (L.f) Druce plant Antioxidant activity HR-LCMS Bioactive compounds and Toxicology |
| title | HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach |
| title_full | HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach |
| title_fullStr | HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach |
| title_full_unstemmed | HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach |
| title_short | HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach |
| title_sort | hr lcms assisted phytochemical screening of antioxidant antibacterial activity of priva cordifolia l f druce plant and molecular docking approach |
| topic | Priva cordifolia (L.f) Druce plant Antioxidant activity HR-LCMS Bioactive compounds and Toxicology |
| url | http://www.sciencedirect.com/science/article/pii/S2211715623000334 |
| work_keys_str_mv | AT nbkrishnamurthy hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT apananda hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT hsnagendraprasad hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT pprabhuprasad hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT nmanju hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT cskarthik hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT hsjayanth hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT tvlogaraj hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach AT krsavitha hrlcmsassistedphytochemicalscreeningofantioxidantantibacterialactivityofprivacordifolialfdruceplantandmoleculardockingapproach |