New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae)
Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for <i>Prosopis</i> species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obta...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-05-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1200324 |
| _version_ | 1818138909479010304 |
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| author | Cecilia F. Bessega Carolina L. Pometti Joe T. Miller Richard Watts Beatriz O. Saidman Juan C. Vilardi |
| author_facet | Cecilia F. Bessega Carolina L. Pometti Joe T. Miller Richard Watts Beatriz O. Saidman Juan C. Vilardi |
| author_sort | Cecilia F. Bessega |
| collection | DOAJ |
| description | Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for <i>Prosopis</i> species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for <i>Prosopis</i> species from a 454 pyrosequencing run were optimized and characterized for studies in <i>P. alba</i> and <i>P. chilensis</i>.
Methods and Results: Twelve markers that were successfully amplified showed polymorphism in <i>P. alba</i> and <i>P. chilensis</i>. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in <i>P. ruscifolia</i>, <i>P. flexuosa</i>, <i>P. kuntzei</i>, <i>P. glandulosa</i>, and <i>P. pallida</i>.
Conclusions: These loci will enable genetic diversity studies of <i>P. alba</i> and <i>P. chilensis</i> and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection. |
| first_indexed | 2024-12-11T10:19:41Z |
| format | Article |
| id | doaj.art-b08e47ef99b844f993ccde91405681ad |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-11T10:19:41Z |
| publishDate | 2013-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-b08e47ef99b844f993ccde91405681ad2022-12-22T01:11:29ZengWileyApplications in Plant Sciences2168-04502013-05-0115120032410.3732/apps.1200324New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae)Cecilia F. Bessega0Carolina L. Pometti1Joe T. Miller2Richard Watts3Beatriz O. Saidman4Juan C. Vilardi5Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, ArgentinaLaboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, ArgentinaCentre for Australian National Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2601, AustraliaCentre for Australian National Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2601, AustraliaLaboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, ArgentinaLaboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, ArgentinaPremise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for <i>Prosopis</i> species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for <i>Prosopis</i> species from a 454 pyrosequencing run were optimized and characterized for studies in <i>P. alba</i> and <i>P. chilensis</i>. Methods and Results: Twelve markers that were successfully amplified showed polymorphism in <i>P. alba</i> and <i>P. chilensis</i>. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in <i>P. ruscifolia</i>, <i>P. flexuosa</i>, <i>P. kuntzei</i>, <i>P. glandulosa</i>, and <i>P. pallida</i>. Conclusions: These loci will enable genetic diversity studies of <i>P. alba</i> and <i>P. chilensis</i> and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.http://www.bioone.org/doi/full/10.3732/apps.1200324Fabaceaemicrosatellitenext-generation sequencing dataProsopis |
| spellingShingle | Cecilia F. Bessega Carolina L. Pometti Joe T. Miller Richard Watts Beatriz O. Saidman Juan C. Vilardi New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae) Applications in Plant Sciences Fabaceae microsatellite next-generation sequencing data Prosopis |
| title | New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae) |
| title_full | New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae) |
| title_fullStr | New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae) |
| title_full_unstemmed | New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae) |
| title_short | New Microsatellite Loci for <i>Prosopis alba</i> and <i>P. chilensis</i> (Fabaceae) |
| title_sort | new microsatellite loci for i prosopis alba i and i p chilensis i fabaceae |
| topic | Fabaceae microsatellite next-generation sequencing data Prosopis |
| url | http://www.bioone.org/doi/full/10.3732/apps.1200324 |
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