Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model
Background: Microdissection testicular sperm extraction (micro-TESE) has replaced conventional testis biopsies as a method of choice for obtaining sperm for in vitro fertilization for men with nonobstructive azoospermia. A technical challenge of micro-TESE is that the low magnification inspection of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2012;volume=3;issue=1;spage=4;epage=4;aulast=Ramasamy |
| _version_ | 1818265652502200320 |
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| author | Ranjith Ramasamy Joshua Sterling Maryem Manzoor Bekheit Salamoon Manu Jain Erik Fisher Phillip S Li Peter N Schlegel Sushmita Mukherjee |
| author_facet | Ranjith Ramasamy Joshua Sterling Maryem Manzoor Bekheit Salamoon Manu Jain Erik Fisher Phillip S Li Peter N Schlegel Sushmita Mukherjee |
| author_sort | Ranjith Ramasamy |
| collection | DOAJ |
| description | Background: Microdissection testicular sperm extraction (micro-TESE) has replaced conventional testis biopsies as a method of choice for obtaining sperm for in vitro fertilization for men with nonobstructive azoospermia. A technical challenge of micro-TESE is that the low magnification inspection of the tubules with a surgical microscope is insufficient to definitively identify sperm-containing tubules, necessitating tissue removal and cytologic assessment. Full field optical coherence tomography (FFOCT) uses white light interference microscopy to generate quick high-resolution tomographic images of fresh (unprocessed and unstained) tissue. Furthermore, by using a nonlaser safe light source (150 W halogen lamp) for tissue illumination, it ensures that the sperm extracted for in vitro fertilization are not photo-damaged or mutagenized. Materials and Methods: A focal Sertoli-cell only rodent model was created with busulfan injection in adult rats. Ex vivo testicular tissues from both normal and busulfan-treated rats were imaged with a commercial modified FFOCT system, Light-CT TM , and the images were correlated with gold standard hematoxylin and eosin staining. Results: Light-CT TM identified spermatogenesis within the seminiferous tubules in freshly excised testicular tissue, without the use of exogenous contrast or fixation. Normal adult rats exhibited tubules with uniform size and shape (diameter 328 ±11 μm). The busulfan-treated animals showed marked heterogeneity in tubular size and shape (diameter 178 ± 35 μm) and only 10% contained sperm within the lumen. Conclusion : FFOCT has the potential to facilitate real-time visualization of spermatogenesis in humans, and aid in micro-TESE for men with infertility. |
| first_indexed | 2024-12-12T19:54:13Z |
| format | Article |
| id | doaj.art-b099df1b5a4d4ba9840c77459562653f |
| institution | Directory Open Access Journal |
| issn | 2153-3539 2153-3539 |
| language | English |
| last_indexed | 2024-12-12T19:54:13Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-b099df1b5a4d4ba9840c77459562653f2022-12-22T00:13:56ZengElsevierJournal of Pathology Informatics2153-35392153-35392012-01-01314410.4103/2153-3539.93401Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only modelRanjith RamasamyJoshua SterlingMaryem ManzoorBekheit SalamoonManu JainErik FisherPhillip S LiPeter N SchlegelSushmita MukherjeeBackground: Microdissection testicular sperm extraction (micro-TESE) has replaced conventional testis biopsies as a method of choice for obtaining sperm for in vitro fertilization for men with nonobstructive azoospermia. A technical challenge of micro-TESE is that the low magnification inspection of the tubules with a surgical microscope is insufficient to definitively identify sperm-containing tubules, necessitating tissue removal and cytologic assessment. Full field optical coherence tomography (FFOCT) uses white light interference microscopy to generate quick high-resolution tomographic images of fresh (unprocessed and unstained) tissue. Furthermore, by using a nonlaser safe light source (150 W halogen lamp) for tissue illumination, it ensures that the sperm extracted for in vitro fertilization are not photo-damaged or mutagenized. Materials and Methods: A focal Sertoli-cell only rodent model was created with busulfan injection in adult rats. Ex vivo testicular tissues from both normal and busulfan-treated rats were imaged with a commercial modified FFOCT system, Light-CT TM , and the images were correlated with gold standard hematoxylin and eosin staining. Results: Light-CT TM identified spermatogenesis within the seminiferous tubules in freshly excised testicular tissue, without the use of exogenous contrast or fixation. Normal adult rats exhibited tubules with uniform size and shape (diameter 328 ±11 μm). The busulfan-treated animals showed marked heterogeneity in tubular size and shape (diameter 178 ± 35 μm) and only 10% contained sperm within the lumen. Conclusion : FFOCT has the potential to facilitate real-time visualization of spermatogenesis in humans, and aid in micro-TESE for men with infertility.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2012;volume=3;issue=1;spage=4;epage=4;aulast=Ramasamymicro-TESErat modelsertoli cell onlytestis |
| spellingShingle | Ranjith Ramasamy Joshua Sterling Maryem Manzoor Bekheit Salamoon Manu Jain Erik Fisher Phillip S Li Peter N Schlegel Sushmita Mukherjee Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model Journal of Pathology Informatics micro-TESE rat model sertoli cell only testis |
| title | Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model |
| title_full | Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model |
| title_fullStr | Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model |
| title_full_unstemmed | Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model |
| title_short | Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model |
| title_sort | full field optical coherence tomography can identify spermatogenesis in a rodent sertoli cell only model |
| topic | micro-TESE rat model sertoli cell only testis |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2012;volume=3;issue=1;spage=4;epage=4;aulast=Ramasamy |
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