Temporal and spatial differences in the vaginal microbiome of Chinese healthy women
Background Up the reproductive tract, there are large differences in the composition of vaginal microbes. Throughout the menstrual cycle, the structure of the vaginal microbiome shifts. Few studies have examined both in combination. Our study was designed to explore trends in the microbiome of diffe...
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PeerJ Inc.
2023-12-01
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| author | Limin Du Xue Dong Jiarong Song Tingting Lei Xianming Liu Yue Lan Xu Liu Jiao Wang Bisong Yue Miao He Zhenxin Fan Tao Guo |
| author_facet | Limin Du Xue Dong Jiarong Song Tingting Lei Xianming Liu Yue Lan Xu Liu Jiao Wang Bisong Yue Miao He Zhenxin Fan Tao Guo |
| author_sort | Limin Du |
| collection | DOAJ |
| description | Background Up the reproductive tract, there are large differences in the composition of vaginal microbes. Throughout the menstrual cycle, the structure of the vaginal microbiome shifts. Few studies have examined both in combination. Our study was designed to explore trends in the microbiome of different parts of the vagina in healthy women over the menstrual cycle. Methods We performed metagenomic sequencing to characterize the microbiome differences between the cervical orifice and mid-vagina throughout the menstrual cycle. Results Our results showed the vaginal microbiome of healthy women in the cervical orifice and the mid-vagina was similar during the periovulatory and luteal phases, with Lactobacillus being the dominant bacteria. In the follicular phase, Acinetobacter was detected in the cervical orifice. From the follicular phase to the luteal phase, the community state types (all five community status types were defined as CSTs) in samples No. 10 and No. 11 changed from CST III to CST I. In addition, the composition of the vaginal microbiome in healthy women from different regions of China was significantly different. We also detected viruses including Human alphaherpesvirus 1 (HSV-1) during periovulatory phase. Conclusion This study is valuable for understanding whether the microbial composition of the vagina is consistent in different parts of the menstrual cycle. |
| first_indexed | 2024-03-09T03:23:46Z |
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| id | doaj.art-b08e0c150c544cf493f3cf7d9d0db120 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T03:23:46Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-b08e0c150c544cf493f3cf7d9d0db1202023-12-03T15:05:08ZengPeerJ Inc.PeerJ2167-83592023-12-0111e1643810.7717/peerj.16438Temporal and spatial differences in the vaginal microbiome of Chinese healthy womenLimin Du0Xue Dong1Jiarong Song2Tingting Lei3Xianming Liu4Yue Lan5Xu Liu6Jiao Wang7Bisong Yue8Miao He9Zhenxin Fan10Tao Guo11Sichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaAmbulatory Surgery Department, West China Second Hospital, Sichuan University, Chengdu, ChinaSichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaSuining Municipal Hospital of Traditional Chinese Medicine, Suining, Sichuan, ChinaMianyang Tumor Hospital, Sichuan Province, Mianyang, ChinaSichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaSichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaSichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaSichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, ChinaSichuan University, Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Chengdu, ChinaDepartment of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, ChinaBackground Up the reproductive tract, there are large differences in the composition of vaginal microbes. Throughout the menstrual cycle, the structure of the vaginal microbiome shifts. Few studies have examined both in combination. Our study was designed to explore trends in the microbiome of different parts of the vagina in healthy women over the menstrual cycle. Methods We performed metagenomic sequencing to characterize the microbiome differences between the cervical orifice and mid-vagina throughout the menstrual cycle. Results Our results showed the vaginal microbiome of healthy women in the cervical orifice and the mid-vagina was similar during the periovulatory and luteal phases, with Lactobacillus being the dominant bacteria. In the follicular phase, Acinetobacter was detected in the cervical orifice. From the follicular phase to the luteal phase, the community state types (all five community status types were defined as CSTs) in samples No. 10 and No. 11 changed from CST III to CST I. In addition, the composition of the vaginal microbiome in healthy women from different regions of China was significantly different. We also detected viruses including Human alphaherpesvirus 1 (HSV-1) during periovulatory phase. Conclusion This study is valuable for understanding whether the microbial composition of the vagina is consistent in different parts of the menstrual cycle.https://peerj.com/articles/16438.pdfVagina microbiomeMenstrual cycleMetagenomic sequencingLactobacillusHealthy women |
| spellingShingle | Limin Du Xue Dong Jiarong Song Tingting Lei Xianming Liu Yue Lan Xu Liu Jiao Wang Bisong Yue Miao He Zhenxin Fan Tao Guo Temporal and spatial differences in the vaginal microbiome of Chinese healthy women PeerJ Vagina microbiome Menstrual cycle Metagenomic sequencing Lactobacillus Healthy women |
| title | Temporal and spatial differences in the vaginal microbiome of Chinese healthy women |
| title_full | Temporal and spatial differences in the vaginal microbiome of Chinese healthy women |
| title_fullStr | Temporal and spatial differences in the vaginal microbiome of Chinese healthy women |
| title_full_unstemmed | Temporal and spatial differences in the vaginal microbiome of Chinese healthy women |
| title_short | Temporal and spatial differences in the vaginal microbiome of Chinese healthy women |
| title_sort | temporal and spatial differences in the vaginal microbiome of chinese healthy women |
| topic | Vagina microbiome Menstrual cycle Metagenomic sequencing Lactobacillus Healthy women |
| url | https://peerj.com/articles/16438.pdf |
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