Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy
Abstract The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy. The ability to precisely target and edit specific genetic mutations that drive the growth and spread of tumors has opened up new possibilities for the d...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12943-023-01925-5 |
| _version_ | 1827328393376432128 |
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| author | Mohammad Chehelgerdi Matin Chehelgerdi Milad Khorramian-Ghahfarokhi Marjan Shafieizadeh Esmaeil Mahmoudi Fatemeh Eskandari Mohsen Rashidi Asghar Arshi Abbas Mokhtari-Farsani |
| author_facet | Mohammad Chehelgerdi Matin Chehelgerdi Milad Khorramian-Ghahfarokhi Marjan Shafieizadeh Esmaeil Mahmoudi Fatemeh Eskandari Mohsen Rashidi Asghar Arshi Abbas Mokhtari-Farsani |
| author_sort | Mohammad Chehelgerdi |
| collection | DOAJ |
| description | Abstract The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy. The ability to precisely target and edit specific genetic mutations that drive the growth and spread of tumors has opened up new possibilities for the development of more effective and personalized cancer treatments. In this review, we will discuss the different CRISPR-based strategies that have been proposed for cancer therapy, including inactivating genes that drive tumor growth, enhancing the immune response to cancer cells, repairing genetic mutations that cause cancer, and delivering cancer-killing molecules directly to tumor cells. We will also summarize the current state of preclinical studies and clinical trials of CRISPR-based cancer therapy, highlighting the most promising results and the challenges that still need to be overcome. Safety and delivery are also important challenges for CRISPR-based cancer therapy to become a viable clinical option. We will discuss the challenges and limitations that need to be overcome, such as off-target effects, safety, and delivery to the tumor site. Finally, we will provide an overview of the current challenges and opportunities in the field of CRISPR-based cancer therapy and discuss future directions for research and development. The CRISPR system has the potential to change the landscape of cancer research, and this review aims to provide an overview of the current state of the field and the challenges that need to be overcome to realize this potential. |
| first_indexed | 2024-03-07T15:17:18Z |
| format | Article |
| id | doaj.art-b0a07b646ad7494e99b114c432917022 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-03-07T15:17:18Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-b0a07b646ad7494e99b114c4329170222024-03-05T17:53:00ZengBMCMolecular Cancer1476-45982024-01-0123114510.1186/s12943-023-01925-5Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapyMohammad Chehelgerdi0Matin Chehelgerdi1Milad Khorramian-Ghahfarokhi2Marjan Shafieizadeh3Esmaeil Mahmoudi4Fatemeh Eskandari5Mohsen Rashidi6Asghar Arshi7Abbas Mokhtari-Farsani8Novin Genome (NG) Lab, Research and Development Center for BiotechnologyNovin Genome (NG) Lab, Research and Development Center for BiotechnologyDivision of Biotechnology, Department of Pathobiology, School of Veterinary Medicine, Shiraz UniversityDepartment of Chemistry, Shahid Bahonar University of KermanYoung Researchers and Elite Club, Shahrekord Branch, Islamic Azad UniversityFaculty of Molecular and Cellular Biology -Genetics, Islamic Azad University of FalavarjanDepartment Pharmacology, Faculty of Medicine, Mazandaran University of Medical SciencesYoung Researchers and Elite Club, Najafabad Branch, Islamic Azad UniversityNovin Genome (NG) Lab, Research and Development Center for BiotechnologyAbstract The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy. The ability to precisely target and edit specific genetic mutations that drive the growth and spread of tumors has opened up new possibilities for the development of more effective and personalized cancer treatments. In this review, we will discuss the different CRISPR-based strategies that have been proposed for cancer therapy, including inactivating genes that drive tumor growth, enhancing the immune response to cancer cells, repairing genetic mutations that cause cancer, and delivering cancer-killing molecules directly to tumor cells. We will also summarize the current state of preclinical studies and clinical trials of CRISPR-based cancer therapy, highlighting the most promising results and the challenges that still need to be overcome. Safety and delivery are also important challenges for CRISPR-based cancer therapy to become a viable clinical option. We will discuss the challenges and limitations that need to be overcome, such as off-target effects, safety, and delivery to the tumor site. Finally, we will provide an overview of the current challenges and opportunities in the field of CRISPR-based cancer therapy and discuss future directions for research and development. The CRISPR system has the potential to change the landscape of cancer research, and this review aims to provide an overview of the current state of the field and the challenges that need to be overcome to realize this potential.https://doi.org/10.1186/s12943-023-01925-5CRISPR systemGenome editingCancer therapyGenetic mutationsTumor growthImmune response |
| spellingShingle | Mohammad Chehelgerdi Matin Chehelgerdi Milad Khorramian-Ghahfarokhi Marjan Shafieizadeh Esmaeil Mahmoudi Fatemeh Eskandari Mohsen Rashidi Asghar Arshi Abbas Mokhtari-Farsani Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy Molecular Cancer CRISPR system Genome editing Cancer therapy Genetic mutations Tumor growth Immune response |
| title | Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy |
| title_full | Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy |
| title_fullStr | Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy |
| title_full_unstemmed | Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy |
| title_short | Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy |
| title_sort | comprehensive review of crispr based gene editing mechanisms challenges and applications in cancer therapy |
| topic | CRISPR system Genome editing Cancer therapy Genetic mutations Tumor growth Immune response |
| url | https://doi.org/10.1186/s12943-023-01925-5 |
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