NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial
Oxidative stress plays a major role in the pathogenesis of retinitis pigmentosa (RP). The main goal of this study was to evaluate the effect of 2-year nutritional intervention with antioxidant nutraceuticals on the visual function of RP patients. Secondly, we assessed how nutritional intervention af...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.847910/full |
| _version_ | 1819157909098987520 |
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| author | Lorena Olivares-González Lorena Olivares-González David Salom David Salom Emilio González-García David Hervás Natalia Mejía-Chiqui Mar Melero Sheyla Velasco Bianca Tabita Muresan Isabel Campillo Nieves Vila-Clérigues Eduardo López-Briz Juan Francisco Merino-Torres Juan Francisco Merino-Torres José María Millán José María Millán José María Millán José Miguel Soriano Del Castillo José Miguel Soriano Del Castillo Regina Rodrigo Regina Rodrigo Regina Rodrigo Regina Rodrigo Regina Rodrigo |
| author_facet | Lorena Olivares-González Lorena Olivares-González David Salom David Salom Emilio González-García David Hervás Natalia Mejía-Chiqui Mar Melero Sheyla Velasco Bianca Tabita Muresan Isabel Campillo Nieves Vila-Clérigues Eduardo López-Briz Juan Francisco Merino-Torres Juan Francisco Merino-Torres José María Millán José María Millán José María Millán José Miguel Soriano Del Castillo José Miguel Soriano Del Castillo Regina Rodrigo Regina Rodrigo Regina Rodrigo Regina Rodrigo Regina Rodrigo |
| author_sort | Lorena Olivares-González |
| collection | DOAJ |
| description | Oxidative stress plays a major role in the pathogenesis of retinitis pigmentosa (RP). The main goal of this study was to evaluate the effect of 2-year nutritional intervention with antioxidant nutraceuticals on the visual function of RP patients. Secondly, we assessed how nutritional intervention affected ocular and systemic redox status. We carried out a randomized, double-blind, placebo-controlled study. Thirty-one patients with RP participated in the study. RP patients randomly received either a mixture of nutraceuticals (NUT) containing folic acid, vitamin B6, vitamin A, zinc, copper, selenium, lutein, and zeaxanthin or placebo daily for 2 years. At baseline and after 2-year of the nutritional supplementation, visual function, dietetic-nutritional evaluations, serum concentration of nutraceuticals, plasma and aqueous humor concentration of several markers of redox status and inflammation were assessed. Retinal function and structure were assessed by multifocal electroretinogram (mfERG), spectral domain-optical coherence tomography (SD-OCT) and automated visual field (VF) tests. Nutritional status was estimated with validated questionnaires. Total antioxidant capacity, extracellular superoxide dismutase (SOD3), catalase (CAT), and glutathione peroxidase (GPx) activities, protein carbonyl adducts (CAR) content, thiobarbituric acid reactive substances (TBARS) formation (as indicator of lipid peroxidation), metabolites of the nitric oxide (NOX) and cytokine (interleukin 6 and tumor necrosis factor alpha) concentrations were assessed by biochemical and immunological techniques in aqueous humor or/and blood. Bayesian approach was performed to determine the probability of an effect. Region of practical equivalence (ROPE) was used. At baseline, Bayesian analysis revealed a high probability of an altered ocular redox status and to a lesser extent systemic redox status in RP patients compared to controls. Twenty-five patients (10 in the treated arm and 15 in the placebo arm) completed the nutritional intervention. After 2 years of supplementation, patients who received NUT presented better retinal responses (mfERG responses) compared to patients who received placebo. Besides, patients who received NUT showed better ocular antioxidant response (SOD3 activity) and lower oxidative damage (CAR) than those who received placebo. This study suggested that long-term NUT supplementation could slow down visual impairment and ameliorate ocular oxidative stress. |
| first_indexed | 2024-12-22T16:16:15Z |
| format | Article |
| id | doaj.art-b08be9e936184c4c8d61f01ee627b0b6 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-22T16:16:15Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-b08be9e936184c4c8d61f01ee627b0b62022-12-21T18:20:22ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-03-01910.3389/fnut.2022.847910847910NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled TrialLorena Olivares-González0Lorena Olivares-González1David Salom2David Salom3Emilio González-García4David Hervás5Natalia Mejía-Chiqui6Mar Melero7Sheyla Velasco8Bianca Tabita Muresan9Isabel Campillo10Nieves Vila-Clérigues11Eduardo López-Briz12Juan Francisco Merino-Torres13Juan Francisco Merino-Torres14José María Millán15José María Millán16José María Millán17José Miguel Soriano Del Castillo18José Miguel Soriano Del Castillo19Regina Rodrigo20Regina Rodrigo21Regina Rodrigo22Regina Rodrigo23Regina Rodrigo24Pathophysiology and Therapies for Vision Disorders, Principe Felipe Research Center (CIPF), Valencia, SpainJoint Research Unit on Rare Diseases CIPF-Health Research Institute Hospital La Fe (IIS-La Fe), Valencia, SpainDepartment of Ophthalmology, Manises Hospital, Manises, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), Madrid, SpainDepartment of Neuroscience, Manises Hospital, Manises, SpainDepartment of Applied Statistics, Operations Research and Quality, Universitat Politècnica de València, Valencia, SpainPathophysiology and Therapies for Vision Disorders, Principe Felipe Research Center (CIPF), Valencia, SpainService