Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure
Comparative developing brain histo-anatomical pathologies of Fluoride ions and Bifenthrin in-ovo exposures were explored in the golden black variety of domestic chick. Three exposure groups were –the Vehicle control group (Vg); Fluoride (F) group and the Bifenthrin (Bn) group each with forty fertili...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750023000124 |
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| author | Ayesha Faisal Zubedah Khanum Syeda Nadia Ahmad Khawaja Raees Ahmad Asma Younis Sadia Suleman Iram Inayat Muhammad Ali Kanwal |
| author_facet | Ayesha Faisal Zubedah Khanum Syeda Nadia Ahmad Khawaja Raees Ahmad Asma Younis Sadia Suleman Iram Inayat Muhammad Ali Kanwal |
| author_sort | Ayesha Faisal |
| collection | DOAJ |
| description | Comparative developing brain histo-anatomical pathologies of Fluoride ions and Bifenthrin in-ovo exposures were explored in the golden black variety of domestic chick. Three exposure groups were –the Vehicle control group (Vg); Fluoride (F) group and the Bifenthrin (Bn) group each with forty fertilized eggs and received their respective group treatment at zero day of incubation. Embryos were extracted, dissected from head region and the embryonic whole brains were recovered after 14 days of incubation. The embryonic brains were preserved in bouin fixative for 24 h for further studies. The morphological results show the atrophied and hypertrophied embryonic brain in F and Bn groups respectively as compared to Vg group. The toxicological signs of encephalic anatomy and histology of F and Bn exposure were the enlarged third ventricles, optocoeles and arachnoid mater, encephalic spongiosis and decreased neuroglial density. The morphometric data showed significant decrease (p ≤ 0.05) in mean weight and density of whole brain in F and Bn groups compared Vg. The mean length and width of whole brain in F were significantly lower than that of the Bn and Vg. whereas, the mean breadth of third ventricle in Bn remained significantly lower than F and Vg groups. On the other hand, the mean breadth of optocoele and fourth ventricle in F and Bn groups remained significantly higher than Vg. Conversely the mean optic lobe wall thickness in F remained significantly lower than Bn and Vg. Additionally, the mean neuronal density in diencephalon, optic lobe and cerebellum in F group and Bn group remained significantly (p ≤ 0.05) lower than Vg. Results show that low dose in-ovo fluoride or bifenthrin exposure may cause neuro-developmental abnormalities in the developing chick embryos indicating that the Fluoride-ions and Bifenthrin harbor strong developmental neurotoxic capacity. |
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| format | Article |
| id | doaj.art-b093d86e35d34304ad0b57554a18fa3a |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-03-13T04:11:01Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
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| series | Toxicology Reports |
| spelling | doaj.art-b093d86e35d34304ad0b57554a18fa3a2023-06-21T06:55:03ZengElsevierToxicology Reports2214-75002023-01-0110190194Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposureAyesha Faisal0Zubedah Khanum1Syeda Nadia Ahmad2Khawaja Raees Ahmad3Asma Younis4Sadia Suleman5Iram Inayat6Muhammad Ali Kanwal7Department of Zoology, University of Sargodha, Sargodha 40100, PakistanDepartment of Zoology, University of Sargodha, Sargodha 40100, PakistanDepartment of Zoology, University of Chakwal, Chakwal 48800, Pakistan; Corresponding author.Department of Zoology, University of Sargodha, Sargodha 40100, PakistanDepartment of Zoology, University of Sargodha, Sargodha 40100, PakistanDepartment of Zoology, University of Sargodha, Sargodha 40100, PakistanDepartment of Zoology, University of Sargodha, Sargodha 40100, PakistanDepartment of Zoology, University of Sargodha, Sargodha 40100, PakistanComparative developing brain histo-anatomical pathologies of Fluoride ions and Bifenthrin in-ovo exposures were explored in the golden black variety of domestic chick. Three exposure groups were –the Vehicle control group (Vg); Fluoride (F) group and the Bifenthrin (Bn) group each with forty fertilized eggs and received their respective group treatment at zero day of incubation. Embryos were extracted, dissected from head region and the embryonic whole brains were recovered after 14 days of incubation. The embryonic brains were preserved in bouin fixative for 24 h for further studies. The morphological results show the atrophied and hypertrophied embryonic brain in F and Bn groups respectively as compared to Vg group. The toxicological signs of encephalic anatomy and histology of F and Bn exposure were the enlarged third ventricles, optocoeles and arachnoid mater, encephalic spongiosis and decreased neuroglial density. The morphometric data showed significant decrease (p ≤ 0.05) in mean weight and density of whole brain in F and Bn groups compared Vg. The mean length and width of whole brain in F were significantly lower than that of the Bn and Vg. whereas, the mean breadth of third ventricle in Bn remained significantly lower than F and Vg groups. On the other hand, the mean breadth of optocoele and fourth ventricle in F and Bn groups remained significantly higher than Vg. Conversely the mean optic lobe wall thickness in F remained significantly lower than Bn and Vg. Additionally, the mean neuronal density in diencephalon, optic lobe and cerebellum in F group and Bn group remained significantly (p ≤ 0.05) lower than Vg. Results show that low dose in-ovo fluoride or bifenthrin exposure may cause neuro-developmental abnormalities in the developing chick embryos indicating that the Fluoride-ions and Bifenthrin harbor strong developmental neurotoxic capacity.http://www.sciencedirect.com/science/article/pii/S2214750023000124FluorideBifenthrinDevelopmental NeurotoxicityChick |
| spellingShingle | Ayesha Faisal Zubedah Khanum Syeda Nadia Ahmad Khawaja Raees Ahmad Asma Younis Sadia Suleman Iram Inayat Muhammad Ali Kanwal Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure Toxicology Reports Fluoride Bifenthrin Developmental Neurotoxicity Chick |
| title | Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure |
| title_full | Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure |
| title_fullStr | Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure |
| title_full_unstemmed | Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure |
| title_short | Histo-anatomical mutilations of developing chick brain induced by in-ovo fluoride and bifenthrin exposure |
| title_sort | histo anatomical mutilations of developing chick brain induced by in ovo fluoride and bifenthrin exposure |
| topic | Fluoride Bifenthrin Developmental Neurotoxicity Chick |
| url | http://www.sciencedirect.com/science/article/pii/S2214750023000124 |
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