Immune stimulation reduces sleep and memory ability in Drosophila melanogaster
Psychoneuroimmunology studies the increasing number of connections between neurobiology, immunology and behaviour. We demonstrate the effects of the immune response on two fundamental behaviours: sleep and memory ability in Drosophila melanogaster. We used the Geneswitch system to upregulate peptido...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2014-06-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/434.pdf |
| _version_ | 1797424639464439808 |
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| author | Eamonn B. Mallon Akram Alghamdi Robert T.K. Holdbrook Ezio Rosato |
| author_facet | Eamonn B. Mallon Akram Alghamdi Robert T.K. Holdbrook Ezio Rosato |
| author_sort | Eamonn B. Mallon |
| collection | DOAJ |
| description | Psychoneuroimmunology studies the increasing number of connections between neurobiology, immunology and behaviour. We demonstrate the effects of the immune response on two fundamental behaviours: sleep and memory ability in Drosophila melanogaster. We used the Geneswitch system to upregulate peptidoglycan receptor protein (PGRP) expression, thereby stimulating the immune system in the absence of infection. Geneswitch was activated by feeding the steroid RU486, to the flies. We used an aversive classical conditioning paradigm to quantify memory and measures of activity to infer sleep. Immune stimulated flies exhibited reduced levels of sleep, which could not be explained by a generalised increase in waking activity. Immune stimulated flies also showed a reduction in memory abilities. These results lend support to Drosophila as a model for immune–neural interactions and provide a possible role for sleep in the interplay between the immune response and memory. |
| first_indexed | 2024-03-09T08:04:43Z |
| format | Article |
| id | doaj.art-b090ad504fa54c239cdda579243551df |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:04:43Z |
| publishDate | 2014-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-b090ad504fa54c239cdda579243551df2023-12-03T00:25:10ZengPeerJ Inc.PeerJ2167-83592014-06-012e43410.7717/peerj.434434Immune stimulation reduces sleep and memory ability in Drosophila melanogasterEamonn B. Mallon0Akram Alghamdi1Robert T.K. Holdbrook2Ezio Rosato3Department of Biology, University of Leicester, Leicester, United KingdomDepartment of Biology, Taif University, Saudi ArabiaDepartment of Biology, University of Leicester, Leicester, United KingdomDepartment of Genetics, University of Leicester, Leicester, United KingdomPsychoneuroimmunology studies the increasing number of connections between neurobiology, immunology and behaviour. We demonstrate the effects of the immune response on two fundamental behaviours: sleep and memory ability in Drosophila melanogaster. We used the Geneswitch system to upregulate peptidoglycan receptor protein (PGRP) expression, thereby stimulating the immune system in the absence of infection. Geneswitch was activated by feeding the steroid RU486, to the flies. We used an aversive classical conditioning paradigm to quantify memory and measures of activity to infer sleep. Immune stimulated flies exhibited reduced levels of sleep, which could not be explained by a generalised increase in waking activity. Immune stimulated flies also showed a reduction in memory abilities. These results lend support to Drosophila as a model for immune–neural interactions and provide a possible role for sleep in the interplay between the immune response and memory.https://peerj.com/articles/434.pdfPsychoneuroimmunologyFruit flyImmune–neural interactionsImdPGRP-LCaGeneswitch |
| spellingShingle | Eamonn B. Mallon Akram Alghamdi Robert T.K. Holdbrook Ezio Rosato Immune stimulation reduces sleep and memory ability in Drosophila melanogaster PeerJ Psychoneuroimmunology Fruit fly Immune–neural interactions Imd PGRP-LCa Geneswitch |
| title | Immune stimulation reduces sleep and memory ability in Drosophila melanogaster |
| title_full | Immune stimulation reduces sleep and memory ability in Drosophila melanogaster |
| title_fullStr | Immune stimulation reduces sleep and memory ability in Drosophila melanogaster |
| title_full_unstemmed | Immune stimulation reduces sleep and memory ability in Drosophila melanogaster |
| title_short | Immune stimulation reduces sleep and memory ability in Drosophila melanogaster |
| title_sort | immune stimulation reduces sleep and memory ability in drosophila melanogaster |
| topic | Psychoneuroimmunology Fruit fly Immune–neural interactions Imd PGRP-LCa Geneswitch |
| url | https://peerj.com/articles/434.pdf |
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