of Pharmacy, La Fe University and Polytechnic Hospital, Valencia, SpainPathophysiology and Therapies for Vision Disorders, Principe Felipe Research Center (CIPF), Valencia, SpainService of Endocrinology and Nutrition, University General Hospital, Valencia, SpainPathophysiology and Therapies for Vision Disorders, Principe Felipe Research Center (CIPF), Valencia, SpainService of Pharmacy, La Fe University and Polytechnic Hospital, Valencia, SpainService of Pharmacy, La Fe University and Polytechnic Hospital, Valencia, SpainService of Endocrinology and Nutrition, La Fe University and Polytechnic Hospital, Valencia, Spain0Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics UV-IIS La Fe, Valencia, SpainJoint Research Unit on Rare Diseases CIPF-Health Research Institute Hospital La Fe (IIS-La Fe), Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), Madrid, Spain1Molecular, Cellular and Genomic Biomedicine, IIS-La Fe, Valencia, Spain0Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics UV-IIS La Fe, Valencia, Spain2Food & Health Laboratory, Institute of Materials Science, University of Valencia (UV), Valencia, SpainPathophysiology and Therapies for Vision Disorders, Principe Felipe Research Center (CIPF), Valencia, SpainJoint Research Unit on Rare Diseases CIPF-Health Research Institute Hospital La Fe (IIS-La Fe), Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), Madrid, Spain0Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics UV-IIS La Fe, Valencia, Spain3Department of Physiology, University of Valencia (UV), Valencia, SpainOxidative stress plays a major role in the pathogenesis of retinitis pigmentosa (RP). The main goal of this study was to evaluate the effect of 2-year nutritional intervention with antioxidant nutraceuticals on the visual function of RP patients. Secondly, we assessed how nutritional intervention affected ocular and systemic redox status. We carried out a randomized, double-blind, placebo-controlled study. Thirty-one patients with RP participated in the study. RP patients randomly received either a mixture of nutraceuticals (NUT) containing folic acid, vitamin B6, vitamin A, zinc, copper, selenium, lutein, and zeaxanthin or placebo daily for 2 years. At baseline and after 2-year of the nutritional supplementation, visual function, dietetic-nutritional evaluations, serum concentration of nutraceuticals, plasma and aqueous humor concentration of several markers of redox status and inflammation were assessed. Retinal function and structure were assessed by multifocal electroretinogram (mfERG), spectral domain-optical coherence tomography (SD-OCT) and automated visual field (VF) tests. Nutritional status was estimated with validated questionnaires. Total antioxidant capacity, extracellular superoxide dismutase (SOD3), catalase (CAT), and glutathione peroxidase (GPx) activities, protein carbonyl adducts (CAR) content, thiobarbituric acid reactive substances (TBARS) formation (as indicator of lipid peroxidation), metabolites of the nitric oxide (NOX) and cytokine (interleukin 6 and tumor necrosis factor alpha) concentrations were assessed by biochemical and immunological techniques in aqueous humor or/and blood. Bayesian approach was performed to determine the probability of an effect. Region of practical equivalence (ROPE) was used. At baseline, Bayesian analysis revealed a high probability of an altered ocular redox status and to a lesser extent systemic redox status in RP patients compared to controls. Twenty-five patients (10 in the treated arm and 15 in the placebo arm) completed the nutritional intervention. After 2 years of supplementation, patients who received NUT presented better retinal responses (mfERG responses) compared to patients who received placebo. Besides, patients who received NUT showed better ocular antioxidant response (SOD3 activity) and lower oxidative damage (CAR) than those who received placebo. This study suggested that long-term NUT supplementation could slow down visual impairment and ameliorate ocular oxidative stress.https://www.frontiersin.org/articles/10.3389/fnut.2022.847910/fullretinitis pigmentosaoxidative damagenutraceuticalsantioxidantsvisual function |
| spellingShingle | Lorena Olivares-González Lorena Olivares-González David Salom David Salom Emilio González-García David Hervás Natalia Mejía-Chiqui Mar Melero Sheyla Velasco Bianca Tabita Muresan Isabel Campillo Nieves Vila-Clérigues Eduardo López-Briz Juan Francisco Merino-Torres Juan Francisco Merino-Torres José María Millán José María Millán José María Millán José Miguel Soriano Del Castillo José Miguel Soriano Del Castillo Regina Rodrigo Regina Rodrigo Regina Rodrigo Regina Rodrigo Regina Rodrigo NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial Frontiers in Nutrition retinitis pigmentosa oxidative damage nutraceuticals antioxidants visual function |
| title | NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial |
| title_full | NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial |
| title_fullStr | NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial |
| title_full_unstemmed | NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial |
| title_short | NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial |
| title_sort | nutraret effect of 2 year nutraceutical supplementation on redox status and visual function of patients with retinitis pigmentosa a randomized double blind placebo controlled trial |
| topic | retinitis pigmentosa oxidative damage nutraceuticals antioxidants visual function |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.847910/full |
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