Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells.
Hydrogen sulfide (H(2)S) can be endogenously generated from cystathionine gamma-lyase (CSE) in cardiovascular system, offering a cardiovascular protection. It is also known that the lower risk of cardiovascular diseases in female is partially attributed to the protective effect of estrogen. The curr...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870237/?tool=EBI |
| _version_ | 1818406081076920320 |
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| author | Hongzhu Li Sarathi Mani Wei Cao Guangdong Yang Christopher Lai Lingyun Wu Rui Wang |
| author_facet | Hongzhu Li Sarathi Mani Wei Cao Guangdong Yang Christopher Lai Lingyun Wu Rui Wang |
| author_sort | Hongzhu Li |
| collection | DOAJ |
| description | Hydrogen sulfide (H(2)S) can be endogenously generated from cystathionine gamma-lyase (CSE) in cardiovascular system, offering a cardiovascular protection. It is also known that the lower risk of cardiovascular diseases in female is partially attributed to the protective effect of estrogen. The current study explores the interaction of H(2)S and estrogen on smooth muscle cell (SMC) growth. In the present study, we found that the proliferation of cultured vascular SMCs isolated from wild-type mice (WT-SMCs) was inhibited, but that from CSE gene knockout mice (CSE-KO-SMCs) increased, by estrogen treatments. The expression of estrogen receptor α (ERα), but not ERβ, was significantly decreased in CSE-KO-SMCs compared with that in WT-SMCs. Exogenously applied H(2)S markedly increased ERα but not ERβ expression. In addition, the inhibition of ER activation and knockdown of ERα expression in WT-SMCs or the overexpression of ERα in CSE-KO-SMCs reversed the respective effects of estrogen on cell proliferation. The expression of cyclin D1 was reduced in WT-SMCs but increased in CSE-KO-SMCs after estrogen treatments, which was reversed by knockdown of ERα in WT-SMCs or overexpression of ERα in CSE-KO-SMCs, respectively. The overexpression of cyclin D1 in WT-SMCs or knockdown of cyclin D1 expression in CSE-KO-SMCs reversed the effects of estrogen on cell proliferation. These results suggest that H(2)S mediates estrogen-inhibited proliferation of SMCs via selective activation of ERα/cyclin D1 pathways. |
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| format | Article |
| id | doaj.art-b08f34aa797c4087be1edf7c5fc732d0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T09:06:16Z |
| publishDate | 2012-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-b08f34aa797c4087be1edf7c5fc732d02022-12-21T23:08:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4161410.1371/journal.pone.0041614Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells.Hongzhu LiSarathi ManiWei CaoGuangdong YangChristopher LaiLingyun WuRui WangHydrogen sulfide (H(2)S) can be endogenously generated from cystathionine gamma-lyase (CSE) in cardiovascular system, offering a cardiovascular protection. It is also known that the lower risk of cardiovascular diseases in female is partially attributed to the protective effect of estrogen. The current study explores the interaction of H(2)S and estrogen on smooth muscle cell (SMC) growth. In the present study, we found that the proliferation of cultured vascular SMCs isolated from wild-type mice (WT-SMCs) was inhibited, but that from CSE gene knockout mice (CSE-KO-SMCs) increased, by estrogen treatments. The expression of estrogen receptor α (ERα), but not ERβ, was significantly decreased in CSE-KO-SMCs compared with that in WT-SMCs. Exogenously applied H(2)S markedly increased ERα but not ERβ expression. In addition, the inhibition of ER activation and knockdown of ERα expression in WT-SMCs or the overexpression of ERα in CSE-KO-SMCs reversed the respective effects of estrogen on cell proliferation. The expression of cyclin D1 was reduced in WT-SMCs but increased in CSE-KO-SMCs after estrogen treatments, which was reversed by knockdown of ERα in WT-SMCs or overexpression of ERα in CSE-KO-SMCs, respectively. The overexpression of cyclin D1 in WT-SMCs or knockdown of cyclin D1 expression in CSE-KO-SMCs reversed the effects of estrogen on cell proliferation. These results suggest that H(2)S mediates estrogen-inhibited proliferation of SMCs via selective activation of ERα/cyclin D1 pathways.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870237/?tool=EBI |
| spellingShingle | Hongzhu Li Sarathi Mani Wei Cao Guangdong Yang Christopher Lai Lingyun Wu Rui Wang Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells. PLoS ONE |
| title | Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells. |
| title_full | Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells. |
| title_fullStr | Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells. |
| title_full_unstemmed | Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells. |
| title_short | Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells. |
| title_sort | interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870237/?tool=EBI |
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